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  • 1
    Electronic Resource
    Electronic Resource
    Synthesis of an active hybrid growth factor (GF) in bacteria: transforming GF-α/vaccinia GF fusion protein
    Amsterdam : Elsevier
    Gene 60 (1987), S. 175-182 
    Details
    ISSN: 0378-1119
    Keywords: Recombinant DNA ; domains ; epidermal growth factor ; mitogenic polypeptide ; phage A ; plasmid vector ; synthetic gene
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(87)90225-3
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A quantitative study of the action ofCentruroides sculpturatus toxins III and IV on the Na currents of the node of Ranvier (1983)
    Springer
    Pflügers Archiv 397 (1983), S. 90-99 
    Details
    ISSN: 1432-2013
    Keywords: Myelinated nerve fibre ; Voltage clamp ; Scorpion toxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract 1. In nodes of Ranvier treated with toxin III or IV from the scorptionCentruroides sculpturatus Ewing a strong positive pulse is followed by a transient shift of the descending branch of theI Na(E) curve to more negative values of membrane potential (cf. Meves et al. 1982). This effect was studied in more detail, using toxin concentrations between 0.8 and 3.3 μg/ml. 2. The change of theI Na(E) curve was accompanied by a shift of the kinetic parameters of both activation (time to peak, time constant τm) and inactivation (time constant τh1). The τm curve was shifted by the same amount as the descending branch of theI Na(E) curve while the shift of τh1 was somewhat smaller. The curve relating Na permeability to membrane potential became less steep and its lower part was shifted to more negative values of membrane potential. 3. The change of theI Na(E) curve was also accompanied by a change in the turning-on kinetics of the Na current. The normal signoidal time course was replaced by first-order kinetics. A strong hyperpolarizing prepulse restored the sigmoidal time course without abolishing the shift of the descending branch of theI Na(E) curve. 4. The transient change of theI Na(E) curve was not accompanied by a marked change in the ion selectivity of the Na channels: the equilibrium potential decreased only by 4–8 mV. 5. Ca slightly reduced the shift of the descending branch of theI Na(E) curve. The long-lasting inward Na current which follows a strong positive pulse in nodes treated with toxin III or IV was reduced by 7.8 mM Ca to 41% of the value measured in normal *1.8 mM) Ca. Mg was slightly less effective than Ca. 6. From the change of the Na permeability curve the percentage of Na channels transiently modified by a strong positive pulse was estimated as about 50%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00582045
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    4S oop RNA is a leader sequence for the immunity-establishment transcription in coliphage λ (1976)
    [s.l.] : Nature Publishing Group
    Nature 262 (1976), S. 112-116 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] oop RNA, which is initiated at the po promoter and is 81 nucleotides long, can function as a leader sequence for the λ immunity establishment transcription, previously believed to originate at a special promoter pre located in the y region. Thus, oop RNA seems to have a ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/262112a0
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  • 4
    Electronic Resource
    Electronic Resource
    An O-linked carbohydrate neutralization epitope of HIV-1 gp 120 is expressed by HIV-1env gene recombinant vaccinia virus (1992)
    Springer
    Archives of virology 126 (1992), S. 11-20 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Previous studies have disagreed about the presence of O-linked carbohydrate epitopes on gp 120 of HIV, although antibodies against short-chain O-linked glycans neutralize HIV infection and block syncytium formation in vitro. To settle this question, we analysed the O-linked glycans of gp 120 by chemical methods using purified HIV-1 gp 120 from cells infected with recombinant vaccinia virus solely expressing gp 160 or gp 120. Alkaline borohydride degradation of recombinant gp 120 released monosaccharides and also slightly larger structures (di/trisaccharides) by a β-elimination, confirming the presence of simple O-linked oligosaccharides. The functional activity as neutralisation epitopes of the O-linked oligosaccharides expressed on recombinant gp 120 was preserved, since fusion between uninfected CD 4+ cells and cells infected with recombinant vaccinia was blocked by monoclonal antibodies to the O-linked oligosaccharides of gp 120. Although the mechanism for HIV induction of O-linked oligosaccharide neoantigens is unknown, these results indicate that the O-linked neutralization epitopes are inherent to the glycoprotein itself, and that the unusual appearance of simple O-linked oligosaccharides on gp 120 is independent of any interaction between the host cell and retroviral genes other thanenv.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01309680
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  • 5
    Electronic Resource
    Electronic Resource
    Identification of three N-linked glycans in the V4–V5 region of HIV-1 gp 120, dispensable for CD4-binding and fusion activity of gp 120 (1994)
    Springer
    Archives of virology 134 (1994), S. 335-344 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Site-directed mutagenesis was used to study the biological significance of three N-linked glycans (linked to Asn 406, Asn 448, and Asn 463), situated in the CD4-binding region of gp120. Mutagenesis was carried out in a phage M13 system, and the mutatedenv genes were inserted into recombinant vaccinia virus (r-vaccinia virus). To evaluate if the level of expression affected the biological phenotype of mutant gp 120, we expressed the envelope glycoproteins using either a weak (7.5 K) or a strong (11 K) promoter of vaccinia virus. The expression of mutatedenv proteins was analyzed after infecting CD4-expressing HeLa cells with the r-vaccinia virus, by monitoring the ability of the infected cells to generate CD4-dependent syncytia.Env gene products lacking all three glycans as well asenv gene products lacking different permutations of one or two glycans were analyzed. All mutated gp 120 species had the expected electrophoretical mobility as anticipated from elimination of one, two, and three N-linked glycans, respectively. Moreover, all mutantenv gene products demonstrated the same capacity to induce formation of syncytia, irrespective of using the weak or strong promoter for expression. These data indicate that the three N-linked glycans studied are dispensable for HIVenv gene products to function in CD4-binding and the subsequent fusion step.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01310571
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    Paper (German National Licenses)
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