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  • 1
    Electronic Resource
    Electronic Resource
    Ultrastructure of epithelial cells in the fundus glands of the mouse gastric mucosa
    Amsterdam : Elsevier
    Journal of Ultrasructure Research 3 (1959), S. 74-83 
    Details
    ISSN: 0022-5320
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0022-5320(59)80017-4
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  • 2
    Electronic Resource
    Electronic Resource
    Quantitative electron microscopical studies onin Vitro incubated rabbit gallbladder epithelium (1977)
    Springer
    The journal of membrane biology 37 (1977), S. 45-61 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Pieces of rabbit gallbladders were incubated in vitro for 1 hr in Ringer's solution at 37° (“transporting epithelium”), or in Ringer's containing 1mm ouabain (“inhibited epithelium”). The tissues were fixed in 2% glutaraldehyde, postfixed in OsO4 and embedded in Epon. Stereological analysis was carried out on electron micrographs; with this type of analysis it is possible to obtain quantitative data for the three-dimensional structure of the tissue. The following data were obtained for crest transporting epithelium (crypt epithelium in parentheses): Epithelial height, 28 (18) μm; nucleus, 16.9 (18.7) % of cell volume; mitochondria, 18.7 (14.1) % of cytoplasmic volume; paracellular channels, 28.3 (11.0)% of epithelial volume; lateral plasma membrane, 4.1 (2.9) m2/cm3, and apical membrane, 0.5 (0.6) m2/cm3 of cell volume. The paracellular channels appeared as interconnected tissue sheets of a highly varying width. They were broader in the basal portion of the epithelium than in the apical portion; however, immediately above the basement membrane the channels became very narrow. The arithmetic mean width of the channels was 0.9 (0.2) μm and their length was in the range of 40–120 μm. Our stereological data seem compatible with the standing gradient model for water transport. The ouabain inhibited cells were swollen with collapsed paracellular channels. It cannot be ruled out that volume changes occurred in the cells and the paracellular channels during the processing of the tissue. However, qualitatively the same morphology was observed by light microscopy of transporting and inhibited gallbladder epithelium fixed by freeze-drying and embedded in Epon. This constitutes a strong indication of the presence of such channels in the living, in vitro transporting rabbit gallbladder epithelium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01940923
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  • 3
    Electronic Resource
    Electronic Resource
    How to avoid maternal cannibalism after neonatal surgery in rats (1980)
    Springer
    Cellular and molecular life sciences 36 (1980), S. 1295-1296 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Following surgery newborn rats are often eaten by their mothers. This can be avoided if the wound is closed carefully, the blood removed from the skin, and the wound area covered by a plastic film.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01969597
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Bile salt-stimulated lipase (BSSL) distribution in rat, mouse and transgenic mouse expressing human BSSL (1998)
    Springer
    Histochemistry and cell biology 110 (1998), S. 367-376 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  In some species, including man and mouse, bile salt-stimulated lipase (BSSL) in milk catalyzes the hydrolysis of triacylglycerides into glycerol and free fatty acids, a reaction that is of particular importance during suckling. The enzyme is also secreted by the pancreas (referred to as carboxyl-ester hydrolase, CEH). We wished to localize sources and storage sites for BSSL/CEH in rats, in wild-type mice, and in transgenic mice producing recombinant human BSSL in milk. Immunoreactivity against several BSSL fragments was strong in the pancreatic acinar cells and moderate in the absorptive cells of the small intestine and in salivary duct cells of the mice, as well as in rats. Sections from lactating mammary glands of mouse, but not rat, also showed immunoreactivity for BSSL; the signal was strongest in the transgenic mice. Radioactive riboprobes for BSSL mRNA hybridized on sections of rat and mouse pancreatic acinar cells, and mouse mammary glands (both wild-type and transgenic). Using RT-PCR, it was possible to amplify BSSL mRNA from wild-type mouse pancreas and mammary gland, from rat submandibular glands, and, in a few cases, from rat liver. In transgenic mice, the BSSL mRNA was highly expressed only in lactating mammary gland, but could be detected in a few other organs as well.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050297
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  • 5
    Electronic Resource
    Electronic Resource
    Acid suppression and gastric mucosal cell biology (1994)
    Springer
    Digestive diseases and sciences 39 (1994), S. 1843-1852 
