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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of the American Chemical Society 110 (1988), S. 8733-8734 
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 37 (1989), S. 1026-1031 
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 39 (1991), S. 770-777 
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 153 (1997), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: An archaeal phosphoenolpyruvate carboxylase (PEPC) was purified from an acidophilic extreme thermophile, Sulfolobus acidocaldarius. The native enzyme was a homotetramer of 260±20 kDa molecular mass composed of 60±5 kDa subunits. The enzyme appeared to have a temperature optimum of 90°C and a pH optimum of 8.0. The activity of S. acidocaldarius phosphoenolpyruvate carboxylase was inhibited by l-aspartate and l-malate, but not enhanced by any metabolites. In comparison to the enzymatic and molecular properties of all other phosphoenolpyruvate carboxylases including another archaeal entity from the hyperthermophilic methanogen Methanothermus sociabilis, the archaeal phosphoenolpyruvate carboxylases were quite different from bacterial and eucaryal counterparts, and their small size and the lack of positively allosteric regulation were likely to be peculiar to the enzyme of the domain Archaea.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 73 (1990), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The distribution of heterotrophic bacteria in polluted coastal and unpolluted pelagic seawaters was studied using a 14C-MPN method with either five of seven kinds of 14C-organic compounds as substrates. The total number of heterotrophic bacteria in pelagic waters ranged from 9.2 × 103 to 5.4. ? 104 cell/ml and more than 85% of the heterotrophic bacteria were represented by obligate oligotrophs. In coastal waters, the number of heterotrophs was one order of magnitude higher (av. 3.5 ? 105 cells/ml), and eutrophic and facultatively oligotrophic bacteria were predominant. Oligotrophs in pelagic waters had a high specificity for the utilization of amino acids, especially glycine, and acetate-utilizing bacteria were scarce. The in situ maximum uptake rates of glutamate and glycine were much higher than those of glycolate and acetate. Acetate uptake rates were extremely low or not detectable in pelagic waters. The specificity of uptake kinetics is assumed to depend on the existence of obligate oligotrophs as dominant bacteria in pelagic seawater.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Microbial ecology 7 (1981), S. 123-130 
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Eighteen strains of obligately oligotrophic bacteria that grow in a medium containing 1 mg of organic carbon per liter and do not grow in a rich medium (5 g/liter of nutrient) were isolated as dominant organisms from the oligotrophic water of Lake Biwa. The growth properties of these, especially of five strains, were examined. The maximum cell yield ranged from 8.5×104/ml to 2.3×106/ml, and their doubling times ranged from 6.6/h to 11.8/h in LT10−4 medium (0.5 mg trypticase and 0.05 mg yeast extract in 1 liter of filtered and aged lake water). They also showed good growth in lake water medium without adding nutrients. The optimum concentrations for their growth were 5 mg/1, 5–50 mg/1, 50 mg/1, or 500 mg/1, depending on the strains. They utilized glutamate, glycine, serine, and glycolate, but not acetate, proline, or leucine. Several properties were examined. Their growth properties were very different from those of oligotrophs or oligocarbophiles isolated by other researchers.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In oligotrophic waters, not only community structure but also physiological properties of heterotrophic bacteria are influenced by the concentration of organic matter. The relationship between growth rate of two facultatively oligotrophic strains ofAeromonas sp. No. 6 andFlavobacterium sp. M1 was studied in comparison with that of two eutrophic strains ofEscherichia coli 7020 andFlavobacterium sp. M2. These strains had two or three different substrate constants (Ks values) depending on substrate concentrations: Ks values for the two former were remarkably lower than those for the two latter. For instance, Ks value forAeromonas sp. No. 6 was about 8.9μM when substrate concentration was greater than 53μM and about 1.1μM when substrate concentration was less man 53μM. InE. coli the Ks value was about 260μM at greater than 5600μM and about 47μM at less than 5600μM substrate concentration. Uptake kinetics ofAeromonas sp. grown in a medium containing 2.7 mM glutamate (H-cell) and 0.11μM glutamate (L-cell) have been determined for the intact cells. H-cell had two distinct values of Km for glutamate assimilation and respiration, and L-cell had three distinct values of Km for glutamate assimilation and respiration: In H-cell Km of assimilation was 2.8×10−7 M and 1.5×10−4 M, and Km of respiration was 2.3×10−7 M and 1.7×10−4 M; in L-cell Km of assimilation was 7.4×10−8 M, 8.3×10−6 M, and 1.3×10−4 M, and Km of respiration was 2.5×10−7, 8.9×10−6M, and 1.7×10−4 M. More than 60% of glutamate taken up by the H- and L-cells was respired when the substrate concentration was less than 10−6 M, although at greater than 10−6 M, 50% and 30% of glutamate was respired by H-cells and L-cells, respectively. These results suggest that the facultatively oligotrophic bacteria grow with high efficiency in environments with extremely low nutrient concentration, such as oligotrophic waters of lakes and ocean, as compared with in their growth in conditions of high nutrient concentraton, such as nutrient broth.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 358 (1997), S. 185-191 
    ISSN: 1573-5117
    Keywords: Microalgae ; bacterial role ; stimulativeeffects ; inhibitory effects ; interaction ; bio-control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several examples of stimulative and inhibitoryeffects of bacteria on microalgal growth areintroduced, and the importance of bacteria in algalmass culture is investigated. Diatoms are often usedas live food for planktonic larvae of sea urchin andbivalves. Monodispersed Chaetoceros ceratosporum hasbeen cultivated by using clean, high nutrient content,deep seawater (DSW). However, the growth rate and cellyield of diatoms fluctuated, to relatively largeextent, with the season that DSW was collected. Whensome bacterial strains isolated from DSW were added tothe culture, diatom growth was often stimulated and arelatively constant cell yield was obtained. Anotherdiatom species, C. gracilis, was also stimulated byadding some bacterial strains to cultures. Thepositive effect of bacteria on diatoms was observednot only for planktonic species, but also on attachedspecies. A benthic diatom, Nitzschia sp., wasstimulated by a bacterial film of Alcaligenes on thesurface of the substratum. On the other hand, a strainof Flavobacterium sp. isolated from natural seawaterduring the decline period of an algal bloom had a strongalgicidal effect on the red tide plankton,Gymnodinium mikimotoi. Recent reports demonstratethat many bacterial strains have significantalgicidal effects on many species of red tideplankton. These results indicate that bacterialeffects should be taken into account to obtain stablemass culture of food microalgae.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1573-5117
    Keywords: high altitude lake ; Himalayan lake ; trophic status ; glacier silt ; Nepal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The trophic status and water quality of Lake Tilitso (4920 m above sea level) in a high altitude region in central Nepal were surveyed in September, 1984. The lake is rather large with a maximum depth of 95 m and a surface area of 10.2 km2. The lake water was turbid due to glacier silt and the euphotic layer was only 5 m deep. The nutrient concentration was very low with total phosphorus concentration 1–6 µg l−1, and DTN concentration 0.10–0.22 mg l−1. The phytoplankton biomass and chlorophyll-a concentration were also low. Primary production was estimated to be about 12 mg C m−2 d−1. The concentrations of particulate matter and most cations and bacterial number were higher in the epilimnion than in the hypolimnion. The trophic status of this lake was estimated as ultraoligotrophic.
    Type of Medium: Electronic Resource
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