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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    The @journal of organic chemistry 49 (1984), S. 3660-3662 
    ISSN: 1520-6904
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of the American Chemical Society 117 (1995), S. 5389-5390 
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature biotechnology 19 (2001), S. 751-755 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] We have developed a protein-synthesizing system reconstituted from recombinant tagged protein factors purified to homogeneity. The system was able to produce protein at a rate of about 160 μg/ml/h in a batch mode without the need for any supplementary apparatus. The protein products were easily ...
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Archives of orthopaedic and trauma surgery 104 (1985), S. 7-14 
    ISSN: 1434-3916
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In order to clarify the long-term results of Perthes' disease and to elucidate the residual deformities, including those of the acetabulum, related to coxarthrosis, 51 patients who had had Perthes' disease were studied clinically and radiologically. Altogether there were 56 affected hips and radiographs taken from onset of disease to maturity were available in all cases. They were followed up for an average of 18 years. Thirteen hips (23%) revealed coxarthrosis. Head deformity, steepness of the lateral edge of the acetabulum, and insufficient acetabular coverage of the femoral head were noted in them both in adulthood and in the active phase of disease. Among these deformities, steepness of the lateral edge of the acetabulum was found to be the most significant prognostic factor and led to coxarthrosis of the affected hip even in the younger adult. Acetabular roof angle, which is a newly designed radiographic measurement, is a good indicator of the prognosis in Perthes' disease.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Archives of orthopaedic and trauma surgery 120 (2000), S. 455-457 
    ISSN: 1434-3916
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The origin and natural course of solitary bone cysts (SBC) still remain controversial. Knowledge of the process of cyst formation and enlargement would be helpful for investigating its pathogenesis. Only two papers have described a radiodense nidus of the diaphysis as a precursor. Their cases were unique in that the initial lesions were in the diaphysis and that the cysts did not abut onto the epiphyseal line. This study reports a case in a patient with a tiny erosive lesion in the endosteal surface of the humeral metaphysis, which developed expansively into a typical large cyst over 6 years. Serial roentgenograms taken every year demonstrated the process of cyst enlargement. This is the first longitudinal study of a case with SBC documented from its onset.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-0932
    Keywords: Key words Chemonucleolysis ; Chymopapain ; Hypertonic saline ; Intradiscal injection ; Intradiscal pressure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Although chemonucleolysis with chymopapain is a long-established treatment for lumbar intervertebral disc herniation, serious complications have been reported. Accordingly, alternative substances for chemonucleolysis have been sought. The main beneficial effect of chemonucleolysis derives from the decrease in intradiscal pressure. Several previous studies have investigated the relationship between physiological saline injection and disc mechanics in cadaveric specimens [2, 5, 16]. However, no previous study has assessed the intradiscal pressure after intradiscal injection of “hypertonic saline” in living animals. The present study compared the changes in intradiscal pressure after intradiscal injection of hypertonic saline with those after chymopapain injection. The lumbar intervertebral discs of 26 living rabbits were examined: 10% hypertonic saline was injected in ten rabbits, and chymopapain (10 pikokatal units) was injected intradiscally in another ten, with the remaining six being used as controls. The intradiscal pressure was measured at 1, 4, and 12 weeks after injection. The intradiscal pressure of the hypertonic saline-injected group at 4 weeks was significantly lower than that of the control group, but by 12 weeks it had recovered. On the other hand, that of the chymopapain-injected group remained significantly lower than that of the control group at 12 weeks. The results of this study found that hypertonic saline injected into the intervertebral discs temporarily decreased the intradiscal pressure.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Molecular and cellular biochemistry 5 (1974), S. 127-143 