    Details
    ISSN: 1573-2568
    Keywords: acid suppression ; enterochromaffin-like cells ; gastric carcinoids ; gastric mucosa ; intestinal metaplasia ; multiple endocrine neoplasia syndrome ; unscheduled DNA synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This review examines recent concepts of gastric mucosal cell biology in relation to acid inhibition. Powerful acid-inhibitory drugs have been associated with the production of enterochromaffin-like (ECL) cell proliferation and the induction of ECL-cell carcinoids in rats. The ECL-cell lineage and its renewal is discussed, and the factors that regulate ECL-cell proliferation are reviewed. Current methods in use for assessing genotoxicity in gastric mucosa are scrutinized; the much discussed claim that antisecretory drugs induce unscheduled DNA synthesis is examined, and the methodology that is the basis for these claims is found defective and wanting. The nature of ECL-cell proliferation in rats receiving lifelong treatment with H2-receptor antagonists or acid pump inhibitors is explored, and their relationship to ECL-cell proliferation and ECL-cell carcinoids discussed. It is concluded that aged rats are very prone to developing endocrine proliferations, and this may be related to the multiple endocrine neoplasia syndrome found in humans. There is no evidence at present that long-term antisecretory therapy causes significant ECL-cell proliferation in humans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02088113
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Acid suppression and gastric mucosal cell biology (1995)
    Springer
    Digestive diseases and sciences 40 (1995), S. 121S 
    Details
    ISSN: 1573-2568
    Keywords: acid suppression ; enterochromaffin-like cells ; gastric carcinoids ; gastric mucosa ; intestinal metaplasia ; multiple endocrine neoplasia syndrome ; unscheduled DNA synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This review examines recent concepts of gastric mucosal cell biology in relation to acid inhibition. Powerful acid-inhibitory drugs have been associated with the production of enterochromaffin-like (ECL) cell proliferation and the induction of ECL cell carcinoids in rats. The ECL cell lineage and its renewal is discussed, and the factors that regulate ECL-cell proliferation are reviewed. Current methods in use for assessing genotoxicity in gastric mucosa are scrutinized; the much discussed claim that antisecretory drugs induce unscheduled DNA synthesis is examined, and the methodology that is the basis for these claims is found defective and wanting. The nature of ECL cell proliferation in rats receiving lifelong treatment with H2-receptor antagonists or acid pump inhibitors is explored, and their relationship to ECL cell proliferation and ECL cell carcinoids discussed. It is concluded that aged rats are very prone to developing endocrine proliferations, and this may be related to the multiple endocrine neoplasia syndrome found in humans. There is no evidence at present that long-term antisecretory therapy causes significant ECL cell proliferation in humans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02214875
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Ultrastructure of the freeze-dried mouse thyroid gland (1963)
    Springer
    Cell & tissue research 59 (1963), S. 467-478 
    Details
    ISSN: 1432-0878
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Mouse thyroids were preserved for electron microscopy by means of freeze-drying. The tissue specimens were frozen in liquid isopentane, dried at a temperature of −79° C to a pressure of 4×10-5 mm Hg, stained in vacuo with osmium tetroxide vapour at room temperature, and embedded in vacuo in Epon or Vestopal W. The ultrastructure of the freeze-dried thyroid gland was found to be fundamentally similar to that observed after “ordinary” chemical fixation. However, some differences were noticed. Thus the plasma membrane of the lateral cell surfaces appeared to be asymmetrical, its inner dense layer being thicker than the outer one. The mitochondria had a finely undulating contour and a matrix of rather high density. The mitochondrial outer and inner membranes appeared as five-layered structures and comprised three dense and two less dense layers. Small dense granules with an undulating outline and larger, less dense granules with a distinct surface membrane were observed. The intra-cisternal space had a lower density than the extra-cisternal space. 150 Å particles occurred very sparsely. The karyoplasm had a low density and the particulate component of the nucleus was rather scanty. By means of pores in the nuclear envelope the karyoplasm appeared to communicate with the extra-cisternal space. No basement membranes were observed. The appearance of myelin sheaths in freeze-dried specimens seemed to agree well with the picture after chemical fixation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00368721
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