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Effect of the polygalacturonase inhibitor from pea on the hydrolysis of pea cell walls by the endopolygalacturonase from Ascochyta pisi (1985)

TURNER, J. G. ; HOFFMAN, R. M.

Oxford, UK : Blackwell Publishing Ltd

Plant pathology 34 (1985), S. 0

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ISSN: 1365-3059

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Exo–polygalacturonase (exo–PG) and endo–PG from Ascochyta pisi rapidly adsorbed to insoluble fragments of cell walls from pea leaflets as did the endo–PG inhibitor that was isolated from these organs. The wall fragments were hydrolysed by the exo– and endo–PG but the rates were, respectively, only 1–8 and 8% of the rate of hydrolysis of soluble polygalacturonic acid (PGA). Although the endo–PG inhibitor rapidly and completely inhibited the hydrolysis of soluble PGA by the endo–PG it had no effect on the rate of hydrolysis of pea cell wall fragments by this enzyme. Extracts of the intercellular fluid from pea leaflets contained only 4% of the inhibitor present in that organ and the remainder was presumably intracellular. These findings indicate that the endo–PG inhibitor may be less effective in vivo than was previously suspected.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1365-3059.1985.tb02760.x

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Isolation and partial characterization of a mutant of Penicillium funiculosum for the saccharification of straw (1985)

Hoffman, R. M. ; Wood, T. M.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 27 (1985), S. 81-85

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ISSN: 0006-3592

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Clearing of agar plates containing ball-milled, delignified straw has been used for screening mutants of Penicillium funiculosum IMI 87160 iii. The effects of glycerol and a number of sugars on the clearing were investigated for selecting derepressed mutants. The β-glucosidase synthesis by one such mutant, C22c, in shake flasks containing straw was not repressed by 5% glycerol, whereas activities on filter paper, CM-cellulose, and p-nitrophenyl-β-xylosidase were only partially derepressed; xylanase was extensively derepressed. The evidence for separate control of the enzymes involved in the solubilization of straw is discussed.

Additional Material: 2 Ill.