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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 10 (1986), S. 371-379 
    ISSN: 1432-0983
    Keywords: Yeast mtDNAs ; Hybridizable sequences ; Inversions ; Yeast taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sequences hybridzing to mitochondrial DNA probes from Saccharomyces cerevisiae have been mapped in six mitochondrial genomes from the Dekkera/Brettanomyces yeasts and in mtDNA from the closely related Eeniella nana. Sequence order for the 34.5 kbp mtDNA of E. nana is identical to that for mtDNAs from B. custersianus (28.5 kbp) and B. naardenensis (41.7 kbp) thereby suggesting that the former yeast is affiliated with the latter two species. A closer relationship is suggested for D. intermedia and D. bruxellensis as mtDNAs from these yeasts, 73.2 and 85.0 kbp respectively, have the same sequence order and mostly common restriction endonuclease sites. Differences between the two molecules are reminiscent of those found in mtDNA polymorphisms of S. cerevisiae strains thereby suggesting that the two Dekkera yeasts are variants of a single species. An unusual feature of the Dekkera species mtDNA is an inversion of the cytochrome b hybridizable region relative to the LrRNA sequence. Likewise mtDNA from B. anomalus (57.7 kbp) has an inversion of the cytochrome oxidase subunit 1 sequence with respect to the LrRNA sequence. By contrast the largest mtDNA (101.1 kbp) from B. custersd has the cytochrome b and LrRNA sequences in the same orientation. In addition hybridizable regions in this mtDNA are found in three clusters that are separated by several thousand base pairs of sequence deficient in restriction endonuclease sites. This observation together with the low guanine and cytosine content of the mtDNA suggests that the regions separating the sequence clusters are mostly adenine and thymine residues.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1432
    Keywords: Phylogenetic tree ; mtDNA evolution ; Cytochrome oxidase subunit 2 sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial genomes from yeasts in the Dekkera/Brettanomyces/Eeniella group vary in size from 28 to 101 kb. Mapping of genes has shown that the three smallest genomes, of 28–42 kb, have the same gene order, whereas the three larger mitochondrial DNAs of 57–101 kb are rearranged relative to the smaller molecules and between themselves. To examine the relationships between these genomes, a phylogenetic tree has been constructed by sequence comparison of the mitochondrialencoded cytochrome oxidase subunit gene (COX2) from the six species. Contrary to expectation, the tree shows that the larger rearranged genomes are more closely related than the smaller mtDNAs. This result indicates that the gene order of the smaller mtDNAs (28–42 kb) is ancestral and that larger mtDNA molecules (57–101 kb) are more prone to rearrangement than smaller forms.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-4927
    Keywords: RAPD PROFILING ; PARENTAGE ; PATERNITY ; IDENTIFICATION ; KOALA ; PHASCOLARCTOS CINEREUS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Highly repeatable randomly amplified polymorphicDNA (RAPD) markers were developed for parentage studiesin the koala (Phascolarctos cinereus). Of the 25 RAPDprimers screened, 5 (20.0%) produced 32 repeatable polymorphic RAPD bands (average/primer = 6.4± 4.2). A high level of polymorphism was observedfor each group of koalas (Featherdale, 71.9%; Lone Pine,84.4%). All 25 koalas could be uniquely identified using either RAPD or microsatellite markers. Of the32 RAPD markers generated in koalas, 25 were informativefor parentage analyses. These RAPD markers successfullydetermined both parents to three offspring and a male parent to a fourth offspring.Paternity analysis (where the female parent is known)succeeded in assigning the correct male parent to sevenoffspring. Our RAPD–PCR method generatesinformative genetic markers that are useful for parentagedetermination and individual identification of captivekoalas. This would provide genetic analysis to zoos andwildlife parks as a low-cost alternative to the more expensive microsatellite markers.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Biochemical genetics 36 (1998), S. 381-393 
    ISSN: 1573-4927
    Keywords: GENETIC DIVERSITY ; POPULATION STRUCTURE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Randomly amplified polymorphic DNA (RAPD)variation in populations of the koala, Phascolarctoscinereus, was investigated, revealing significantdifferences in the level of diversity between southernand northern regions of eastern Australia. Of the20 polymorphic RAPD markers identified in koalas, 4-7were polymorphic in southern populations, while 12-17were polymorphic in northern populations. Analysis of molecular variance revealed a significantdifference in the estimated variance between koalas fromnorthern and those from southern regions (P 〈 0.001),where populations from the north were greater than twice as variable as their southerncousins. The total genetic diversity observed wasattributed to regional differences (30.91%), populationdifferences within a region (11.77%), and differencesamong individuals within a population (57.32%). Forthe within-region analyses, a large proportion of thegenetic diversity was attributable to individualdifferences within a population, 80.34% for the north and 91.23% for the south. These resultsdemonstrate that RAPD markers are useful for determiningpopulation structure among koalas.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-4927
    Keywords: TELEOST ; BARRAMUNDI ; INSULIN-LIKE GROWTH FACTOR I
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Barramundi (Lates calcarifer) is ateleost of the superorder Acanthopterygii.Barramundi IGF-I cDNA was cloned and the distribution ofalternative transcripts in various barramundi tissueswas investigated using rt-PCR and RPA. It was demonstrated thatin barramundi tissues, IGF-I mRNAs were represented bytwo transcripts corresponding to the reported salmonidEa-2 and Ea-4. The acute effect of GH on hepatic IGF-I mRNA levels was investigated. Seven hoursafter intraperitonal administration of either 6 μg ofrecombinant bream GH/g body weight or saline, nosignificant increase in the levels of either of the two transcripts could be observed. The presenceof GH receptors in the barramundi liver was demonstratedin binding assays using recombinant bream GH and livermembrane preparations. An analysis of a barramundi IGF-I genomic sequence encompassing the threeexons that encode the E domain suggested that thepattern of splice site utilization is determined by thedegree of homology of the splicing signals to the consensus splice site sequences.
    Type of Medium: Electronic Resource
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