ISSN:
1573-8221
Keywords:
cyclic nucleotides
;
proliferation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The effect of cyclic AMP (cAMP), dibutyryl-cAMP, and theophylline (an inhibitor of phosphodiesterase, the enzyme converting adenosine-3′, 5′-monophosphate into adenosine-5′-monophosphate) on the intensity of proliferation (as reflected in the increase in the nucleic acid content in the culture), DNA synthesis (thymidine-H3 incorporation), and transplantation properties (ability to repopulatein vivo) of leukemic cells of strain L-5178 was studied. The experiments showed that cAMP in a concentration of 0.8 mM inhibits thymidine-H3 incorporation considerably, retards proliferation, and reduces the transplantability of the leukemic cells. Theophylline and dibutyryl-cAMP have comparatively weak ability to inhibit DNA synthesis and the proliferative activity and transplantation properties of the cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00804943
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