ISSN:
1460-9568
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
The γ-aminobutyric acid (GABA) receptor ρ subunits recently cloned from rat and human retina are thought to form GABA receptor channels belonging to a pharmacologically distinct receptor class, termed GABAC. In this work we have examined the distribution of ρ1, ρ2 and ρ3 subunits, and found expression of all three transcripts in several regions of the rat nervous system. In situ hybridization revealed expression of ρ2 in the adult rat retina and some other parts of the visual pathways. A high local ρ2 expression was seen in the superficial grey layer of the superior colliculus, and in the dorsal lateral geniculate nucleus. Expression was also detected in the 6th layer of visual cortex and in the CA1 pyramidal cell layer of hippocampus. With reverse transcriptase–polymerase chain reaction, expression of ρ1 was mainly seen in the adult rat retina and dorsal root ganglia, as well as, at a significantly lower level, in the superior colliculus, hippocampus, brain stem, thalamus, postnatal day 8 (P8) superior colliculus and P8 hippocampus. Expression pattern of ρ3 mRNA was clearly different from that of ρ1 and ρ2, being strongest in the hippocampus, and significantly lower in the retina, dorsal root ganglia and cortex. No ρ3 expression was observed in adult or P8 superior colliculus or in P8 hippocampus.The present results clearly demonstrate that expression of GABA receptor ρ subunits is not restricted to the retina, but significant expression can also be detected in many other brain regions, especially in those belonging to the visual pathways. The expression pattern of the ρ subunits may be helpful in solving the functional significance of the receptors formed from these subunits.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1046/j.1460-9568.1998.00023.x
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