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  • 1
    Electronic Resource
    Electronic Resource
    Soluble auxin-binding proteins in pea epicotyls (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 56 (1982), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Auxin-binding proteins, have been identified in the soluble cytoplasrnic protein fraction of etiolated pea epicotyls, Pisum sativum L., cv. “Dippes Gelbe Victoria”. The binding is specific for the auxins NAA, IAA and 2,4-D with a KD in the range of 0.1–0.4 μM. Moreover, the binding is competitive, sensitive to digestion by proteinase and shows linearity with the protein content of the assay mixture. The binding proteins appear to be very labile, since repeated freezing and thawing destroys specific binding. No clear pH-optimum could be detected in the physiological pH-range 5.5–8.0, but the binding was doubled at pH 8.0 compared to pH 5.5–7.0.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1982.tb00319.x
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  • 2
    Electronic Resource
    Electronic Resource
    Agrobacterium tumefaciens-mediated transformation of Pisum sativum L. using binary and cointegrate vectors (1990)
    Springer
    Plant cell reports 9 (1990), S. 276-279 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Epicotyl segments and nodus expiants from etiolated seedlings of Pisum sativum were transformed using Agrobacterium tumefaciens strains GV 2260 (p35S GUS INT) and GV 3850 HPT carrying either a neomycin- or hygromycinphosphotransferase-gene as selectable markers. The transgenic character of hygromycin- or kananamycin-resistant tissue was confirmed by detection of nopaline or neomycinphosphotransferase-II- and ß-glucuronidase activity in crude extracts of resistant tissues. Up to 5 % of developing shoots from shoot proliferating nodi were regenerated via organogenesis to kanamycin-resistant plantlets. Transformation frequency in vitro was found to be influenced by expiant source, A. tumefaciens strain, pea genotype and duration of cocultivation. Acetosyringone did not increase the transformation rate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232301
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  • 3
    Electronic Resource
    Electronic Resource
    Physiological and biochemical characterization of glyoxalase I, a general marker for cell proliferation, from a soybean cell suspension (1993)
    Springer
    Planta 189 (1993), S. 561-566 
    Details
    ISSN: 1432-2048
    Keywords: Auxin ; Enzyme induction ; Glycine (cell suspension) ; Glyoxalase I (purification)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using a strictly auxin-dependent soybean (Glycine max (L.) Merr.) cell suspension, we studied the correlation of auxin-dependent cell proliferation and the activity of glyoxalase I (S-lactoylglutathione-lyase EC 4.4.1.5.), an enzyme generally associated with cell proliferation in animal, microbial and, as reported recently, also plant systems. We found the activity of glyoxalase I to be modulated during the proliferation cycle, with a maximal activity between day 2 and day 4 of culture growth. After starving the culture of auxins for three subsequent periods, both the enzyme activity and cell growth could be re-initiated with auxin. Enzyme activity reached its maximum 1 d before cell number was at a maximum. The enzyme was purified to homogeneity and characterized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00198220
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  • 4
    Electronic Resource
    Electronic Resource
    Thidiazuron-induced high frequency of shoot induction and plant regeneration in protoplast derived pea callus (1995)
    Springer
    Plant cell reports 15 (1995), S. 26-29 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protoplasts isolated from lateral shoot buds of cotyledon-free pea embryo axes were regenerated to callus. Protoplast derived calluses with a diameter of about 1cm were transferred to shoot induction media, containing different concentrations (1–50µM) of thidiazuron. Shoot formation was observed after 16 weeks up to 12% efficiency. Thidiazuron (10µM) was the most effective concentration in all experiments. Shoot buds elongated in medium supplemented with N-isopentenyl adenine and indole-3-butyric acid. Since rooting was almost impossible in these thidiazuron-induced shoots, shoots were grafted onto young pea seedlings and regenerated to fertile plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01690247
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  • 5
    Electronic Resource
    Electronic Resource
    Plant regeneration from pea protoplasts via somatic embyogenesis (1989)
    Springer
    Plant cell reports 8 (1989), S. 379-382 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plant regeneration via somatic embryogenesis was obtained from pea protoplasts. Strong auxins (picloram or 2.4-D) and increased osmolarity of the medium were necessary for embryo induction. Relatively high amounts of embryogenic calli could be obtained in 2 genotypes. After a period on hormone-free medium, a second induction of somatic embryos was possible. Further development of somatic embryos was accomplished on GA3 — containing medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270073
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  • 6
    Electronic Resource
    Electronic Resource
    Marker proteins for embryogenic differentiation patterns in pea callus (1987)
    Springer
