ISSN:
0025-116X
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Physics
Notes:
In three consecutive steps involving halogenation, amination, and condensation with a convenient acyl derivative (1 or 2), α-hydro-ω-hydroxypoly(oxyethylene) (3) was transformed into poly(oxyethylene)s 7 containing 0,6 to 1,3 mol of 3-[3,17β-dihydroxy-1,3,5(10)-estratrien-7α-yl]butyroyl end groups or 0,7 mol of 3-oxo-4-androsten-17β-ylcarboxyl end groups per mol of polymer. Applying the method of affinity partitioning, the former poly(oxyethylene) derivative 7 was used to purify successfully Δ5→4 3-oxosteriod isomerase contained in a crude extract from Pseudomonas Testosteroni.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/macp.1978.021790501
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