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  • 1
    Electronic Resource
    Electronic Resource
    THE GENETICS OF HYPHAL FUSION AND VEGETATIVE INCOMPATIBILITY IN FILAMENTOUS ASCOMYCETE FUNGI (2000)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Genetics 34 (2000), S. 165-186 
    Details
    ISSN: 0066-4197
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Notes: Abstract Filamentous fungi grow as a multicellular, multinuclear network of filament-shaped cells called hyphae. A fungal individual can be viewed as a fluid, dynamic system that is characterized by hyphal tip growth, branching, and hyphal fusion (anastomosis). Hyphal anastomosis is especially important in such nonlinear systems for the purposes of communication and homeostasis. Filamentous fungi can also undergo hyphal fusion with different individuals to form heterokaryons. However, the viability of such heterokaryons is dependent upon genetic constitution at heterokaryon incompatibility (het) loci. If hyphal fusion occurs between strains that differ in allelic specificity at het loci, vegetative incompatibility, which is characterized by hyphal compartmentation and cell lysis, is induced. This review covers microscopic and genetic analysis of hyphal fusion and the molecular and genetic analysis of the consequence of hyphal fusion between individuals that differ in specificity at het loci in filamentous ascomycetes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.genet.34.1.165
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A kalilo-like linear plasmid in Louisiana field isolates of the pseudohomothallic fungus Neurospora tetrasperma (1994)
    Springer
    Current genetics 26 (1994), S. 336-343 
    Details
    ISSN: 1432-0983
    Keywords: Kalilo ; Plasmid ; Senescence ; Neurospora
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two Louisiana strains of Neurospora tetrasperma contain a linear plasmid (LA-kalDNA) with a restriction map identical to the Hawaiian Neurospora intermedia senescence plasmid, kalDNA, but with termini 100 nucleotide pairs shorter. One of these strains also bore a circular plasmid similar to the Hawaiian circular plasmid Hanalei-2. One species probably acquired both plasmids from the other by horizontal transfer, at a time sufficiently distant for sequence divergence to take place. Many LA-kalDNA-bearing derivative strains senesced, but this plasmid does not guarantee senescence. Furthermore, LA-kalDNA does not insert into mtDNA. One senescent strain showed no LA-kalDNA. The plasmids are effectively transmitted via the pseudohomothallic sexual cycle. Single mating-type derivatives transmit plasmids maternally.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310498
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of Neurospora crassa mutants isolated following repeat-induced point mutation of the beta subunit of fatty acid synthase (1999)
    Springer
    Current genetics 36 (1999), S. 147-152 
    Details
    ISSN: 1432-0983
    Keywords: Key words Fatty acid biosynthesis ; Filamentous fungi ; Neurospora crassa ; Repeat-induced point mutation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Neurospora crassacel-2 mutants were isolated following repeat-induced point mutation using part of the gene encoding β-fatty acid synthase. These mutants are phenotypically less leaky than cel-1, which has a defective α-fatty acid synthase. The cel-2 mutant had a strict fatty acid (16:0) requirement for growth, and syn-thesized less fatty acid de novo than cel-1. Unlike cel-1, cel-2 has impaired fertility, and homozygous crosses are infertile, suggesting a low but strict requirement for fatty acid synthesis during sexual development. Like cel-1, cel-2 synthesized unusually high levels of the polyunsaturate 18:3Δ9,12,15, and elongated 18:2Δ9,12 and 18:3Δ9,12,15 to 20:2Δ11,14 and 20:3Δ11,14,17, respectively. These fatty acids are not synthesized by wild-type, except following treatment with cerulenin (a fatty acid synthase inhibitor), demonstrating that inhibition of fatty acid biosynthesis results in a relative increase in both fatty acid desaturation and elongation activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050484
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    Paper (German National Licenses)
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