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  • 1
    Electronic Resource
    Electronic Resource
    The influence of a residual group in low-molecular-weight allergoids of Artemisia vulgaris pollen on their allergenicity, IgE- and IgG-binding properties (2002)
    Oxford, UK : Munksgaard International Publishers
    Allergy 57 (2002), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background: Reaction of ε-amino groups of lysine with potassium cyanate, maleic, or succinic anhydride leads to allergoids of low molecular weight. No study has been performed to compare their properties and investigate the influence of a residual group on allergenicity and human IgE- and IgG-binding of these derivatives.Methods: Allergoids of a pollen extract of Artemisia vulgaris were obtained by means of potassium cyanate, and succinic and maleic anhydride. Biochemical properties were investigated by determination of amino groups, enzyme activity, isoelectric focusing IEF and SDS–PAGE. IgE- and IgG-binding was determined using immunoblots and ELISA inhibition. Allergenicity was investigated by skin prick tests (SPT) on a group of 52 patients, of which 6 were control subjects, 30 were patients with no previous immunotherapy (IT), and 16 were patients undergoing immunotherapy.Results: The same degree of amino-group modification (more than 85%), residual enzyme activity (less then 15%), IEF, and SDS–PAGE pattern were noted. In the immunoblots of IgE-binding, there was more pronounced reduction in the succinyl and maleyl derivatives than in the carbamyl one. IgG-binding was less affected by carbamylation than by acid anhydride modification. The SPT showed that the succinylated derivative had the most reduced allergenicity (98% showed a reduced wheal diameter when tested with the succinyl derivative, 87% with the maleyl allergoid, and 83% with the carbamyl allergoid). The most significant difference among allergoids could be seen in the group of patients with high skin reactivity (83% of patients showed no reaction to the succinyl derivative when compared to the value of 28% for the carbamyl derivative or 22% for the maleyl derivative).Conclusions: According to our results, all three modification procedures yielded allergoids with a similar extent of modification. No single biochemical parameter investigated in the study could predict the degree of reduced allergenicity in vivo. The most reduced allergenicity was seen in the succinyl derivative while the preservation of IgG binding epitopes was of the highest degree for the carbamyl derivative.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1398-9995.2002.23739.x
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  • 2
    Electronic Resource
    Electronic Resource
    Food allergy to pork meat (2002)
    Oxford, UK : Munksgaard International Publishers
    Allergy 57 (2002), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1398-9995.2002.23832_5.x
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  • 3
    Electronic Resource
    Electronic Resource
    Comparison of allergenic potentials of timothy (Phleum pratense) pollens from different pollen seasons collected in the Belgrade area (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 52 (1997), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We investigated extracts of timothy grass pollen from four seasons (1989, 1990, 1991, and 1994) by protein content, SDS-PAGE, immunoblot, RAST, RAST inhibition, and crossed immunoelectrophoresis. Extract of the pollen from 1991 showed the lowest yield in quantitative assays. SDS-PAGE, crossed immunoelectrophoresis, RAST, and RAST inhibition expressed approximately comparable patterns for all extracts except that from 1991. Obviously, the quality of grass pollens, as shown for some ragweed (Ambrosia elatior) pollens depend on year of collection. Our findings are partially in agreement with some earlier examinations of the quality of timothy pollen from different pollen seasons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1997.tb00977.x
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  • 4
    Electronic Resource
    Electronic Resource
    Crystal structure of usnic acid sodium salt 2 1/2 hydrate (1993)
    Springer
    Journal of chemical crystallography 23 (1993), S. 107-111 
    Details
    ISSN: 1572-8854
