ISSN:
0749-503X
Keywords:
vandate
;
mitochondria
;
H+ ATPase
;
S. cerevisiae
;
Life and Medical Sciences
;
Genetics
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
The effects of vandate on mitochondrial respiration and H+ ATPase activity in Saccharomyces cerevisiae were studied. A 50% inhibition of oxygen uptake in isolated mitochondria was produced by 4·4 mM-V2O5. Activity of H+ ATPase in whole mitochondria was inhibited by 50% by 5·5 μM-V2O5, in submitochondrial particles by 55 μM-V2O5; and in the chloroform-released H+ ATPase by 0·5 mM-V2O5. Vandate was also found to relieve growth inhibition caused by the mitochondrial H+ ATPase inhibitors NN′-decyclohexylcarbodiimide and oligomycin. These results imply that vanadate could affect mitochondrial respiration by interacting with the H+ ATPase in S. cerevisiae.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/yea.320050109
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