ISSN:
1432-0584
Keywords:
DNA
;
analysis
;
Gene rearrangement
;
PCR
;
Fixed material
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary In the present study methods are described for the analysis of the genomic structure of DNA isolated from tissues that have been stored up to several years as air-dried or stained bone marrow smears, bone marrow biopsies in “Histicon”, chromosomal preparations in fixative or as formalin fixed tissues embedded in paraffin. By application of the new techniques clonal B- and T-cell disorders, Philadelphia chromosome translocations and ras oncogene mutations in pancreatic carcinomas could be detected. Thus, these DNA extraction procedures may open new avenues to pathological archives and enable the analysis of samples when fresh material is not available.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01702923
Permalink