ISSN:
1573-5044
Keywords:
Cell cluster
;
Daucus carota
;
morphological abnormality
;
somatic embryo
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Carrot somatic embryogenesis was serially observed using a cell cluster immobilizing system with Phytagel. Embryogenic cell clusters ranging in size from 32 to 63 μm were collected by filtration and used for somatic embryo induction. Of the 432 cell clusters, 253 grew, i.e., the size of these cell clusters increased, and 192 developed into globular embryos. Through serial observation, the number of somatic embryos produced from each cell cluster was identified. Cell clusters which developed into two or more embryos grew and developed slowly as compared with cell clusters which developed into single embryos. Serial observation also revealed that some cell clusters consisted of several parts, each of which independently grew as separate units. In cases where two growing parts fused into one embryo, morphological abnormalities such as curvature or lumps in their bodies were occasionally observed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006484023765
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