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  • 1
    Electronic Resource
    Electronic Resource
    Genetic structure in cultivated and wild carrots (Daucus carota L.) revealed by AFLP analysis (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 227-233 
    Details
    ISSN: 1432-2242
    Keywords: Key words Genetic variation ; UPGMA ; AMOVA ; Relationships ; Crop-wild introgression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Genetic variation within and among five Danish populations of wild carrot and five cultivated varieties was investigated using amplified fragment length polymorphism (AFLP). Ten AFLP primer combinations produced 116 polymorphic bands. Based on the marker data an UPGMA-cluster analysis and principal component analysis (PCA) separated the Daucus collections into three groups, consisting of the wild populations, the old varieties, and the recently bred varieties. The genetic distance between the wild populations reflected the physical distance between collection sites. Analysis of genetic diversity showed that the old varieties released between 1974 and 1976 were more heterogeneous than the newly developed F1 hybrid varieties. The analysis of molecular variation (AMOVA) showed that the major part of the genetic variation in the plant material was found within populations/varieties. The presence of markers specific to the cultivated carrot makes it possible to detect introgression from cultivated to wild types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051473
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  • 2
    Electronic Resource
    Electronic Resource
    Karyotype analysis of regenerated plants from callus cultures of interspecific hybrids of cultivated barley (Hordeum vulgare L.) (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 343-351 
    Details
    ISSN: 1432-2242
    Keywords: H. vulgare ; Interspecific hybrids ; Regenerants ; Chromosome changes ; C-banding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The karyotype of 82 regenerated plants from callus cultures of interspecific hybrids between cultivated barley (Hordeum vulgare L.) and seven polyploid wild barley species was examined by C-banding or Feulgen staining. The karyotypic changes observed in 46 plants included aneuploidy, double haploidy, amphidiploidy, deletions, inversions, extra C-bands, and extra euchromatic segments. Apparently, chromosome 5, 6, and 7 of H. vulgare were more frequently exposed to elimination or structural change than the other chromosomes of this species. Irradiation of calli seemed to enhance the occurrence of karyotypic variants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305826
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  • 3
    Electronic Resource
    Electronic Resource
    Inheritance of oilseed rape (Brassica napus) RAPD markers in a backcross progeny with Brassica campestris (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 492-497 
    Details
    ISSN: 1432-2242
    Keywords: Key words  Interspecific hybridisation ; Introgression ; Gene flow ; Risk assessment ; Transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   Different cultivars/transgenic lines of oilseed rape (Brassica napus) were crossed (as females) with different cultivars/populations of Brassica campestris. All cross combinations produced seed, with an average seed set per pollination of 9.8. Backcrossing of selected interspecific hybrids (as females) to B. campestris resulted in a much lower seed set, average 0.7 seed per pollination. In the single backcross progeny where a large enough population (92 plants) was obtained for analysis, 33 B. napus-specific RAPD markers were investigated to determine the extent of transfer of oilseed rape genetic material into this population. Markers were transferred to the backcross generation with frequencies ranging from 26% to 91%. Almost all of the markers (30/33) were transferred in a frequency not significantly different from 50%. Analysis of the pairwise segregation of markers revealed that 23 markers could be assigned to six linkage groups, most probably reflecting six B. napus C-chromosomes. The presence of backcross plants with recombinant genotypes suggests that complex genetic processes can take place during interspecific hybridisation and backcrossing in these Brassica species. The implications of our results for the possible choice of integration sites of transgenes in oilseed rape are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050154
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  • 4
    Electronic Resource
    Electronic Resource
    Inheritance of rapeseed (Brassica napus)-specific RAPD markers and a transgene in the cross B. juncea x (B. juncea x B. napus) (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 236-241 
    Details
    ISSN: 1432-2242
    Keywords: Brassicaceae ; Interspecific hybridization ; Synteny groups ; Risk assessment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have examined the inheritance of 20 rapeseed (Brassica napus)-specific RAPD (randomly amplified polymorphic DNA) markers from transgenic, herbicide-tolerant rapeseed in 54 plants of the BC1 generation from the cross B. junceax(B. junceaxB. napus). Hybridization between B. juncea and B. napus, with B. juncea as the female parent, was successful both in controlled crosses and spontaneously in the field. The controlled backcrossing of selected hybrids to B. juncea, again with B. juncea as the female parent, also resulted in many seeds. The BC1 plants contained from 0 to 20 of the rapeseed RAPD markers, and the frequency of inheritance of individual RAPD markers ranged from 19% to 93%. The transgene was found in 52% of the plants analyzed. Five synteny groups of RAPD markers were identified. In the hybrids pollen fertility was 0–28%. The hybrids with the highest pollen fertility were selected as male parents for backcrossing, and pollen fertility in the BC1 plants was improved (24–90%) compared to that of the hybrids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220883
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  • 5
