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Ceramide Prevents Neuronal Programmed Cell Death Induced by Nerve Growth Factor Deprivation (1995)

Ito, Akira ; Horigome, Kazuhiko

Oxford, UK : Blackwell Science Ltd

Journal of neurochemistry 65 (1995), S. 0

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ISSN: 1471-4159

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Abstract: We examined the ability of ceramide and sphingomyelinase (SMase) to prevent neuronal programmed cell death (PCD). We found that a cell-permeable ceramide analogue prevented neuronal PCD when applied to established sympathetic neuron primary cultures at the time of nerve growth factor (NGF) deprivation. Other amphiphilic lipids such as oleic acid failed to prevent cell death. Exogenous SMase also showed the same effect, probably by raising the intracellular ceramide level by sphingomyelin (SM) breakdown. Phosphocholine, another hydrolytic product of SM by SMase, did not prevent cell death. Other phospholipases, such as phospholipase C and phospholipase A2, could not prevent cell death. Given the recent findings that the SM cycle is activated to increase the intracellular ceramide level on NGF binding to the low-affinity NGF receptor (LNGFR) and that NGF binding to LNGFR suppresses apoptosis in neural cell lines, our results suggest the possibility of the SM cycle as a signaling mechanism transducing the PCD-preventing activity of NGF.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1046/j.1471-4159.1995.65010463.x

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Functional Expression of Human Monoclonal Antibody Genes Directed Against Pseudomonal Exotoxin A in Mouse Myeloma Cells (1989)

Nakatani, Tomoyuki ; Nomura, Noriko ; Horigome, Kazuhiko ; [et al.]

[s.l.] : Nature Publishing Company

Nature biotechnology 7 (1989), S. 805-810

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ISSN: 1546-1696

Source: Nature Archives 1869 - 2009

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: [Auszug] We have cloned immunoglobulin (Ig) genes from an Epstein-Barr (EB) virus-transformed human B cell line, FK-001, which produced a monoclonal human IgM that specifically binds Pseudomonas aeruginosa exotoxin A (Ex-A). Cloned Ig genomic DNA was introduced into Ig non-producing mouse myeloma cells, ...