ISSN:
1573-4943
Keywords:
G proteins
;
transducin
;
biotinylation
;
reconstitution
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract A method is described for modifying G proteins with biotin. With transducin, better results were obtained with the amino group-specific derivative BXNHS (biotinyl-ε-aminocaproic acid N-hydroxysuccinimide ester) as compared to the -SH-group specific reagent MBB (maleimidobutyrylbiocytin). Modification occurred under conditions preserving functional activity: Interaction of the biotinylated transducin with rod outer segment membranes was shown by its light-dependent association and by a GTPγS-binding assay. G0 from bovine brain was also biotinylated under conditions preserving its activity. Biotinyl-α0 was shown to bind to a streptavidin Sepharose matrix. Biotinyl-G proteins, therefore, are proposed as tools for extracting proteins (receptors and effector systems), which interact under specific conditions with G proteins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01025631
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