ISSN:
1435-232X
Keywords:
Key words Pentamer repeats
;
H19
;
PEN11B
;
Imprinting
;
Placenta
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Using a repetitive sequence of tandemly arrayed pentanucleotides in the human H19 3′-flanking region, we isolated a phage clone (λ PEN11) which localized to chromosome 11p15.5. The λ PEN11 phage encodes a 2.3-kb cDNA consisting of seven exons at least. The gene was mainly expressed in brain and pancreas (and less abundantly in testis), and demonstrated differential allele usage, with maternal expression being predominant in placenta, which indicates the gene is an atypical imprinted gene. While the pentamer repeat might contribute to this effect, it is also possible that the differential allele usage might reflect the local chromosomal structure known as the imprinting domain.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s100380050096
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