ISSN:
1432-2048
Keywords:
Gas exchange
;
Nicotiana
;
Photosynthesis (C3)
;
Ribulose-1,5-bisphosphate carboxylase/oxygenase kinetics
;
Transgenic tobacco
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Transgenic tobacco (Nicotiana tabacum L. cv. W38) with an antisense gene directed against the mRNA of the ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) small subunit was used to determine the kinetic properties of Rubisco in vivo. The leaves of these plants contained only 34% as much Rubisco as those of the wild type, but other photosynthetic components were not significantly affected. Consequently, the rate of CO2 assimilation by the antisense plants was limited by Rubisco activity over a wide range of CO2 partial pressures. Unlike in the wild-type leaves, where the rate of regeneration of ribulose bisphosphate limited CO2 assimilation at intercellular partial pressures above 400 ubar, photosynthesis in the leaves of the antisense plants responded hyperbolically to CO2, allowing the kinetic parameters of Rubisco in vivo to be inferred. We calculated a maximal catalytic turnover rate, kcat, of 3.5+0.2 mol CO2·(mol sites)−1·s−1 at 25° C in vivo. By comparison, we measured a value of 2.9 mol CO2·(mol sites)−1·−1 in vitro with leaf extracts. To estimate the Michaelis-Menten constants for CO2 and O2, the rate of CO2 assimilation was measured at 25° C at different intercellular partial pressures of CO2 and O2. These measurements were combined with carbon-isotope analysis (13C/12C) of CO2 in the air passing over the leaf to estimate the conductance for transfer of CO2 from the substomatal cavities to the sites of carboxylation (0.3 mol·m−2·s−1·bar−1) and thus the partial pressure of CO2 at the sites of carboxylation. The calculated Michaelis-Menten constants for CO2 and O2 were 259 ±57 μbar (8.6±1.9μM) and 179 mbar (226 μM), respectively, and the effective Michaelis-Menten constant for CO2 in 200 mbar O2 was 549 μbar (18.3 μM). From measurements of the photocompensation point (Γ* = 38.6 ubar) we estimated Rubisco's relative specificity for CO2, as opposed to O2 to be 97.5 in vivo. These values were dependent on the size of the estimated CO2-transfer conductance.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00206296
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