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  • 1
    Electronic Resource
    Electronic Resource
    The coding site of chloroplast ferredoxin (1977)
    Springer
    Planta 137 (1977), S. 97-105 
    Details
    ISSN: 1432-2048
    Keywords: Chloroplast ; Ferredoxin-coding-site ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ferredoxins were isolated and purified from leaves of different species of Nicotiana and Petunia and from spinach leaves. Their spectral properties, degree of homogeneity, and molecular weights were determined. The preparations were further analyzed by polyacrylamide gel electrophoresis of tryptic hydrolysates. This allowed us to distinguish between not only ferredoxins of Nicotiana, Petunia, and spinach, but even ferredoxins of various Nicotiana species. We used the differences in tryptic peptide compositions as phenotypic markers to study the mode of inheritance of chloroplast ferredoxin to see whether the coding site is in the chloroplast or in the nucleus. Analysis of the tryptic peptide composition of ferredoxin from different interspecific hybrids of Nicotiana showed that the characteristics of both parental ferredoxins were present. The results indicate that the primary structure of at least the male ferredoxin is coded for in the nucleus. In some of the hybrids the relative contribution of the male parent appeared to be low, suggesting that the female genome (presumably that part located in the plastome) exerted a dominating influence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387545
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Qualitative and quantitative immunofluorescence studies of chloroplast ferredoxin (1977)
    Springer
    Planta 137 (1977), S. 279-286 
    Details
    ISSN: 1432-2048
    Keywords: Chloroplast ; Ferredoxin ; Immunofluorescence ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Antibodies were raised in rabbits against 2Fe−2S ferredoxin from N. tabacum L. The antibodies showed partial cross-reactivity in the double diffusion test with ferredoxins from Spinacia oleracea L., Petunia inflata Fries., P. axillaris Lam., Phaseolus vulgaris L., Chlamydomonas remhardii Dang. A complete cross-reaction was observed with ferredoxins from five other Nicotiana species, thus with this test it was impossible to discriminate between these ferredoxins. Therefore the following test was performed. Heterologous ferredoxin (i.e., ferredoxin other than from N. tabacum) was coupled covalently to Sepharose beads. Rabbit anti-N. tabacum-serum was then pre-incubated with this ferredoxin which resulted in complete abolition of cross-reactivity with free heterologous ferredoxin. However, the serum retained antibody activity against specific antigenic determinants of N. tabacum ferredoxin. When this serum was tested against ferredoxin purified from the hybrid: N. tabacum (♀)xN. glutinosa (♂) it gave a positive reaction. The relative content of maternal N. tabacum ferredoxin in the hybrid was estimated by using a fluorescent derivative of this specific antibody and estimating the cross-reactivity compared with that of artificial mixtures of pure N. tabacum and N. glutinosa ferredoxins. The hybrid contained 50% of maternal ferredoxin. This technique was also applied to ferredoxins of other species of Nicotiana and to the ferredoxin from the hybrid N. clevelandii (♀)xN. glutinosa (♂). We conclude that it provides a good test system for the study of the expression of chloroplast ferredoxin in Nicotiana hybrids in general.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388163
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Expression of a wild-type GBSS gene introduced into an amylose-free potato mutant by Agrobacterium tumefaciens and the inheritance of the inserts at the microsporic level (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 369-375 
    Details
    ISSN: 1432-2242
    Keywords: Complementation ; Granule-bound starch synthase ; Amylose content ; Gene expression ; Inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Granule-bound starch synthase (GBSS) catalyses the synthesis of amylose in starch granules. Transformation of a diploid amylose-free (amf) potato mutant with the gene encoding GBSS leads to the restoration of amylose synthesis. Transformants were obtained which had wild-type levels of both GBSS activity and amylose content. It proved to be difficult to increase the amylose content above that of the wild-type potato by the introduction of additional copies of the wild-type GBSS gene. Staining of starch with iodine was suitable for investigating the degree of expression of the inserted GBSS gene in transgenic amf plants. Of the 19 investigated transformants, four had only red-staining starch in tubers indicating that no complementation of the amf mutation had