    ISSN: 1573-4919
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary This review summarizes recent studies on the structure and function of myosin, and attempts to survey them critically. Merits and demerits of our original reaction mechanism of myosin-ATPase and its simplified variant proposed byTaylor et al. are described. Recent studies on the substructure of the myosin molecule and the reaction mechanism of the myosin-ATP system strongly suggest that the myosin molecule has two nonidentical heads. Various lines of evidence are also presented for the mechanism that ATP is hydrolyzedvia two different routes: one is the simple hydrolysis through the myosin-ATP complex, and the other is the one through the reactive myosin-phosphate-ADP complex. Our recent studies clearly demonstrate that F-actin accelerates markedly de-composition of the reactive myosin-phosphate-ADP complex. The molecular mechanism of this acceleration is analyzed by use of transient enzyme kinetic methods. Finally, the reaction mechanism of the myosinactin-ATP system is discussed in relation with the molecular mechanisms of the three fundamental steps in muscle contraction, i.e., the binding of the projections from myosin filaments with actin filaments, sliding of actin filaments past myosin filaments with movement of the projections, and detachment of the projections from actin filaments.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    International orthopaedics 4 (1980), S. 225-229 
    ISSN: 1432-5195
    Keywords: Spastic equinovarus ; Tendon transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Le transfert antérieur des longs fléchisseurs des orteils a été utilisé pour corriger la déformation en varus équin du pied spastique, tant chez l'adulte que chez l'enfant. Chez l'adulte, il s'agissait d'hémiplégies secondaires à un accident vasculaire cérébral, d'hémiplégies spastiques dues à une paralysie cérébrale, et de paraplégies médullaires spastiques. La plupart des enfants étaient atteints de paralysie cérébrale. Le transfert a paru indiqué en présence d'un pied varus équin avec fléchisseurs des orteils actifs, entraînant soit une griffe des orteils au cours de la phase pendulaire de la marche, soit une attitude en marteau irréductible. Cinquante-six malades ont été suivis plus de quatre ans. Dans tous les cas la correction du varus équin a été obtenue et s'est maintenue. Grâce à une correction satisfaisante, la stabilité de la cheville a été améliorée, les anomalies posturales au cours de la marche ont diminué et l'appareillage est devenu inutile. Ce travail démontre la supériorité du transfert des longs fléchisseurs des orteils chez de tels malades. La longueur des tendons utilisables permet de les transposer aisément sur la face dorsale du métatarse. La suppression de l'activité de muscles spastiques entraîne une amélioration de la marche et préserve la fonction des muscles jambiers antérieur et postérieur.
    Notes: Summary Anterior transfer of the long toe flexors was carried out for the treatment of spastic equinovarus foot deformity in both adults and children. Adults included those with hemiplegia subsequent to a stroke, spastic hemiplegia due to cerebral palsy and spastic spinal paraplegia. Most of the children had cerebral palsy. The transfer was indicated for an equinovarus foot with persistent activity of the toe flexors, which produced curling of the toes in the swing phase of the gait or a fixed hammer toe deformity. Fifty six patients were followed up for more than four years. In all cases correction of the equinovarus deformity was achieved and maintained. With satisfactory correction stability of the ankle improved, postural abnormalities during gait decreased and bracing was not required. This study demonstrates the advantage of the long toe flexors for muscle transfer in these patients. The length of tendon available permitted easy transfer to the metatarsal. The defunctioning of the spastic muscles allowed gait improvement and function of the tibialis posterior and tibialis anterior was preserved.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-0878