    Plant cell reports 6 (1987), S. 50-54 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polypeptide pattern alterations during somatic embryogenesis were investigated using callus cultures of two Pisum sativum genotypes. Both genotypes show the formation of two different callus lines from the same explant after six to eight weeks in culture: a nodular yellowish callus line, which forms somatic embryoids in suspension cultures (e+) and a white compact callus line with no regenerative capacity (e−). The cytosol proteins of the two different callus lines were separated in a semi-preparative two-dimensional system and the polypeptide patterns were compared. Two protein bands were found (P1: Mr=45000 D, pI=7.0–7.1; P2: Mr=7000 D, pI=〈4.5), which were characteristic of the putatively embryogenic (e+) callus line in all tissues investigated (two genotypes × two explant sources). These proteins found in nodular (e+) pea cultures are very similar to two proteins found in Daucus carota suspension cultures preceding the formation of somatic embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269738
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  • 7
    Electronic Resource
    Electronic Resource
    Modulation of soluble auxin-binding proteins in soybean cell suspensions (1988)
    Springer
    Plant cell reports 7 (1988), S. 178-181 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rapidly growing cell suspensions of soybean were analyzed for the presence of cytoplasmic high-affinity binding sites for auxin. Cytosol preparations were studied in lag, log and early stationary phase of the growth cycle. Two binding sites were detected, which show some similarities with binding sites previously reported from etiolated pea epicotyls. While the number of both sites declined in the cytoplasm during the growth cycle, the number of one of the two sites increased at the onset of rapid cell divisions. In parallel, both sites exhibited an increase in binding affinity during the growth cycle. The data will be discussed in relation to other reports on soluble auxin binding as well as to possible physiological functions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269317
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  • 8
    Electronic Resource
    Electronic Resource
    Indole-3-lactic acid is a weak auxin analogue but not an anti-auxin (1995)
    Springer
    Journal of plant growth regulation 14 (1995), S. 191-197 
    Details
    ISSN: 1435-8107
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Indole-3-lactic acid (ILA) is a naturally occurring indole derivative, preferably detected in soil bacteria and fungi and only in low amounts in plants. T-DNA gene 5 of Agrobacterium tumefaciens was found to be involved in the synthesis of ILA in transformed plant tissues, but the physiologic relevance for ILA production in plants is unclear. The related molecular structure of ILA to the natural auxin indole-3-acetic acid (IAA) makes ILA a good candidate for an auxin analogue. We examined the possible auxin activity of ILA on elongation, proliferation, and differentiation in Pisum sativum L. Results presented in this paper indicate that there are no or only weak effects of ILA toward the activity of auxins when used in the physiologic concentration range. Furthermore, no antagonistic effects of ILA were found. Biochemical analysis using the equilibrium dialysis binding system resulted in no high affinity ILA binding to an enriched protein fraction containing auxin-binding protein (ABP44), whereas 1-naphthaleneacetic acid exhibited high affinity auxin binding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00204911
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  • 9
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis from pea embryos and shoot apices (1990)
    Springer
    Plant cell, tissue and organ culture 20 (1990), S. 7-14 
    Details
    ISSN: 1573-5044
    Keywords: genotype ; plant growth regulators ; Pisum sativum ; plant regeneration ; somatic embryogenesis ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Conditions were defined for plant regeneration via somatic embryogenesis in pea, using explants from immature zygotic embryos or from shoot apices. For the induction of somatic embryos, an auxin (picloram or 2,4-dichlorophenoxyacetic acid) was required. Embryogenic callus originated from embryonic axis tissue of immature embryos and from the axillary-bud region and the plumula of shoot apices. A clear effect of embryo size on somatic embryogenesis was shown. There were differences in frequency of somatic embryogenesis among the five genotypes used in the study. Additions of BA to auxin-containing medium reduced embryo production. Histological examinations confirmed the embryogenic nature of the immature embryo cultures and revealed that somatic embryos originated from the meristematic areas near the callus surface.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034751
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  • 10
    Electronic Resource
    Electronic Resource
    Induction of somatic embryos in pea, Pisum sativum L. (1984)
    Springer
    Plant cell, tissue and organ culture 3 (1984), S. 319-324 
    Details
    ISSN: 1573-5044
    Keywords: Somatic embryogenesis ; germination ; root formation ; Pisum sativum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using low concentrations of picloram (0.06 mg/l), embryoids were formed on the surface of leaf-derived callus of pea, Pisum sativum L. (c.v. Dippes Gelbe Victoria) upon transfer to liquid medium. After some days in culture, embryoids spontaneously separated from the calli, and developed into torpedo-shaped embryos, which were transferred to solid medium. In a second series of experiments, embryos were also formed by mutant 489C and a genetic line of Pisum arvense, which additionally exhibited embryogenesis also from epicotyl-derived callus. Some of the embryos showed root formation, but no shoot morphogenesis occurred. In a limited number of cases, an additional root was formed in the apparent shoot apical region after 2–5 days.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043083
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