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  • 1
    Electronic Resource
    Electronic Resource
    Hydrolysis of riboflavin 5′-phosphate by phosphatases of rat kidney (1967)
    Springer
    Histochemistry and cell biology 8 (1967), S. 283-287 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 1. Hydrolysis of riboflavin 5′-phosphate was studied by biochemical and histochemical methods and the results were compared with those attained with sodium β-glycerophosphate. 2. Riboflavin 5′-phosphate was hydrolyzed both at the acid and the alkaline range. The pH optima were 5.3 and 9.2. 3. The K m values were 1.7×10−3 M at pH 9.2 and 1.0×10−3 M at pH 5.3. 4. The activities that hydrolyzed riboflavin 5′-phosphate and sodium β-glycerophosphate could not be separated from one another by Sephadex G-200 chromatography. 5. Riboflavin 5′-phosphate gave distinct histochemical reactions both at the alkaline and the acid pH and the localizations were like those attained with sodium β-glycerophosphate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306091
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Do molecular markers reflect patterns of differentiation in adaptive traits of conifers? (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 215-221 
    Details
    ISSN: 1432-2242
    Keywords: Key words Scots pine ; Molecular markers ; Isozymes ; Population structure ; Adaptive genetic variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have examined patterns of variation of several kinds of molecular markers (isozymes, RFLPs of ribosomal DNA and anonymous low-copy number DNA, RAPDs and microsatellites) and an adaptive trait [date of bud set in Scots pine (Pinus sylvestris L.)]. The study included Finnish Scots pine populations (from latitude 60°N to 70°N) which experience a steep climatic gradient. Common garden experiments show that these populations are adapted to the location of their origin and genetically differentiated in adaptive quantitative traits, e.g. the date of bud set in first-year seedlings. In the northernmost population, bud set took place about 21 days earlier than in the southernmost population. Of the total variation in bud set, 36.4% was found among the populations. All molecular markers showed high levels of within-population variation, while differentiation among populations was low. Among all the studied markers, microsatellites were the most variable (H–=0.77). Differences between populations were small, GST was less than 0.02. Our study suggests that molecular markers may be poor predictors of the population differentiation of quantitative traits in Scots pine, as exemplified here by bud-set date.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050268
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Do molecular markers reflect patterns of differentiation in adaptive traits of conifers? (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 215-221 
    Details
    ISSN: 1432-2242
    Keywords: Scots pine ; Molecular markers ; Isozymes ; Population structure ; Adaptive genetic variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have examined patterns of variation of several kinds of molecular markers (isozymes, RFLPs of ribosomal DNA and anonymous low-copy number DNA, RAPDs and microsatellites) and an adaptive trait [date of bud set in Scots pine (Pinus sylvestris L.)]. The study included Finnish Scots pine populations (from latitude 60°N to 70°N) which experience a steep climatic gradient. Common garden experiments show that these populations are adapted to the location of their origin and genetically differentiated in adaptive quantitative traits, e.g. the date of bud set in first-year seedlings. In the northernmost population, bud set took place about 21 days earlier than in the southernmost population. Of the total variation in bud set, 36.4% was found among the populations. All molecular markers showed high levels of within-population variation, while differentiation among populations was low. Among all the studied markers, microsatellites were the most variable (He=0.77). Differences between populations were small, GST was less than 0.02. Our study suggests that molecular markers may be poor predictors of the population differentiation of quantitative traits in Scots pine, as exemplified here by bud-set date.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225748
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Developing SSCP markers in two Pinus species (1999)
    Springer
    Molecular breeding 5 (1999), S. 21-31 
    Details
    ISSN: 1572-9788
    Keywords: SSCP ; Pinus pinaster ; Pinus sylvestris ; linkage map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study demonstrates the feasibility of generating sequence- based markers in Pinus species, from data available in electronic databases. Nucleotide sequences from 23 partially or fully characterized cDNAs or genomic sequences of pines were used to design PCR primers for amplifying targeted fragments of genomic DNA from Maritime and Scots pine. Various template DNA and MgCl2 concentrations, annealing temperatures, and buffer compositions were used to optimize the PCR amplifications. The polymorphism of 16 sequences was then investigated in a tree-generation inbred pedigree of Maritime pine and in a two-generation pedigree of Scots pine, using single-stranded DNA conformation polymorphism (SSCP) on polymerase chain reaction (PCR) products. The level of polymorphism was shown to be independent of (1) fragment size, (2) the presence or absence of introns in the amplified product and (3) temperature during electrophoresis. Mendelian segregation was tested for 5 SSCP markers in each species. Chromosomal locations of five genes were identified by linkage analysis with previously mapped markers in a genetic map of Maritime pine. The use of SSCP is recommended for constructing a transcriptional map for comparative mapping studies among pines and to provide useful ‘candidate genes’ for characterizing quantitative trait loci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009635226412
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    Paper (German National Licenses)
    Fulltext
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