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Usnic acid, 1,3(2H,9bH)-dibenzofurandione-2,6-diacetyl-7,9-dihydroxy-8,9b-dimethyl, (1) was isolated from the lichenUsnea barbata by supercritical CO2 extraction. The corresponding sodium salt 2 1/2 hydrate was obtained by reacting the acid with sodium carbonate. (C18H15O7Na·2.5 H2O) × 2,M r =822.68, triclinic,P1,a=14.179(8),b=7.369(4),c=9.109(6)Å,α=88.47(2),β=105.35(2),γ=92.27(2)°,V=917(2)Å3,Z=1,D x =1.490 g cm−3, λ(MoKα)=0.71073 Å,μ=1.32 cm−1,F(000)=430,T=293(2)K. The structure was solved by direct methods and refined toR=0.038 for 3229 observed reflections. Both Na ions are pentacoordinated. Their coordination polyhedra are distorted square pyramids bound together by a common water molecule (OW1). The molecules are also joined together by a common water oxygen (OW2) in an infinite chain which lies parallel to theb-axis. The chains are held together by hydrogen bonds. The conformation of usnic acid in the complex differs from that found previously by a rotation of the acetyl group bound to the cyclohexadienone ring.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01195444
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  • 5
    Electronic Resource
    Electronic Resource
    NMR studies and X-ray crystallography of galdosol 5-methyl ether from Salvia officinalis L. (1992)
    Springer
    Journal of chemical crystallography 22 (1992), S. 585-590 
    Details
    ISSN: 1572-8854
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The title compound 2H-10,4a-(epoxy-methano)-phenantren-12-one-l,3,4,9,10,10a-hexahydro-6-hydroxy-5-methoxy-9-keto-1,1-dimethyl-7-(1-methylethyl) isolated fromSalvia officinalis L. have been investigated by nuclear magnetic resonance (NMR) spectroscopy and X-ray analysis. Investigation of1H and13C NMR, homonuclear (COSY) (1H-1H) and heteronuclear (1H-13C) spectra, permitted the unambiguous assignment of the1H and13C spectra, what gave the possibility to resolve the signals of similar natural products. Crystal data are: C21H26O5,M r=358.43, orthorhombic,P21,21,21, (No. 19),a=10.671(2),b=11.976(3),c=14.601(5)Å,V=1865.9(9)Å3,Z=4,D x=1.276 mg−3, λ(MoKα)=0.7107 Å,μ=0.84 cm−1, F(000)=768,T=293(1) K. The structure was solved by direct methods and refined toR=0.041 for 1832 observed reflections collected on CAD-4 diffractometer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01161344
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  • 6
    Electronic Resource
    Electronic Resource
    Blood-brain barrier permeability changes during acute allergic encephalomyelitis induced in the guinea pig (1989)
    Springer
    Metabolic brain disease 4 (1989), S. 33-40 
    Details
    ISSN: 1573-7365
    Keywords: experimental allergic encephalomyelitis (EAE) ; blood-brain barrier ; guinea pig ; immunoglobulin G
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Blood-brain barrier permeability to homologous serum125I-IgG and to D-[3H]mannitol was studied by means of the brain vascular perfusion method in guinea pigs with experimental allergic encephalomyelitis (EAE). EAE was induced with homologous myelin basic protein (MBP) after pretreatment with foreign protein and muramyl dipeptide (MDP). The results suggest a significant comparable increase in IgG blood-to-brain clearance in the parietal cortex, hippocampus, and caudate nucleus, during vascular perfusion of the brains of animals, after 7 and 20 days of EAE. On the other hand, unidirectional transfer of mannitol in the same period of EAE was markedly augmented only in the hippocampus, but no significant changes in the parietal cortex or caudate nucleus were observed. Cerebrospinal fluid (CSF)/serum ratios for IgG and albumin were both significantly increased, suggesting an increase in blood-CSF barrier permeability, but more for albumin than for IgG. The results were confirmed by immunohistochemical determination of the IgG deposits in the brains of EAE animals, during vascular perfusion with unlabeled homologous IgG. An important role of the blood-brain barrier for the central nervous system immunoglobulin homeostasis during EAE is suggested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00999491
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  • 7
    Electronic Resource
    Electronic Resource
    Development of RAST assay for determination of anti-Populus canadensis IgE antibodies (1996)
    Springer
    Journal of radioanalytical and nuclear chemistry 206 (1996), S. 145-149 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract One of the frequent causes of pollen allergy in our region (Serbia, Yugoslavia) is the pollen of poplar (Populus canadensis). The aim of this study was to form RAST for the determination of specific anti-Populus canadensis IgE antibodies. Affinity purified and radiolabelled (I-125) MoAb E1 was used for forming assay for the determination of specific IgE. By titration of extract of poplarPopulus canadensis we determined that the quantity of 0.65 mL extract is needed for coupling of lg BrCN activated paper discs. Coupling was performed in Na2CO3/NaHCO3 buffer pH 10, on 4°C for 48h. Using this newely formed RAST, specific forPopulus canadensis, we have deteminated anti-Populus canadensis IgE antibodies as well as cross reactivity between pollens ofPopulus canadensis andPopulus deltoides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02040051
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