ISSN:
1662-8985
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
N-isopropylacrylamide (NIPAM)-ethylene oxide (EO) copolymer was grafted on filterpaper surface to obtain paper-based adsorptive membrane. Protein adsorption on and release from themembrane was evaluated under various conditions using humanized monoclonal antibody as modelprotein. The results obtained indicated that amphiphilic copolymer modified filter paper could beused as a novel chromatographic membrane for protein separation/purification. Comb-blockcopolymers of NIPAM with N-vinylpyrrolidone (VP) were prepared through RAFT polymerizationand macromonomer techniques. Surface modification of substrates, such as plastics and glass, wascarried out by treatment of the substrates in aqueous solution of the amphiphilic comb-blockcopolymer (adsorptive coating, pre-adsorption). It was found that the adsorbed copolymer layer wasstable in aqueous environment even when shear stress was applied. Protein adhesion on the materialsurfaces before and after the modification was investigated using lysozyme as model protein. Theresults obtained showed that the adsorptive coating layer on the material surfaces significantlyreduced non-specific protein adhesion
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/41/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.47-50.1311.pdf
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