ISSN:
1432-2145
Keywords:
Key words Brassica campestris . cDNA cloning . Floral transition . Shoot apex . Vernalization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The vegetative-to-floral transition of Brassica campestris cv. Osome was induced by vernalization. Poly(A)+RNA was isolated from the transition shoot apex after 6 weeks of vernalization, the floral apex after 12 weeks of vernalization and the expanded leaves just before vernalization, and cDNAs were synthesized. These cDNAs were used for subtraction and differential screening to select cDNA preferentially present in the transition and floral apices. Nucleotide sequences of the resulting 14 cDNA clones were determined, and northern blot analysis was carried out on six cDNAs. Two cDNA clones which did not show significant similarity to known genes were shown to be preferentially expressed in the floral apex.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004970050029
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