    Keywords: Key words: Smooth muscle ; Gizzard ; Differentiation ; Fibroblast cell ; Chicken (White Leghorn)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. We examined the in vitro differentiation of smooth muscle cells from undifferentiated cells of embryonic chicken gizzard. We used the gizzard from 7-day-old embryos (Hamburger and Hamilton’s stage 26–28) as a source of culture, since the expression of myosin is extremely low, and the gizzard consists of round cells, which are not stained by anti-smooth muscle myosin antiserum. When the dissections of the gizzard were cultured, they attached to the dish, and fibroblast-like cells migrated within 4 days after the culture. Then round cells migrated from the transplants over the layer of fibroblast-like cells. At 12–14 days after the culture, smooth muscle cells, which were ribbon-shaped and stained by anti-smooth muscle myosin antiserum, appeared in the layer of round cells. The dissected transplant itself was not stained by anti-smooth muscle myosin antiserum even after being cultured for 15 days. We concluded then that the smooth muscle cells were differentiated from the round cells, which spread on the layer of the fibroblast-like cells. We also observed the differentiation of smooth muscle cells when the cells separated from 7-day-old embryo cultured on the layer of cloned fibroblast cells. We suggest that the fibroblast-like cell may play an important role in the differentiation of smooth muscle cells.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Archives of orthopaedic and trauma surgery 95 (1979), S. 159-165 
    ISSN: 1434-3916
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Seit 1965 wurde die anterolaterale Verpflanzung der Psoas-Sehne als eine routinemäßige chirurgische Behandlung bei angeborener Hüftluxation angewendet. Die Sehnenverpflanzung wurde zusammen mit einer offenen Einrenkung durchgeführt, wenn intrakapsuläre Repositionshindernisse auffallend sind. Pfannenverbessernde Verfahren oder Beseitigung der Fehlstellung des coxalen Femurendes wurden dabei nicht durchgeführt. An unserer Klinik wurden 87 Hüftgelenke mit dieser Methode in der Zeit von 1965 bis 1973 behandelt. Die Patienten (38 Hüfte) konnten nachuntersucht werden. Die durchschnittliche Beobachtungszeit beträgt 8 Jahre. Als Kontrolle wurden 13 Hüften, die offen eingerenkt waren, untersucht. Reposition des Femurkopfes und Entwicklung der Pfanne und des coxalen Femurendes wurden röntgenologisch nach dem Schema von Severin beurteilt. Es lag keine Reluxation vor. Das Ergebnis der offenen Einrenkung und der Psoas-Verpflanzung ist in 50% als I. oder II. Grad zu bezeichnen. Diese Erfolgsquote ist höher als die der Kontrolle, bei der 23% als I. oder II. Grad beurteilt wurden. Eine bessere Konzentrizität des Femurkopfes kann durch CE-Winkel (Wiberg) nachgewiesen werden. Es wurde festgestellt, daß die Pfannenentwicklung besser beeinflußt werden kann, wenn die Psoas-Verpflanzung bei Kindern unter 1 Jahr durchgeführt wurde. Die Pfannenentwicklung kann bei ä1teren Kindern wenig beschleunigt werden. Der detorquierende EMU dieser Psoas-Verpflanzung scheint fraglich zu sein, weil die Detorquierung der operierten Gruppe die der Kontrollgruppe nicht übersteigt.
    Notes: Summary Since 1965, anterolateral transfer of the psoas muscle has been employed by us as a routine surgical treatment for congenital dislocation of the hip joint. The transfer was carried out in association with open reduction in those patients in whom an intracapsular obstruction was evident, but corrective measures for acetabular dysplasia or cervicocapital deformity of the femur were not performed in this series. We treated 87 hip joints with this procedure from 1965 to 1973. In 38 hip joints, both open reduction and the psoas transfer were followed up for an average of 8 years. Thirteen hip joints with open reduction alone were included as controls. Position of the femoral head and development of the acetabulum and proximal end of the femur were assessed on X-rays, mainly after Severin's criteria. Redislocation was not encountered. Fifty percent of the hip joints with open reduction and the psoas transfer were classed as grade I or II. This figure is higher than that of controls, to which 23% were classed as grade I or II. Better concentricity of the head was achieved as determined by the CE angle (Wiberg). Development of acetabulum occurred when the transfer had been carried out in children under 1 year of age and was less likely in the older children. The detorsional effect of the psoas transfer remained questionable since detorsion in the transferred group did not exceed that of the controls.
    Type of Medium: Electronic Resource
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