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  • 1
    Electronic Resource
    Electronic Resource
    Patents and royalties (1997)
    [s.l.] : Macmillan Magazines Ltd.
    Nature 387 (1997), S. 546-546 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SirPhilippe Ducor points out in his Commentary (Nature 387, 13–14; 1997) that due to “the wise licensing policy of non-exclusivity and modest pricing adopted by the owner (Stanford University), [recombinant DNA] technology ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/42345
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  • 2
    Electronic Resource
    Electronic Resource
    Processing of chloroplast ribosomal RNA transcripts in Euglena gracilis bacillaris (1989)
    Springer
    Current genetics 15 (1989), S. 363-370 
    Details
    ISSN: 1432-0983
    Keywords: Euglena gracilis ; rRNA transcription ; Chloroplasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ribosomal RNA operons (rrn operons) of Euglena gracilis chloroplasts contain genes for (in order) 16S rRNA, tRNAIle, tRNAAla, 23S rRNA and 5S rRNA. Major sites of cleavage of the primary rrn transcript were identified by Northern blot hybridization and S1-mapping. The presumptive termini of all of the mature products have now been identified. During initial processing in the chloroplast, the primary transcript is cleaved between the two tRNAs and between the 23S and 5S rRNAs so as to separate the sequences found in the different mature rRNAs. Subsequently the tRNAs are separated from the rRNAs, further trimming provides the remaining proper ends, and the 3′-ends of the tRNAs are added.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419917
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  • 3
    Electronic Resource
    Electronic Resource
    Initiation of rrn transcription in chloroplasts of Euglena gracilis bacillaris (1988)
    Springer
    Current genetics 14 (1988), S. 493-500 
    Details
    ISSN: 1432-0983
    Keywords: Euglena gracilis ; rRNA transcription ; Chloroplasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The site of initiation of chloroplast rRNA synthesis was determined by Sl-mapping and by sequencing primary rRNA transcripts specifically labeled at their 5′-end. Transcription initiates at a single site 53 nucleotides upstream of the 5'-end of the mature 16S rRNA under all growth conditions examined. The initiation site is within a DNA sequence that is highly homologous to and probably derived from a tRNA gene-region located elsewhere in the chloroplast genome. A nearly identical sequence (102 of 103 nucleotides) is present near the replication origin. The near identity of the two sequences suggests a common mode for control of transcription of the rRNA genes and initiation of chloroplast DNA replication. The related sequence in the tRNA gene-region does not appear to serve as a transcript initiation site.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00521275
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  • 4
    Electronic Resource
    Electronic Resource
    Erratum (1989)
    Springer
    Current genetics 16 (1989), S. 129-129 
    Details
    ISSN: 1432-0983
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393407
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Structure and rearrangements of rRNA genes in chloroplast DNA in two strains of Euglena gracilis (1984)
    Springer
    Plant molecular biology 3 (1984), S. 127-136 
    Details
    ISSN: 1573-5028
    Keywords: Euglena gracilis ; strain bacillaris ; strain Z-S ; chloroplast ; rRNA genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The organisation of the rRNA genes in the chloroplast genomes of two strains of Euglena gracilis were analyzed and compared. It was previously shown that the bacillaris strain contains three complete rrn (rRNA) operons (7) and that the Z-S strain contains one operon (21). Using heteroduplex analysis it was found that the bacillaris strain contains, apart from the three complete rrn operons, an extra 16S rRNA gene, an extra partial 23S rRNA gene sequence and an inverted duplication of a stretch within the 5S–16S spacer. In addition a short (〈100 bp) inverted repeat sequence (13) which forms a stem/loop structure in single-stranded cpDNA was located between the 3′-end of the extra 16S rRNA gene and the partial 23 S rRNA sequence. The Z-S strain differs from the bacillaris strain by a deletion of two units of the complete rrn operons. The region upstream of the single complete rrn operon, including the inverted repeats, the partial 23S and the extra 16S rRNA sequences is identical with the bacillaris strain. The only non-homology found in heteroduplexes between the SalI fragments of B of the two strains is the deletion-insertion loop which represents the two rrn operons. A small deletion loop was found occasionally in hetero-and in homoduplexes of both strands in the region of variable size. Apart from the deletion/insertion of two rrn operons the two genomes appear to be colinear as can be seen from partial denaturation mapping. The organisation of the rRNA genes of the two strains is compared with those of the Z strain and the bacillaris-ATCC strain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016060
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  • 6
    Electronic Resource
    Electronic Resource
    A map of specific cleavage sites and tRNA genes in the chloroplast genome of Euglena gracilis bacillaris (1981)
    Springer
    Molecular genetics and genomics 181 (1981), S. 296-305 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A map showing locations of 22 of the 30 endonuclease EcoRI cleavage sites and 54 additional sites for eight other restriction endonucleases is presented. The regions of chloroplast DNA that hybridize with chloroplast tRNA are also shown.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425601
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  • 7
    Electronic Resource
    Electronic Resource
    Sequence and evolution of the regions between the rrn operons in the chloroplast genome of Euglena gracilis bacillaris (1984)
    Springer
    Molecular genetics and genomics 194 (1984), S. 432-443 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The rRNA genes are arranged in three sequential operons preceded by a fourth partial operon. Part or all of a 1462 nucleotide sequence extending from within the 3′-end of the 23S rRNA gene, across the 5S rRNA gene and a presumptive transcription terminator, to within the first structural gene (for 16S rRNA) of the rrn operon was determined for each region between operons. Homologies of the 3′-end of the 23S rRNA gene with the 4.5S rRNA genes of higher plant chloroplasts, and of the 5S rRNA gene with other 5S rRNA genes were examined. The region preceding the 16S rRNA gene, which is expected to contain sites for initiation and regulation of rrn transcription, includes a 305 base-pair sequence with substantial homology with structural genes elsewhere in the chloroplast genome. The homologies suggest that this portion of the leader evolved from copies of parts of the structural genes which had been inserted before the 16S rRNA genes. Thus the chloroplast rrn leader may provide a unique opportunity to study how a regulatory sequence evolved from well-defined structural genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425555
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  • 8
    Electronic Resource
    Electronic Resource
    The glutamate dehydrogenase structural gene of Escherichin coli (1990)
    Springer
    Molecular genetics and genomics 223 (1990), S. 508-512 
    Details
    ISSN: 1617-4623
    Keywords: Escherichia coli ; Glutamate dehydrogenase ; gdhA gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The glutamate dehydrogenase structural gene, gdhA, was mapped at 38.6 min on the genetic map and at 1860 kb on the physical map. A detailed map of this region is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264460
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  • 9
    Electronic Resource
    Electronic Resource
    Organization of the chloroplast ribosomal RNA genes of Euglena gracilis bacillaris (1979)
    Springer
    Molecular genetics and genomics 174 (1979), S. 1-10 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The order of eight of the 29 endonuclease EcoRI-generated fragments of chloroplast DNA was determined. Three sets of rRNA genes aligned sequentially in the same orientation form part of this region. The repeated sets differ in the length and sequence of the spacers among themselves and with the rRNA genes of E. gracilis strain Z.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00433298
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