ISSN:
1432-203X
Keywords:
Key words Shoot tips
;
Cryopreservation
;
Vitrification
;
Taro [Colocasia esculenta (L.) Schott.]
;
Tropical crops
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In vitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott.) were successfully cryopreserved by vitrification. Excised shoot tips precultured on solidified MS supplemented with 0.3 M sucrose and maintained under a 16 h phtoperiod at 25°C for 16 h were loaded with a mixture of 2 M glycerol plus 0.4 M sucrose for 20 min at 25°C. The shoot tips were then sufficiently dehydrated with a highly concentrated vitrification solution (PVS2) for 20 min at 25°C prior to immersion into liquid nitrogen. Successfully vitrified and warmed shoot tips resumed growth within 7 days and developed shoots directly without intermediate callus formation. The average rate of shoot recovery amounted to around 80%, and the vitrification protocol appeared to be very promising for the cryopreservation of taro germplasm.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050285
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