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  • 1
    Electronic Resource
    Electronic Resource
    Cryopreservation of Chrysanthemum cinerariaefolium Vis. cells and its impact on their pyrethrin biosynthesis ability (1997)
    Springer
    Plant cell reports 17 (1997), S. 60-64 
    Details
    ISSN: 1432-203X
    Keywords: Key words Cryopreservation ; Chrysanthemum cinerariaefolium ; Callus cultures ; Pyrethrins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An efficient protocol for cryogenic storage of high-pyrethrin-producing cell lines of Chrysanthemum cinerariaefolium is described. Optimal survival (92%) was obtained with cells precultured in 1/2 Murashige and Skoog nutrient medium containing 180 g ⋅ l–1 sucrose for 30 days, then incubated in the same medium in the presence of 5% DMSO for 1 h in an ice bath, cooled slowly to –20°C and immersed for 30 min in liquid nitrogen. After cryopreservation, the cells conserved the same growth pattern, but displayed different biochemical properties. The subculture derived from the thawed cells was characterized by a lower chlorophyll content and a higher pyrethrin biosynthesis ability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050352
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Rapid mass propagation of Chrysanthemum cinerariaefolium Vis. by callus culture and ability to synthesise pyrethrins (1999)
    Springer
    Plant cell reports 19 (1999), S. 156-160 
    Details
    ISSN: 1432-203X
    Keywords: Key words Chrysanthemum cinerariaefolium ; Differentiation ; Mass propagation ; Pyrethrins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Rapid mass propagation of Chrysanthemum cinerariaefolium from young flower heads was developed to compare the ability of callus, in vitro shoots and rooted plants, and original plants to synthesize pyrethrins. The ability to synthesise all six pyrethrin components increased with differentiation. Jasmolin II and cinerin II were the main products present in mother plant shoots, whereas pyrethrin I was the greater component present in callus and in vitro plants. Clonal propagation increased the pyrethrin I content compared to that of plant shoots and young flowers. Total pyrethrin content was the same in in vitro and plant shoots, but lower in these shoots than in young flowers. The pyrethrin I/pyrethrin II ratio, which is directly related to insecticidal activity, varied from 3.4 in in vitro shoots to 0.87 in mother plant shoots and young flowers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050726
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Gas Exchange and Chlorophyll Fluorescence in Symbiotic and Non-Symbiotic Ryegrass Under Water Stress (1999)
    Springer
    Photosynthetica 37 (1999), S. 107-112 
    Details
    ISSN: 1573-9058
    Keywords: endophyte ; Lolium perenne ; Neotyphodium lolii ; photosynthesis ; stomatal conductance ; transpiration rate ; water potential
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The symbiotic association of endophyte fungus, Neotyphodium lolii, and ryegrass improves the ryegrass resistance to drought. This is shown by a 30 % increase in the number of suckers in infected plants (E+), compared to plants lacking endophyte (E−), and by a higher water potential in the E+ than E− plants. The E+ plants have higher stomatal conductance (g s), transpiration rate, net photosynthetic rate (P N), and photorespiratory electron transport rate than the E− plants. The maximal photochemical efficiency (Fv/Fm) and the actual photochemical efficiency (ΦPS2) are not affected by the endophyte fungus. The increase in P N of the E+ plants subjected to water stress was independent from internal CO2 concentration. An increased P N was observed in E+ plants also in optimal water supply. Hence the drought resistance of E+ plants results in increased g s, P N, and photorespiratory electron transport rate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007027131613
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Growth, Stomatal Conductance, Photosynthetic Rate, Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase and Phosphoenolpyruvate Carboxylase Activities during Rooting and Acclimatisation of Rosa Hybrida Plantlets (2000)
    Springer
    Photosynthetica 38 (2000), S. 629-634 
    Details
    ISSN: 1573-9058
    Keywords: mineral salts ; relative humidity ; rose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The rooting of shoots of micropropagated Rosa hybrida cv. Madame Delbard was conducted on MS medium with 30 kg m−3 sucrose or on hydroponic medium (containing less mineral salts), under higher photosynthetic photon flux density (PPFD) (100 in comparison with 45 µmol m−2 s−1) and flushed by ambient air [AC, 340 µmol(CO2) mol−1] or by CO2-enriched air (EC, 2 500 µmol mol−1) and lower relative humidity (80–90 % vs. 96–99 %). This cultivation led to plantlets with longer roots and adventitious root formation. Net photosynthetic rate and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) activities, RuBPCO/phosphoenolpyruvate carboxylase activities ratio, and starch accumulation increased under these conditions. After 14 d, plantlets had functional stomata and could be acclimated on open benches without gradual decrease in relative humidity. The percentage of survival was higher when the rooting took place in EC than in AC. However, the advantage acquired during rooting phase by plantlets cultured in liquid medium was not maintained after 4 weeks of acclimatisation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1012425927121
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    Paper (German National Licenses)
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