    Electronic Resource
    Electronic Resource
    Inheritance of oilseed rape (Brassica napus) RAPD markers in a backcross progeny with Brassica campestris (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 492-497 
    Details
    ISSN: 1432-2242
    Keywords: Interspecific hybridisation ; Introgression ; Gene flow ; Risk assessment ; Transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Different cultivars/transgenic lines of oilseed rape (Brassica napus) were crossed (as females) with different cultivars/populations of Brassica campestris. All cross combinations produced seed, with an average seed set per pollination of 9.8. Backcrossing of selected interspecific hybrids (as females) to B. campestris resulted in a much lower seed set, average 0.7 seed per pollination. In the single backcross progeny where a large enough population (92 plants) was obtained for analysis, 33 B. napus specific RAPD markers were investigated to determine the extent of transfer of oilseed rape genetic material into this population. Markers were transferred to the backcross generation with frequencies ranging from 26% to 91%. Almost all of the markers (30/33) were transferred in a frequency not significantly different from 50%. Analysis of the pairwise segregation of markers revealed that 23 markers could be assigned to six linkage groups, most probably reflecting six B. napus C-chromosomes. The presence of backcross plants with recombinant genotypes suggests that complex genetic processes can take place during interspecific hybridisation and backcrossing in these Brassica species. The implications of our results for the possible choice of integration sites of transgenes in oilseed rape are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223698
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  • 6
    Electronic Resource
    Electronic Resource
    Production and cytogenetics of Brassica campestris-alboglabra chromosome addition lines (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 633-640 
    Details
    ISSN: 1432-2242
    Keywords: Key words Brassica species ; Addition line ; RAPD marker ; Meiotic pairing ; Intergenomic homoeology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Four different Brassica campestris-alboglabra monosomic addition lines (AA+1 chromosome from C, 2n=21) were obtained after consecutive backcrosses between resynthesized B. napus (AACC, 2n=38) and the parental B. campestris (AA, 2n=20) accession. The alien chromosomes of B. alboglabra (CC, 2n=18) in the addition lines were distinguished by random amplified polymorphic DNA (RAPD) marker analysis and morphology of mitotic chromosomes. Four RAPD marker synteny groups were established, which represented the four different alien chromosomes of B. alboglabra in the four addition lines. Three of the four addition lines were identified to harbour chromosomes 4, 8 or 9 of B. alboglabra. Studies on meiotic pairing in the addition lines revealed intergenomic homoeology relationships among specific chromosome arms between the A- and C-genomes. The long arm of B. campestris chromosome 9 was homoeologous with the long arm of B. alboglabra chromosome 4, while its short arm with the short arms of B. alboglabra chromosomes 8 and 9. Such an intergenomic homoeology relationship supports the hypothesis that B. campestris and B. alboglabra share a common ancestor but that chromosomal rearrangements have occurred during the evolution of the two species. Intergenomic introgression was observed in the progenies of the addition lines. The introgression of an entire B. alboglabra marker synteny group into the B. campestris genome implied the possible occurrence of interspecific chromosomal substitution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050460
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  • 7
    Electronic Resource
    Electronic Resource
    Random amplified polymorphic DNA markers of theBrassica alboglabra chromosome of aB. campestris-alboglabra addition line (1996)
    Springer
    Chromosome research 4 (1996), S. 111-114 
    Details
    ISSN: 1573-6849
    Keywords: addition line ; erucic acid ; flower colour ; intergenomic recombination ; leucine aminopeptidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The alien C-genome chromosome in aBrassica campestris-alboglabra monosomic addition line was characterized by random amplified polymorphic DNA (RAPD) analysis. The alien chromosome carried three loci, Ec, Wc and Lap-1C, controlling synthesis of erucic acid, white flower colour and a fast-migrating band of leucine aminopeptidase (Lap-1Cc) respectively. The RAPD analysis revealed 17 markers specific to the alien chromosome. Among 45 offspring plants from the selfed addition line the alien C-chromosome was transmitted to 15 plants, four plants had only parts of this chromosome and the remaining 26 plants did not carry the chromosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02259703
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  • 8
    Electronic Resource
    Electronic Resource
    High capacity of plant regeneration from callus of interspecific hybrids with cultivated barley (Hordeum vulgare L.) (1986)
    Springer
    Plant cell, tissue and organ culture 6 (1986), S. 199-207 
    Details
    ISSN: 1573-5044
    Keywords: Barley ; callus ; Hordeum ; interspecific hybrids ; plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus was induced from hybrids between cultivated barley (Hordeum vulgare L. ssp. vulgare) and ten species of wild barley (Hordeum L.) as well as from one backcross line ((H. lechleri x H. vulgare) x H. vulgare). Successful callus induction and regeneration of plants were achieved from explants of young spikes on the barley medium J 25–8. The capacity for plant regeneration was dependent on the wild parental species. In particular, combinations with four related wild species, viz. H. jubatum, H. roshevitzii, H. lechleri, and H. procerum, regenerated high numbers of plants from calli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040005
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