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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    International Journal of Food Microbiology 21 (1994), S. 69-77 
    ISSN: 0168-1605
    Keywords: Clonal concept ; Electrotyping ; Plasmid profiling ; Ribotyping ; Salmonella enteritidis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 146 (1997), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: All strains of Salmonella enterica investigated were found to carry the Salmonella enterotoxin gene (stn) as determined by PCR and hybridization studies. However, when using CHO-K1 cells for testing the toxicity of the strains, not all strains showed a toxic effect (cell elongation) on the cells or did so only at a low level. The cultivation of Salmonella in contact with epithelial cells (IEC-6) led to an increase in the production of toxin. The stn gene expression was detectable with the help of the RT-PCR after 3 h of incubation. The RNA of the strains was isolated, transcribed into cDNA (with MMLV-reverse transcriptase) and amplified using PCR. The PCR products were separated electrophoretically using a polyacrylamide gel and detected by silver staining.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz 42 (1999), S. 26-34 
    ISSN: 1437-1588
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zusammenfassung In den letzten anderthalb Jahren hat die Diskussion um den Einsatz antimikrobiell wirksamer Substanzen in der Veterinärmedizin wesentlich an Intensität zugenommen. Die Gründe dafür liegen im Anstieg der Resistenzraten bei bakteriellen Krankheitserregern, sowohl im human- als auch im veterinärmedizinischen Bereich. Speziell in der Agrarwirtschaft und beim landwirtschaftlichen Nutztier jedoch gibt es Bereiche, die außerhalb der klassischen Therapieformen liegen. Dazu gehören vor allem rein prophylaktische Maßnahmen mit teilweise fragwürdigen Wirkstoffkombinationen und Dosierungen sowie der nutritive Einsatz antimikrobiell wirksamer Substanzen zu wachstumsfördernden Zwecken. Im vorliegenden Artikel wird versucht, umfangreiche Expertentagungen und Berichte zum Thema der Resistenzentwicklung gegenüber antimikrobiell wirksamen Substanzen zusammenzufassen und den momentanen Stand der Diskussion im Bereich der Veterinärmedizin wiederzugeben. Dem BgVV erscheinen die momentan vorhandenen Gesetzesgrundlagen zur Regelung und Zulassung des Gebrauchs von antimikrobiell wirksamen Substanzen ausreichend. Dabei ist es gleich, ob sie als Arzneimittel oder zu wachstumsfördernden Zwecken eingesetzt werden. Wichtig hierbei ist, daß diese Regelungen befolgt und durch entsprechende Überwachungsmaßnahmen umgesetzt und kontrolliert werden. Die Agrarwirtschaft sollte sich darum bemühen, den Antibiotikaeinsatz in Zukunft auf die Therapie der Tiere zu beschränken und andere Anwendungen durch bereits existierende oder neu zu schaffende alternative Maßnahmen (Hygiene, Management, Impfprogramme, Probiotika, Züchtung resistenter Rassen etc.) zu ersetzen.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1570-7458
    Keywords: Scrobipalpopsis solanivora ; Lepidoptera ; Gelechiidae ; sex pheromone ; (E)-3-dodecenyl acetate ; (Z)-3-dodecenyl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé S. solanivora est l'un des plus importants lépidoptères détruisant les pommes de terre en Amérique Centrale. L'analyse par chromatographie en phase gazeuse (GC) des produits obtenus par lavage de l'ovipositeur de femelles vierges, associée à des électro-anténnogrammes (EAG) a révélé un composé actif unique en EAG. Les durées de rétention pour ce constituant correspondaient à celles des acétates monoinsaturés à 12 carbones, et les analyses sur colonne GC liées avec une phase liquide cristal ont montré un pic principal correspondant à l'acétate (E)-3-dodécénul et un pic secondaire, correspondant à l'isomère Z, et au niveau de 2,5% du pic principal. L'acétate dodécyl a aussi été décelé en quantités variables, approximativement 10% du pic principal. Dans la nature, des pièges contenant de l'acétate (E)-3-dodécényl, seul ou mélangé avec 1 ou 2% de l'isomère Z, ont capturé autant des mâles de S. solanivora que des pièges contenant des femelles vierges, et certaines indications montrent que l'addition de 5% de l'isomère Z réduit les captures.
    Notes: Abstract The sex pheromone produced by virgin female S. solanivora moths has been shown to contain (E)-3-dodecenyl acetate with approximately 2% of the Z isomer by electroantennography and gas chromatography. In the field, traps baited with (E)-3-dodecenyl acetate alone or in combination with 1% or 2% of the Z isomer caught at least as many male S. solanivora moths as those baited with virgin female moths, and there was some evidence that addition of 5% of the Z isomer reduced catches.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-7284
    Keywords: Multi-resistance ; Plasmid typing ; Phage type 204c ; Salmonella typhimurium ; Sewage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Salmonella contamination of North Sea water was detected for the first time in 1988 in Germany during routine examinations of bathing areas. Since then, subsequent isolations along the coast have been reported regularly. To define the source of contamination, strains isolated from seawater and rivers were studied by molecular marker methods. Their properties were compared with those of strains originating from possible sources of contamination such as humans, cattle, and sewage treatment plant water. Plasmid profile analysis of whole bacterial populations and the determination of antibiotic resistance patterns demonstrated, that contamination through the surrounding cattle industry could be excluded. Cattle isolates belonged to a widespread clone of phage type 204c which was multiresistant and exhibited an unique plasmid pattern which was never found in sea water isolates. Outer membrane protein and lipopolysaccharide analysis failed to demonstrate differences among theSalmonella populations and proved in this case insufficient for molecular marker discrimination.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    European journal of epidemiology 10 (1994), S. 645-648 
    ISSN: 1573-7284
    Keywords: Phage typing ; Phage type 4 (PT4) ; Salmonella enteritidis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In order to monitor the epidemiological situation ofS. enteritidis in Germany, in 1990–91 1138 isolates from more than 180 locations in West Germany were phage typed. 1124 strains (98.8%) from all sources were typeable, belonging to 21 different phage types (PT). PT4 strains were isolated most frequently (70.8%). In addition, PT7, 25, 34 and 8 were of epidemiological relevance with incidences of 7.2 to 4.5%. The comparison of data shows that in Germany, like in other parts of Europe, PT4 predominates. This phage type is, however, infrequent in North America, where PT8 has the highest incidence.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Chichester, West Sussex : Wiley-Blackwell
    Mathematical Methods in the Applied Sciences 19 (1996), S. 1415-1431 
    ISSN: 0170-4214
    Keywords: Engineering ; Numerical Methods and Modeling
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics
    Notes: The Dirac equation with a vector potential is considered using a biquaternionic formalism and boundary integral representations for its solutions are obtained. In order to characterize these solutions by a property of local approximability by linearization the corresponding notion of biquaternionic differentiability and its generalization in the sense of Bers (adapted to the spatial case) are given.
    Type of Medium: Electronic Resource
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