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  • 1
    Electronic Resource
    Electronic Resource
    Differential Morphogenetic Responses of Cotyledonary Explants of Vigna mungo (2000)
    Springer
    Biologia plantarum 43 (2000), S. 157-160 
    Details
    ISSN: 1573-8264
    Keywords: apical dominance ; in vitro flowering ; regeneration ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The morphogenetic responses of cotyledonary nodal explants of Vigna mungo (L.) Hepper cv. VBN1 cultured on the same Murashige and Skoog's medium, B5 vitamins, and 13.31 µM N6-benzylaminopurine showed variations in the pattern of multiple shooting and morphology of leaves in dependence on initial explants (presence/absence of cotyledons). The regenerated shoots elongated in the initial medium and most of them rooted in the presence of 2.41 µM indole-3-butyric acid, and flowered in vitro. Rooted plants could be transferred to the field after hardening.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026587904785
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The role of chemical cues in host finding and acceptance by Callosobruchus chinensis (2000)
    Springer
    Entomologia experimentalis et applicata 96 (2000), S. 213-219 
    Details
    ISSN: 1570-7458
    Keywords: herbivore ; host finding ; olfactometer ; volatiles ; oviposition ; discrimination ; Coleoptera ; Bruchidae ; pest control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We studied the response of female Callosobruchus chinensis to chemical cues emitted by cowpea seeds at different stages of bruchid infestation (uninfested, egg carrying, L1-, and L4-infested). Olfactory attractiveness was determined in Y-tube olfactometer assays by testing individual seed categories against either clean air or uninfested seeds. Oviposition preferences between uninfested and infested seeds were determined in petri-dish choice-experiments. The olfactometer assays revealed that weevils discriminate between seeds containing different stages of developing bruchids on the basis of olfactory cues. While odors from uninfested and egg-carrying seeds acted as attractants, odors from L1- and L4-infested seeds failed to induce a positive response by the bruchids. When given a choice between uninfested and infested seeds in the olfactometer, weevils preferred uninfested seeds over L1- and L4-infested seeds, but failed to distinguish between uninfested and egg-carrying seeds. In the oviposition experiment as well, bruchids showed a distinct preference for uninfested seeds when offered in combination with L1- and L4-infested seeds. This experiment further showed a reduced acceptance of egg carrying seeds. Our results indicate that C. chinensis females use chemical information during both host searching and host acceptance. Volatiles from uninfested or egg carrying seeds act as attractants, while deterrence increases as development of bruchid immature stages progresses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004016127866
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Factors affecting regeneration of pigeonpea (Cajanus cajan L. Millsp) from mature embryonal axes (2000)
    Springer
    Plant growth regulation 30 (2000), S. 31-36 
    Details
    ISSN: 1573-5087
    Keywords: embryonal axes ; light ; pigeonpea ; regeneration ; shoot organogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Regeneration of viable plants was obtained via organogenesis from mature embryonal axes explants of pigeonpea. Shoots were produced from the apical region of embryonal axes after 20 days of dark incubation on modified Murashige and Skoog (1962) medium containing 8.86 μM benzylaminopurine (BAP) and 1.07 μM α-naphthalene acetic acid (NAA). When the explants were cultured under light-dark (16--8 hrs) conditions, shoots were initiated only after 65 days of culture initiation. The explants lost their ability to regenerate plantlets when they were cultured in continuous light. Regenerated shoots elongated either in the same medium or in MS basal medium. About 40% of the elongated shoots sequentially produced simple, bifoliate and finally trifoliate leaves instead of producing trifoliate leaves directly. The elongated shoots were rooted efficiently upon transferring them to half strength MS medium supplemented with 2.41 μM indole-3-butyric acid (IBA). Rooted plantlets were successfully transferred to soil after hardening.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006394402210
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  • 4
    Electronic Resource
    Electronic Resource
    Factors affecting in vitro flowering and fruiting of green pea (Pisum sativum L.) (2000)
    Springer
    Euphytica 115 (2000), S. 65-74 
    Details
    ISSN: 1573-5060
    Keywords: cotyledonary node ; culture medium ; in vitro flowering ; in vitro seeds ; regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Multiple shoots were efficiently regenerated from cotyledonary node and shoot tip explants of Pisum sativum within 15 days on MS medium containing B5 vitamins and supplelmented with 2.0 mgl-1 6-benzylaminopurine. The elongated shoots produced on the same medium were excised and transferred to MS medium containing half strength ammonium nitrate (8.25 gml-1) and supplemented with auxins (indole-3-butyric acid or naphthalene acetic acid) either alone or in combinations with gibberellic acid. Rooting and flowering were observed on the 7th and 15th day after their transfer to rooting medium. The flowers self-fertilised in vitro and produced mature pods within 25 days of rooting. These seeds were germinable both in vitro and in vivo. In vitro seeds sown in pots under field conditions developed into flowering plants, and subsequently produced pods with viable seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003982900117
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  • 5
    Electronic Resource
    Electronic Resource
    Plant regeneration through somatic embryogenesis from mature leaf explants of Eryngium foetidum, a condiment (1999)
    Springer
    Plant cell, tissue and organ culture 56 (1999), S. 131-137 
    Details
    ISSN: 1573-5044
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Eryngium foetidum L. is an important plant cultivated as a leafy vegetable and for its essential oil, which are of high economic value in international trade market. Plants were regenerated through somatic embryogenesis from mature leaf explants of field grown plants. Leaf explants produced dark brown, compact callus on Linsmaier and Skoog (LS) medium with the combination of 1.0 mg l-1 2,4-dichlorophenoxy acetic acid (2,4-D) and 1.0 mg l-1 benzylaminopurine (BAP). Somatic embryos were induced from embryo-forming callus cultures on Murashige and Skoog (MS) medium supplemented with 0.1 mg l-1 2,4-D, 2.0 mg l-1 BAP and 1.0 mg l-1 gibberellic acid (GA3). Subsequently, conversion of these somatic embryos into plantlets occurred on MS medium supplemented with 1.0 mg l-1 GA3 and/or 0.1 mg l-1 BAP. The regenerated shoots were rooted and elongated on MS medium supplemented with 0.1 mg l-1 IAA and 1.0 mg l-1 GA3. These plantlets were hardened and transferred to the soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006228310864
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  • 6
    Electronic Resource
    Electronic Resource
    Regeneration of plantlets from cotyledon and embryonal axis explants of Vigna mungo L. Hepper (1998)
    Springer
    Plant cell, tissue and organ culture 55 (1998), S. 75-78 
    Details
    ISSN: 1573-5044
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sexually mature black gram plantlets were efficiently regenerated from seed-derived cotyledon and embryonal axis explants.The explants were capable of producing multiple shoots when cultured on modified MS medium containing B5 vitamins supplemented with 6-benzylaminopurine (BAP) and α-naphthaleneacetic acid (NAA) within 15 days. The regenerated shoots were elongated on MS basal medium and rooted on MS medium containing indole-3-butyric acid (IBA). Rooted plantlets were transferred to soil with 75% success, where they attained sexual maturity and produced viable seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026481310822
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    Paper (German National Licenses)
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