ISSN:
1617-4623
Keywords:
Nitrate reductase
;
Revertants
;
Genetic analysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Spontaneous revertants of nitrate reductase (NR)-less mutants were isolated by screening for nitrate utilization in diploid NR− protoplast cultures of Nicotiana plumbaginifolia. The revertants contained in vivo NR activity in the case of apoenzyme mutants (nia) as well as of a cofactor-deficient (cnx) mutant. Revertants of the NIA type proved to be tetraploid, and genetic analysis showed that only one out of the four NR structural genes had reverted to a functional allele.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00331656
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