ISSN:
1365-2133
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Anthralin is a most widely used compound for topical treatment of psoriasis. Whereas numerous studies have ascertained anthralin as a safe and effective drug its mode of action still remains unclear. Previous studies demonstrated dose-dependent inhibition of a number of pro-inflammatory functions in human enutrophils and monocytes (MO). The aim of the present study was to investigate in stimulated MO the effect of anthralin on the secretion of cytokines which are of known importance for the psoriatic tissue reaction.Highly purifies MO were incubated with anthralin (0·01–1·0μg/ml), its clinically inactive derivative danthrone (0·1 and 10 μg/ml), the solvent acetone, or medium alone. Culture supernatants were analysed for immunoreactivity for interleuking-1β, and -8 (IL-1β, IL-8), and tumour necrosis factor β (TNF-β) by specific ILISA. IL-6 bioactivity was determined using the B9-bioassay. Additionally, IL-1 bioactivity was measured by the D10[N4]M-bioassay.The results show a dose-dependent inhibition of MO IL-6, IL-8, and TNF-α release with a half- maximal inhibitory concentration of 0.25–0.6μ/ml of anthralin. There was no effect of danthrone or acetone on the secretion of these cytokines from MO. Secretion of IL-1β immunoreactivity measured by ELISA as determination of biological activity of IL-1 using the D10[N4]M- bioassay revealed a slight increase in IL-1 secretion with a maximum at an anthralin concentration of 0.1 μg/ml. Danthrone at a concentration of 10μg/ml and acetone (0.1%) similarly enhanced IL-1 secretion from human MO measured by both methods.Our results demonstrate a differential, dose-dependent inhibition of cytokine secretion from human MO by anthralin. The present data provide evidence that the anti-inflammatory and anti- proliferative activity of anthralin may at least in part be due to its inhibitory effect on pro-inflammatory cytokine secretion by MO.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1365-2133.1997.d01-1232.x
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