occured. Fifteen complemented transformants had only blue-staining starch in tubers or tubers of different staining categories (blue, mixed and red), caused either by full or partial expression of the inserted gene. Complementation was also found in the microspores. The segregation of blue- and red-staining microspores was used to analyse the inheritance of the introduced GBSS genes. A comparison of the results from microspore staining and Southern hybridisation indicated that, in three tetraploid transgenics, the gene was probably inserted before (duplex), and in all others after, chromosome doubling (simplex). The partial complementation was not due to methylation of the HPAII/MSPI site in the promoter region. Partially complemented plants had low levels of mRNA as was found when the GBSS expression levels were inhibited by anti-sense technology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223647
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  • 4
    Electronic Resource
    Electronic Resource
    Reduction of HTLV-I-infected cells in blood by leukocyte filtration (1993)
    Springer
    Annals of hematology 67 (1993), S. 295-300 
    Details
    ISSN: 1432-0584
    Keywords: Blood transfusion ; Leukocyte filtration ; Human T-lymphotropic virus type I
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Leukocyte filtration was performed with HTLV-I-infected blood and with blood supplemented with cultured HTLV-I-transformed cells. Reduction of infectivity upon leukocyte filtration was determined by the polymerase chain reaction (PCR) using primers indicative for the HTLV-I-pol andtax genes. Two different commercially available filters were used: a columnshaped cellulose acetate and a flat-bed polyester filter. Both filters yielded reduction of at least 310logs for cultured HTLV-I-infected cells. When blood from HTLV-I-infected individuals was used for filtration, the number of infected cells was reduced by 1–310logs. Although filtered blood as yet cannot be regarded as safe, it is concluded that leukocyte filtration of HTLV-I-infected blood potentially contributes to reducing the spread of HTLV-I by blood transfusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01696350
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Biosynthesis of ferredoxin in Chlamydomonas reinhardii (1979)
    Springer
    Planta 145 (1979), S. 351-356 
    Details
    ISSN: 1432-2048
    Keywords: Chlamydomonas ; Ferredoxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The selective action of the antibiotics chloramphenicol and cycloheximide on the synthesis of ferredoxin in liquid cultures of Chlamydomonas reinhardii was studied. Highly specific antibodies raised against Chlamydomonas ferredoxin were used to determine the in vivo synthesis of apoferredoxin and conversion into native protein. The results indicate that 80S ribosomes are involved in the synthesis. Chlamydomonas cells growing in the absence of iron did not synthesize immunologically detectable amounts of ferredoxin. We suggest that this is based upon feed-back inhibition of apoferredoxin synthesis at the translational level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388360
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Asymmetric membrane filters for the removal of leukocytes from blood (1991)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 25 (1991), S. 1459-1480 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: As part of a study on the mechanisms of leukocyte filtration, the influence of pore size distribution on filter efficiency was investigated. Conventional leukocyte filters are not suitable for model studies, as these filters are composed of tightly packed synthetic fibers, with a poorly defined porous structure. Therefore, open cellular polyurethane membranes with pore size distributions varying from approximately 15 to 65 μm were prepared. Filtration experiments with stacked packages of these membranes showed that leukocytes are best removed (〉99%) by filters with a pore size distribution of 11-19 μm. These pore sizes approach the size of leukocytes (6-12 μm). However, due to fast clogging, blood flow through these filters is rapidly reduced, which results in a low filter capacity. With an asymmetric membrane filter, in which the pore size decreases from about 65 to 15 μm in the direction of blood flow, both moderate removal of leukocytes (〉80%) and maintenance of flow (∼0.2 mL/s) are obtained. This results in efficient leukocyte removal. From cell analysis of both filtrate and filter, it is concluded that adhesion rather than sieving is the major filtration mechanism. Thus, further optimization of the filter may be achieved by surface modification.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820251205
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