ISSN:
1573-5036
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary Azotobacter bacteriophages were studied using liquid as well as solid enrichment cultures. The study included 16 samples representing the soils of Ruzyně, Prague. The effect of long-term application of mineral fertilizers (N, P, K) and organic manure (straw enriched by inorganic nitrogen) to the soil on Azotobacter and their phages was investigated. Liquid enrichments yielded phages in fairly high titers, and phages were found in most of the soils while solid ones only gave low titers in occasional soils. On the basis of plaque morphology 4 different phage types were obtained. Electron micrographs showed that the phages belong to the groups with long non-contractile, contractile, and short contractile tails. Host specificity assay showed that the majority of the strains of A. chroococcum, A. vinelandii, and one strain of A. beijerinckii were lysed by phage, while none of A. macrocytogenes, A. agilis and A. insignis strains were lysed. Phage inactivation and neutralization was carried out with homologous antisera. re]19741128
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00011701
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