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  • 1
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The crystallization and preliminary crystallographic study of phycocyanin from the thermophilic cyanobacterium Synechococcus elongatus is reported. Phycocyanin is composed of α- and β-subunits consisting of 162 and 172 amino-acid residues, respectively. These associate to form an αβ heterodimer, which further associates to give a ring-shaped trimer (αβ)3. Two trimers bind head-to-head to form a hexamer (αβ)6. Phycocyanin crystals have been obtained by the sitting-drop vapour-diffusion method with a precipitant solution containing 30%(w/v) PEG 4000 and 100 mM MES pH 7.5–8.0. Using synchrotron radiation, the crystals diffract to 2.0 Å resolution. They belong to the trigonal space group R32, with unit-cell parameters a = b = 186.75 (3), c = 59.75 (4) Å, α = β = 90, γ = 120°. Assuming that the crystallographic triad is identical to the threefold axis of the hexamer and with three (αβ)6 molecules in a unit cell, the calculated molar volume (VM) is 2.64 Å3 Da−1. This value corresponds to a solvent content of approximately 53%, with one αβ heterodimer occupying the asymmetric unit.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Molecular and cellular biochemistry 199 (1999), S. 57-62 
    ISSN: 1573-4919
    Keywords: 5-hydroxytryptamine (5-HT) receptor subtype ; 5-HT1B ; 5-HT2A ; quantitative reverse transcription-polymerase chain reaction (RT-PCR) ; artery ; vein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Expression of two subtypes of 5-hydroxytryptamine (5-HT) receptors, 5-HT1B and 5-HT2A, was investigated in normal rat arteries (abdominal aorta) and veins (inferior vena cava) by the quantitative reverse transcription-polymerase chain reaction (RT-PCR). The arterial 5-HT2A receptor mRNA level in artery was 25-fold (p 〈 0.01) higher than the venous level, and the arterial 5-HT1B receptor mRNA level was at least 7000-fold (p 〈 0.005) higher the venous level.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-4919
    Keywords: space flight ; osteoblast ; PDGF-β receptor ; EGF receptor ; Shc ; c-fox
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract A number of studies have indicated that microgravity induces osteopenia and modulates functions of mammalian cells. However, the molecular mechanisms underlying these effects of microgravity are still unknown. Rat osteoblasts were cultured for 4 and 5 days during Shuttle-Spacelab flight, and fixed by guanidine isothiocyanate solution on board after treatment with 1α, 25 (OH)2 vitamin D3. The mRNA levels for platelet-derived growth factor (PDGF)-β receptor, epidermal growth factor (EGF) receptor, the growth factor receptor adaptor protein Shc, and c-fos were determined using the method of quantitative reverse transcription-polymerase chain reaction. The mRNA levels for EGF receptor were not altered by microgravity. However, the mRNA levels for PDGF-β receptor, Shc, and c-fos were decreased to 62, 55 and 25% on the 4th day of flight, and 47, 40, and 43% on the 5th day, respectively, as compared to the corresponding ground controls. Expression of the growth factor receptor and the receptor adaptor protein was modulated in rat osteoblasts during spaceflight. Data suggest that signal transduction via growth factor receptors in rat osteoblasts is impaired by microgravity. Dysfunction of osteoblasts might be involved in spaceflight-induced osteopenia.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Photosynthesis research 1 (1980), S. 149-162 
    ISSN: 1573-5079
    Keywords: blue-green algae ; cytochrome 553 ; electron transport ; photosynthesis ; plastoquinone ; respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The role of plastoquinone in a thermophilic blue-green alga, Shynechococcus sp., was studied by measuring reduction kinetics of cytochrome 553 which was oxidized with red flash preferentially exciting photosystem I. Sensitivity of the cytochrome reduction to DBMIB Abbreviations: DCMU = 3-(3,4-dichlorophenyl)-1,1-dimethylurea; DBMIB = 2,5-dib romo-3-methyl-6-isopropyl-p-benzoquinone; HOQNO = 2-n-heptyl-4-hydroxyquinoline-N-oxide indicates that cytochrome 553 accepts electrons from reduced plastoquinone. Plastoquinone is in turn reduced in cells without electrons from photosystem II, since DCMU Abbreviations: DCMU = 3-(3,4-dichlorophenyl)-1,1-dimethylurea; DBMIB = 2,5-dib romo-3-methyl-6-isopropyl-p-benzoquinone; HOQNO = 2-n-heptyl-4-hydroxyquinoline-N-oxide , which inhibited methyl viologen photoreduction more strongly than DBMIB, failed to affect the cytochrome reduction. Participation of cyclic electron transport around photosystem I in cytochrome reduction in the presence of DCMU was excluded, because methyl viologen and antimycin A had no effect on the cytochrome kinetics. On the other hand, electron donation from endogenous substrates to plastoquinone was suggested from decreases in rate of the cytochrome reduction by dark starvation of cells and also from restoration of fast reduction kinetics by the addition of exogenous substrates to or by reillumination of starved cells. KCN, which completely suppressed respiratory O2-uptake, induced a marked acceleration of the cytochrome reduction in starved cells. The poison was less or not effective in stimulating the cytochrome reduction in more extensively starved or reilluminated cells. Results indicate that plastoquinone is functioning not only in the photosynthetic but also in the respiratory electron transport chain, thereby forming a common link between the two energy conservation systems of the blue-green alga.
    Type of Medium: Electronic Resource
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