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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 91 (1995), S. 33-37 
    ISSN: 1432-2242
    Keywords: Arabidopsis thaliana ; Flowering time ; Genotype-by-environment interaction ; Mapping ; Quantitative trait loci
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The interval mapping method is widely used for the genetic mapping of quantitative trait loci (QTLs), though true resolution of quantitative variation into QTLs is hampered with this method. Separation of QTLs is troublesome, because single-QTL is models are fitted. Further, genotype-by-environment interaction, which is of great importance in many quantitative traits, can only be approached by separately analyzing the data collected in multiple environments. Here, we demonstrate for the first time a novel analytic approach (MQM mapping) that accommodates both the mapping of multiple QTLs and genotype-by-environment interaction. MQM mapping is compared to interval mapping in the mapping of QTLs for flowering time in Arabidopsis thaliana under various photoperiod and vernalization conditions.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 85 (1992), S. 252-260 
    ISSN: 1432-2242
    Keywords: EM-algorithm ; Generalised linear model ; Genetic linkage map ; Mixture of distributions ; Molecular marker ; Quantitative trait locus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In a segregating population a quantitative trait may be considered to follow a mixture of (normal) distributions, the mixing proportions being based on Mendelian segregation rules. A general and flexible mixture model is proposed for mapping quantitative trait loci (QTLs) by using molecular markers. A method is discribed to fit the model to data. The model makes it possible to (1) analyse non-normally distributed traits such as lifetimes, counts or percentages in addition to normally distributed traits, (2) reduce environmental variation by taking into account the effects of experimental design factors and interaction between genotype and environment, (3) reduce genotypic variation by taking into account the effects of two or more QTLs simultaneously, (4) carry out a (combined) analysis of different population types, (5) estimate recombination frequencies between markers or use known marker distances, (6) cope with missing marker observations, (7) use markers as covariables in detection and mapping of QTLs, and finally to (8) implement the mapping in standard statistical packages.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2242
    Keywords: Inheritance ; S. arnezii x hondelmannii ; S. berthaultii ; S. leptophyes ; S. microdontum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Resistant genotypes of the diploid tuber-bearing South American species Solarium arnezii x hondelmannii, S. berthaultii, S. leptophyes and S. microdontum were crossed with three diploid genotypes of S. tuberosum that varied in resistance and maturity type. The progenies were field tested for 2 years for resistance to a complex race of Phytophthora infestans. A wealth of genetic variation for resistance was found in most of the progenies. At least two susceptibility groups could be distinguished in some progenies of S. microdontum. This could be explained by the presence of several major resistance genes in the wild parent and, unexpectedly, in the susceptible parent SH 82-44-111. In most of the wild parents and in the susceptible parent SH 77-114-2988 there appeared to be minor resistance genes. General combining ability effects were predominant; small specific combining ability effects were detected in some crosses of S. microdontum. Gene action appeared dominant in some crosses.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 51 (1990), S. 131-140 
    ISSN: 1573-5060
    Keywords: Single seed descent ; number of generations ; number of lines ; level of heterozygosity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Single seed descent is an easy inbreeding strategy aimed at deferring selection in successive generations of a cross between two homozygous lines to that generation for which selection looks most successful. Different selection aims are discussed. For characters that are determined by a small number of (major) genes, numbers of plants and generations are tabulated that guarantee the breeder to achieve his aim with a given level of heterozygosity and a given probability.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5060
    Keywords: Phaseolus vulgaris ; common bean ; growth reduction ; seed weight ; suboptimal temperature ; vegetative phase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A non-destructive method has been developed to select common bean (Phaseolus vulgaris L.) plants whose growth is less effected at a suboptimal temperature. Shoot weight was determined at a suboptimal (14°C) and optimal temperature (20°C), 38 days after sowing and accessions identified with a significantly lower than average weight reduction at 14°C compared to their weight at 20°C. Weight of primary leaves and of the shoot was correlated with seed weight at both temperatures, but no correlation was found between shoot weight reduction at 14°C and seed weight.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-5060
    Keywords: cucumber ; Cucumis sativus ; leaf chlorosis ; powdery mildew ; single seed descent ; Sphaerotheca fuliginea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The genetic relation between powdery mildew resistance and sensitivity for leaf chlorosis of glasshouse cucumber was investigated. The powdery mildew resistant, leaf chlorosis sensitive hybrid variety ‘Profito’ was crossed with the powdery mildew susceptible, non chlorosis sensitive hybrid variety ‘Corona’. Forty four F6 inbred lines of this cross, produced by single seed descent, were tested for powdery mildew resistance (PMR) and leaf chlorosis sensitivity (LCS). PMR and LCS were positively correlated (r=0.59). One or more of the PMR genes probably causes LCS as a pleiotropic effect or is closely linked to LCS, but also other factors, not genetically linked to PMR can cause LCS. Five F6 lines combined a significantly higher level of PMR than ‘Corona’ with a significantly lower LCS than ‘Profito’. Three of these lines could hardly be distinguished from ‘Corona’ for the level of LCS.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-5044
    Keywords: abscisic acid ; Allium cepa ; Allium fistulosum ; Allium porrum ; MS medium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The plant regeneration ability of zygotic embryo-derived callus cultures was studied for 12 A. cepa varieties and accessions, two A. fistulosum varieties, one A. fistulosum x A. cepa interspecific hybrid and two A. porrum varieties. Compact embryogenic callus was induced on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid. The embryogenic calluses of all three Allium species were similar in appearance. For all accessions tested plants could be regenerated at a high frequency from this compact callus through somatic embryogenesis, when using kinetin supplemented MS medium (regeneration medium). Addition of abscisic acid to the regeneration medium stimulated the formation of both somatic embryos and shoots for a number of varieties. Concerning shoot regeneration from callus cultures, significant differences existed between genotypes of all accessions except one.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-5060
    Keywords: Box-Cox transformation ; maximum likelihood ; mixtures of normal distributions ; perennial ryegrass ; Lolium perenne ; ploidy breeding ; diploid (unreduced) pollen grains
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The size of pollen grains is commonly used to indicate the ploidy level of pollen grains. In this paper observations of the diameter of pollen grains are evaluated from one diploid accession of perennial ryegrass (Lolium perenne L.), which was expected to produce diploid (unreduced) pollen grains in addition to haploid pollen grains. The considerable overlap of the diameter distributions of haploid and diploid pollen grains severely hampers the accurate estimation of the proportion of diploid pollen grains. To overcome this problem we develop in this paper a statistical normal mixture model and we describe a method to test for the production of diploid pollen grains from a diploid parent, and to estimate the proportion of diploid pollen grains.
    Type of Medium: Electronic Resource
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