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  • 1
    Electronic Resource
    Electronic Resource
    Field desorption mass spectrometry of poly(olefin sulfones) (1986)
    s.l. : American Chemical Society
    Macromolecules 19 (1986), S. 1770-1772 
    Details
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ma00160a050
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  • 2
    Electronic Resource
    Electronic Resource
    A general approach to desalting oligosaccharides released from glycoproteins (1998)
    Springer
    Glycoconjugate journal 15 (1998), S. 737-747 
    Details
    ISSN: 1573-4986
    Keywords: desalting oligosaccharides ; graphitized carbon ; solid phase ; PNGase F ; electrospray mass spectroscopy ; HPAEC-PAD, high performance anion exchange chromatography with pulsed amperometric ; ESI-MS, electrospray ionization mass spectrometry ; PNGase F ; HexNAc, N-acetyl hexosamine ; Hex, hexose ; GalNAc4S,N-acetylgalactosamine-4-sulfate ; IduA, iduronic acid ; TFA ; SDS, sodium dodecyl sulfate ; NP40, Nonidet P40
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Desalting of sugar samples is essential for the success of many techniques of carbohydrate analysis such as mass spectrometry, capillary electrophoresis, anion exchange chromatography, enzyme degradation and chemical derivatization. All desalting methods which are currently used have limitations: for example, mixed-bed ion-exchange columns risk the loss of charged sugars, precipitation of salt by a non-aqueous solvent can result in co-precipitation of oligosaccharides, and gel chromatography uses highly crosslinked packings in which separation of small oligosaccharides is difficult to achieve. We demonstrate that graphitized carbon as a solid phase extraction cartridge can be used for the purification of oligosaccharides (or their derivatives) from solutions containing one or more of the following contaminants: salts (including salts of hydroxide, acetate, phosphate), monosaccharides, detergents (sodium dodecyl sulfate and Triton X-100), protein (including enzymes) and reagents for the release of oligosaccharides from glycoconjugates (such as hydrazine and sodium borohydride). There is complete recovery of the oligosaccharides from the adsorbent which can also be used to fractionate acidic and neutral glycans. Specific applications such as clean-up of N-linked oligosaccharides after removal by PNGase F and hydrazine, desalting of O-linked glycans after removal by alkali, on-line desalting of HPAEC-separated oligosaccharides and β-eliminated alditols prior to electrospray mass spectrometry, and purification of oligosaccharides from urine are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006983125913
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  • 3
    Electronic Resource
    Electronic Resource
    Analyzing glycoproteins separated by two-dimensional gel electrophoresis (1998)
    Weinheim : Wiley-Blackwell
    Electrophoresis 19 (1998), S. 981-988 
    Details
    ISSN: 0173-0835
    Keywords: Glycoprotein ; Two-dimensional polyacrylamide gel electrophoresis ; α1-Antitrypsin ; α2-HS Glycoprotein ; Protein isoforms ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Two-dimensional (2-D) electrophoresis is the preferred method for separating the glycoforms of proteins. The isoforms usually present as ‘trains’ of spots in the first dimension and may also differ in molecular weight. The primary goal for analyzing the carbohydrate content of glycoprotein spots is to understand the ‘rules’ which govern the migration of glycoproteins in 2-D electrophoresis. These rules can then be used to produce predictive vectors to interpret changes in glycosylation patterns. Techniques for the analysis of oligosaccharides released from glycoproteins which have been electroblotted to PVDF membrane after one-dimensional (1-D) and 2-D preparative gel electrophoresis are described. The oligosaccharides are removed enzymatically (PNGase F of N-linked oligosaccharides) or chemically (β-elimination of O-linked oligosaccharides) and separated by high performance anion exchange chromatography (HPAEC-PAD) and identified by electrospray ionization mass spectrometry (ESI-MS) or analyzed directly by ESI-MS. After enzymic removal of the N-linked oligosaccharides the protein spots can be further analyzed by Edman sequence tagging for identification and quantitation of the protein and by acid hydrolysis for monosaccharide analysis of the O-linked oligosaccharides. These approaches have been proved on 1-D PAGE electroblotted bovine fetuin and human glycophorin A and then used to analyze two abundant proteins which separate as glycoforms on 2-D PAGE preparative narrow range (pH 4.5-5.5) blots of human plasma: α2-HS glycoprotein (human fetuin) and α1-antitrypsin (α1-protease inhibitor). It is apparent that both the macroheterogeneity (site occupation) and microheterogeneity (diversity of structures) of the glycosylation contribute to the separation of protein isoforms in 2-D PAGE.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150190613
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Time-of-flight/time-of-flight tandem mass spectrometry of multiple incident ions (1991)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 26 (1991), S. 915-916 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210261021
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  • 5
    Electronic Resource
    Electronic Resource
    A tandem time-of-flight mass spectrometerDedicated to Professor Jim Shannon in recognition of his contributions to organic mass spectrometry. (1992)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 27 (1992), S. 1077-1083 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A tandem time-of-flight mass spectrometer Incorporating a fast atom bombardment source has been designed and constructed for the purpose of studying collision-induced decomposition spectra. A deflection of the secondary ion beam is used to form the ion packets for time-of-flight analysis. A floating collision cell that represents a potential barrier to ions transversing the time-of-fiight tube enables ionic and neutral fragments from collision-induced decomposition reactions to be separated in time from the parent ion. The instrument operating in the tandem mode is capable of performing charge exchange and collision-induced decomposition studies of both positive and negative incident ions.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210271018
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  • 6
    Electronic Resource
    Electronic Resource
    C60-assisted laser desorption-ionization mass spectrometry (1994)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 29 (1994), S. 512-515 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210290912
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    Paper (German National Licenses)
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