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Separation of proteins via semicontinuous pH parametric pumping (1977)

Chen, H. T. ; Hsieh, T. K. ; Lee, H. C. ; [et al.]

Hoboken, NJ : Wiley-Blackwell

AIChE Journal 23 (1977), S. 695-701

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Details

ISSN: 0001-1541

Keywords: Chemistry ; Chemical Engineering

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Notes: A semicontinuous pH parametric pump for separating proteins was experimentally investigated using the model system haemoglobin and albumin on a Sephadex ion exchanger. The pump considered had a center feed between an enriching column and a stripping column and was operated batchwise during upflow and continuously during downflow. Various factors affecting separations were examined. It was shown that parametric pumping is capable of separating proteins with high separation factors.

Additional Material: 8 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/aic.690230511

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