ISSN:
1573-0603
Keywords:
interleukin 1
;
interleukin 2
;
interleukin 3
;
interleukin 4
;
lymphokine bioassay
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Sensitive microassay methodologies are described for the bioassay of interleukins 1, 2, 3, and 4 in both serum-free and serum-containing culture supernatants. Interleukins 2, 3, and 4 are measured directly by their growth-promoting activities on the CT6, FDC-P1, and HT-2 indicator cell lines, respectively. Interleukin 1 is assayed indirectly by its ability to stimulate interleukin 2 production by LBRM-33 1A5 lymphoma cells in the presence of phytohemagglutinin. Quantitation is based on measurement of [3H]thymidine incorporation into cellular DNA. The bioassays are performed in microcultures and are semiautomated so that panel testing of small volumes of interleukin-containing culture supernatants can be readily accomplished within 3 d.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01404599
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