ISSN:
1573-6776
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary The copy number of plasmid harboringE. coli K 12 strains was examined in fed batch cultivations in semisynthetic and synthetic media. Under conditions of high cell density (45–50 g dry weight/I) the plasmid copy number reached a maximum level between 200 and 400 copies per cell. A decrease of phosphate concentration in the medium was obtained similar to the increase of copy number. A high segregational and structural stability of vectors used in this work was observed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01026797
Permalink