ISSN:
1573-6776
Keywords:
Aspergillus aculeatus
;
carbon catabolite repression
;
cellulase
;
promoter
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract The 5′-upstream non-coding region of an FI-carboxymethyl cellulase (CMCase) gene of Aspergillus aculeatus No. F-50 was obtained and sequenced up to −777 nucleotide in pCMG23. The 5′-upstream region of different lengths were constructed and fused to a reporter gene (Escherichia coli lacZ) in pAN923-42BD, and the resulted constructs were introduced into the A. nidulans. The β-galactosidase activities of the transformant with 5′-upstream fragment of larger than 319 bp were expressed by the induction, but that of 109 bp drastically decreased to the basal level. This suggests that the region between −109 and −319 of the 5′-upstream non-coding region is involved in the regulation of the FI-CMCase expression.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005534925774
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