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  • 1
    Electronic Resource
    Electronic Resource
    Amino acid-dependent regulation of the Saccharomyces cerevisiaeGSH1 gene by hydrogen peroxide (1997)
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 23 (1997), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The tripeptide γ-l-glutamyl-l-cysteinylglycine (glutathione) is one of the major antioxidant molecules of cells and plays a vital role in buffering the cell against reactive oxygen species and toxic electrophiles. In the yeast Saccharomyces cerevisiae, the first enzyme involved in glutathione biosynthesis, γ-glutamylcysteine synthetase, is encoded by the GSH1 gene. This study shows that the regulation of the yeast GSH1 gene by oxidants and the heavy metal cadmium is at the level of transcription. We also demonstrate that the regulation of the GSH1 gene by H2O2 depends upon the presence of the amino acids glutamate, glutamine and lysine in the media. Moreover, regulation of the GSH1 gene by H2O2, although requiring the Yap1 protein, appears to be mediated by a mechanism distinct from that which regulates the Yap1-dependent induction of genes encoding thioredoxin (TRX2) and a stress-inducible HSP70 (SSA1) by H2O2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1997.2081572.x
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  • 2
    Electronic Resource
    Electronic Resource
    The role of the YAP1 and YAP2 genes in the regulation of the adaptive oxidative stress responses of Saccharomyces cerevisiae (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 16 (1995), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The YAP1 and YAP2 genes encode yeast transcription factors of the c-jun family. We show that yeast mutants deleted for either the YAP1 or the YAP2 genes are hypersensitive to oxidants, particularly H2O2, and that these genes play a role in regulating the induction of the H2O2 adaptive stress response in Saccharomyces cerevisiae. They do not significantly affect the regulation of the superoxide adaptive stress response. The intrinsic resistance of stationary-phase and respiring yeast cells towards superoxide anions is unaffected by deletion of the YAP1 and YAP2 genes. However, resistance towards H2O2 under these conditions is significantly reduced. We show that expression of the yeast GSH1 gene (encoding γ-glutamylcysteine synthetase) and the SSA1 gene (encoding an HSP70 isoform) are induced by oxidants. Unlike the SSA1 and thioredoxin (TRX2) genes, expression of the GSH1 gene is more strongly induced by superoxide anions than by H2O2. In the absence of added oxidants, transcription of the GSH1 gene is reduced in strains carrying the yap1 deletion. However, we show that Yap1 is not required for the superoxide anion-mediated induction of GSH1 gene expression. Furthermore, while the H2O2-mediated induction of SSA1 expression is shown to be YAP1 dependent, the heat-shock-mediated induction of the SSA1 gene does not require YAP1. We also present evidence to show that the YAP2 gene does not regulate the expression of the TRX2, SSA1 or GSH1 genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1995.tb02407.x
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of the adaptive oxidative stress response of Candida albicans (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 138 (1996), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Treatment of Candida albicans with low concentrations of either hydrogen peroxide or menadione (a Superoxide generating agent) induces an adaptive response which protects cells from the lethal effects of a subsequent challenge with higher concentrations of these oxidants. Pre-treatment with either menadione or hydrogen peroxide is protective against cell killing by either oxidant. This suggests that the pathogenic yeast C. albicans (unlike the budding yeast Saccharomyces cerevisiae which has separate responses) possesses an adaptive response that responds to both these oxidants. In addition, we found that C. albicans showed a greater level of resistance to oxidants, both H2O2 and redox-cycling agents, compared to that observed with S. cerevisiae. In an attempt to characterise the oxidative stress response in more detail we have analysed the effect of oxidants on the activities of a number of enzymes with known antioxidant activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08139.x
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  • 4
    Electronic Resource
    Electronic Resource
    Glutathione is an important antioxidant molecule in the yeast Saccharomyces cerevisiae (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 141 (1996), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The tripeptide γ-l-glutamyl-l-cystinylglycine (glutathione) is one of the major antioxidant molecules of cells and is thought to play a vital role in buffering the cell against reactive oxygen species and toxic electrophiles. We wished to determine the role of glutathione in the protection of the yeast Saccharomyces cerevisiae against oxidative stress. This study shows that glutathione is an important antioxidant molecule in yeast, with γ-glutamylcysteine synthetase (gshI) mutants, deficient in glutathione synthesis, being hypersensitive to H2O2 and Superoxide anions in both exponential- and stationary-phase cultures. Despite this, these mutants are still able to induce adaptive stress responses to oxidants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08386.x
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  • 5
    Electronic Resource
    Electronic Resource
    A suppressor of a yeast splicing mutation ( prp8-l ) encodes a putative ATP-dependent RNA helicase (1991)
    [s.l.] : Nature Publishing Group
    Nature 349 (1991), S. 715-717 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] To identify genes encoding splicing factors that interact with the PRP8 protein, we isolated extragenic suppressors ofaprp8-l mutation. We sought suppressors that can not only suppress a prp8-l mutation but also render the growth of the cells cold-sensitive, as this would greatly aid the analysis ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/349715a0
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  • 6
    Electronic Resource
    Electronic Resource
    Protein interactions in nuclear pre-mRNA splicing in Saccharomyces cerevisiae (1990)
    Springer
    Molecular biology reports 14 (1990), S. 141-142 
    Details
    ISSN: 1573-4978
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00360449
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  • 7
    Electronic Resource
    Electronic Resource
    Oxidative stress responses of the yeast Saccharomyces cerevisiae (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 14 (1998), S. 1511-1527 
    Details
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; oxidative stress ; stress response ; signal transduction ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: All aerobically growing organisms suffer exposure to oxidative stress, caused by partially reduced forms of molecular oxygen, known as reactive oxygen species (ROS). These are highly reactive and capable of damaging cellular constituents such as DNA, lipids and proteins. Consequently, cells from many different organisms have evolved mechanisms to protect their components against ROS. This review concentrates on the oxidant defence systems of the budding yeast Saccharomyces cerevisiae , which appears to have a number of inducible adaptive stress responses to oxidants, such as H2 O2 , superoxide anion and lipid peroxidation products. The oxidative stress responses appear to be regulated, at least in part, at the level of transcription and there is considerable overlap between them and many diverse stress responses, allowing the yeast cell to integrate its response towards environmental stress. © 1998 John Wiley & Sons, Ltd.
    Type of Medium: Electronic Resource
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