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  • 1
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A genetic map of Lolium has been produced using isozyme, restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers applied to a segregating family derived from an F1 hybrid plant of L. perenne × L. multiflorum provenance, crossed on to a doubled haploid L. perenne. A total of 106 markers, out of a total of 160 polymorphic loci analysed, have been ascribed to seven linkage groups covering a map distance of 692cM, Two of these groups may be allocated to chromosomes 2 and 6 of the Lolium genome.The remaining unallocated markers, the majority of which showed severe segregation distortion, could be associated into small groups of two or three markers which showed no linkage with the main groups at a LOD of 2.8 or, if associated, could not be mapped in a satisfactory manner. This high incidence of disturbed segregations could be accounted for by the use of an interspecific hybrid between two species of differing genome size, with consequent cytological imbalance.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 104 (1990), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The segregation of isozyme markers has been examined in the green and albino androgentic progenies of two plants of Lolium perenne. Overall the pooled data for the loci PG1/2 and GOT/2 does not deviate from expectation, but there is considerable heterogeneity between families and between green and albino plants. Specific alleles predominate in the different progeny sets. The results may be accounted for by possible linked loci affecting anther culture response or of linked lethal genes.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 103 (1989), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A Triple Test Cross and basic generations (i.e. inbred parents, F1′s, F2′s and backcrosses) have been used to investigate the genetics of dry matter yield in a population of Lolium perenne. Two trial designs, short, individual plant drills and larger sown plots were used at each of two sites in the U.K., at Aberystwyth and Birmingham. The families studied correspond to those raised as spaced plants and reported in the first paper in this series. In contrast to the spaced plants trial, the drill trial resulted in considerable epistasis as well as additivity and dominance variation whilst the plot trial failed to identify any significant genetic variation. There was considerable G × E interaction over sites and harvests, particularly in the amount of dominance. The latter showed a clear linear relationship with mean performance at both sites, the dominance ratio being higher in the poorest environments. This is largely due to the additive variance decreasing in the poor environments rather than the dominance being higher. The implications of these results to grass breeding are discussed.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 111 (1993), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In order to establish an appropriate breeding programme, the reproductive system of a population of Lolium rigidum Gaud, from Sardinia was analyzed to determine the mechanisms operational in this species. A dual approach was adopted of genotype assay, exploiting the polymorphism at the Pgi-2 locus, and an assessment of the effects of inbreeding on agronomic characters.The statistical analysis showed highly significant differences for the number of plants due to selfing among the 35 progenies obtained by controlled crossing of genotypes homozygous for different alleles at the Pgi-2 locus. The greatest inbreeding effect was on dry matter yield. Although at a bulk population level the species is outbreeding, highly self-compatible plants can be found within this naturally-derived population.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 98 (1987), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Crossing bags manufactured from three grades of polyester and three types of paper bags were tested for their permeability to grass pollen. Bags were placed on L. perenne plants of known PGI isozyme genotype. Any pollen passing through the crossing bag and pollinating the plant was detected by assaying its progeny for PGI isozymes. The results obtained allowed differentiation of the various materials for their suitability for pollen proofing.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 101 (1988), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A series of lines, differing in isozyme genotype, were constructed by selection from within three cultivars of Lolium perenne. Field assessment of the progeny of these selected line? revealed variation in yield and timing of inflorescence emergence. This variation between lines from within a cultivar could not be accounted for by the differing levels of homozygosity attained. In some instances differences in yield were associated with homozygosity for specific alleles at the PGI/2 locus, background genotype was however, of major importance. The variation identified could be attributable to founder effects or possible linkage of quantitative trail loci to the PGI/2 locus.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Chromosoma 67 (1978), S. 87-96 
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Eighteen plants displaying varying degrees of asynapsis ranging from weak to very strong were found among four out of six populations of Lolium perenne L. (2n=14) which had been subjected to three cycles of directional phenotypic selection for productivity of green material. No plants were found displaying univalents in the original generation but the incidence increased with cycles of selection, indicating the genetic control and differential distribution of asynaptic genes among these populations. — The analysis of univalents and chiasma frequency of pollen mother cells (PMC) of six partially asynaptic plants chosen for detailed study revealed that univalents occurred throughout all PMC chiasma classes irrespective of chiasma frequency, but the higher the chiasma frequency of any PMC the less the likelihood of univalents occurring. The relationship between chiasma frequency and univalent frequency per PMC per plant was negative. — Mean chiasma frequency per bivalent increased for the asynaptic cells in comparison with the normal in both the weak and medium asynaptic groups which was explained by the availability of additional chiasmata for redistribution.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 70 (1985), S. 48-51 
    ISSN: 1432-2242
    Keywords: Lolium perenne ; Selection ; Multiplication ; Stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The performance of three experimental cultivars of Lolium perenne selected for yield or water soluble carbohydrate content was monitored over four generations of seed multiplication under relaxed selection. In each variety the selected trait regressed towards that of the base population from which the selection line derived. This could be accounted for by residual genetic variation within the lines for the selected trait, and in some instances, by association of this variation with the fitness character, seed numbers produced. These results emphasize the need for practical breeding programmes to consider the nature of the gene action controlling the selected trait, if additive, directional selection should be effective in increasing the expression of the character. Where ambidirectional dominance and epistasis are important, consideration should be given to means of achieving reassortment of the controlling genes prior to selection.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 75 (1987), S. 66-75 
    ISSN: 1432-2242
    Keywords: Lolium ; F1 hybrids ; Recombinant inbred lines ; Triple test crosses ; Genotype by environment interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genetical control of heading date and dry matter production in an ‘F2’ population of Lolium perenne are presented from analyses of triple-test-crosses and basic generations. The data is derived from spaced plant trials at sites in the United Kingdom (UK) and Italy in different years. Despite the wide initial cross between UK bred material and an Italian accession, there was no significant evidence for epistasis, while additive and dominance variation were generally present with partial to complete dominance for all traits. Linear regression onto the environmental means accounted for all the GxE variation for dry matter production in the establishment and aftermath cuts, but not for heading date or the hay cut. The b values measuring responsiveness to the environment were clearly heritable and showed partial dominance. Predictions of the likely performance of recombinant inbred lines and second cycle hybrids were sufficiently promising to support further investigation of these approaches to breeding in this crop.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 99 (1999), S. 445-452 
    ISSN: 1432-2242
    Keywords: Key words Genetic mapping ; AFLP ; Lolium perenne ; Plant breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  AFLP markers have been successfully employed for the development of a high-density linkage map of ryegrass (Lolium perenne L.) using a progeny set of 95 plants from a testcross involving a doubled-haploid tester. This genetic map covered 930 cM in seven linkage groups and was based on 463 amplified fragment length polymorphism (AFLP) markers using 17 primer pairs, three isozymes and five EST markers. The average density of markers was approximately 1 per 2.0 cM. However, strong clustering of AFLP markers was observed at putative centromeric regions. Around these regions, 272 markers covered about 137 cM whereas the remaining 199 markers covered approximately 793 cM. Most genetic distances between consecutive pairs of markers were smaller than 20 cM except for five gaps on groups A, C, D, F and G. A skeletal map with a uniform distribution of markers can be extracted from this high-density map, and can be applied to detect and map QTLs. We report here the application of AFLP markers to genome mapping, in Lolium as a prelude to quantitative trait locus (QTL) identification for diverse agronomic traits in ryegrass and for marker-assisted plant breeding.
    Type of Medium: Electronic Resource
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