ISSN:
1617-4623
Keywords:
ndh gene
;
Intron
;
NADH dehydrogenase
;
Transcription
;
Tobacco
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Sequences (ndhA-F) homologous to human mitochondrial genes for components of the respiratory chain NADH dehydrogenase have been found in the chloroplast DnA of tobacco. The ndhA, D, E and F sequences corresponding to the mitochondrial URF1, 4, 4L and 5 are located in the small single copy region, the ndhB sequence corresponding to URF2 in the inverted repeat and the ndhC sequence corresponding to URF3 in the large single copy region of the chloroplast DNA. Northern blot hybridization revealed that all six ndh sequences are actively expressed in the chloroplasts. The ndhA and ndhB sequences contain single introns and the splice sites of their pre-mRNAs were determined by reverse transcription analysis. These findings suggest that potential components of an NADH dehydrogenase are synthesized in the chloroplasts.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00327187
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