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  • 1
    Electronic Resource
    Electronic Resource
    Mapped DNA probes from loblolly pine can be used for restriction fragment length polymorphism mapping in other conifers (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 279-282 
    Details
    ISSN: 1432-2242
    Keywords: Pinus taeda L. ; Conifers ; Restriction fragment length polymorphisms (RFLPs) ; Genetic mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A high-density genetic map based on restriction fragment length polymorphisms (RFLPs) is being constructed for loblolly pine (Pinus taeda L.). Consequently, a large number of DNA probes from loblolly pine are potentially available for use in other species. We have used some of these DNA probes to detect RFLPs in 12 conifers and an angiosperm. Thirty complementary DNA and two genomic DNA probes from loblolly pine were hybridized to Southern blots containing DNA from five species of Pinus (P. elliottii, P. lambertiana, P. radiata, P. sylvestris, and P. taeda), one species from each of four other genera of Pinaceae (Abies concolor, Larix laricina, Picea abies, and Pseudotsuga menziesii), one species from each of three other families of Coniferales [Sequoia sempervirens (Taxodiaceae), Torreya californica (Taxaceae) and Calocedrus decurrens (Cupressaceae)], and to one angiosperm species (Populus nigra). Results showed that mapped DNA probes from lobolly pine will cross-hybridize to genomic DNA of other species of Pinus and some other genera of the Pinaceae. Only a small proportion of the probes hybridized to genomic DNA from three other families of the Coniferales and the one angiosperm examined. This study demonstrates that mapped DNA probes from loblolly pine can be used to construct RFLP maps for related species, thus enabling the opportunity for comparative genome mapping in conifers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223632
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  • 2
    Electronic Resource
    Electronic Resource
    Inheritance of restriction fragment length polymorphisms and random amplified polymorphic DNAs in coastal Douglas-fir (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 758-766 
    Details
    ISSN: 1432-2242
    Keywords: Douglas-fir ; Genetic mapping ; RFLP RAPD ; allozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A total of 225 new genetic loci [151 restriction fragment length polymorphisms (RFLP) and 74 random amplified polymorphic DNAs (RAPD)] in coastal Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco var. menziesii] have been identified using a three-generation outbred pedigree. The Mendelian inheritance of 16 RFLP loci and 29 RAPD loci was demonstrated based on single-locus segregation in a sample of F2 progeny. One RFLP locus, PtIFG2025, showed segregation distortion. Probe pPtIFG2025 is a loblolly pine cDNA probe encoding for rbcS. The 16 RFLP loci and 23 allozyme loci were also assayed in a sample of 16 Douglas-fir seed-orchard clones. Allelism was determined at 11 of the 16 RFLP loci. RFLPs were able to detect slightly more variation (4.0 alleles per locus) than allozymes (3.1 alleles per locus). The inheritance of an additional 80 RAPD loci was determined based on haploid segregation analysis of megagametophytes from parent tree 013-1. Once 200–300 markers are identified and placed on a genetic map, quantitative trait loci affecting bud phenology will be mapped.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223716
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  • 3
    Electronic Resource
    Electronic Resource
    Partial DNA sequencing of Douglas-fir cDNAs used for RFLP mapping (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 771-776 
    Details
    ISSN: 1432-2242
    Keywords: Key words Douglas-fir ; cDNA RFLP probes ; DNA sequence ; Similarity search ; Putative function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  DNA sequences from 87 Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco) cDNA RFLP probes were determined. Sequences were submitted to the GenBank dbEST database and searched for similarity against nucleotide and protein databases using the BLASTn and BLASTx programs. Twenty-one sequences (24%) were assigned putative functions; 18 of which were from plant species. Six sequences aligned with conifer genes, including genes from Douglas-fir. Similarities among the 87 sequences were revealed by analyses with FASTA, suggesting either redundancy or isoforms of the same gene. Assignment of putative functions to anonymous cDNA mapped markers will increase the understanding of structural gene organization of the Douglas-fir genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050954
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  • 4
    Electronic Resource
    Electronic Resource
    A sex-averaged genetic linkage map in coastal Douglas-fir (Pseudotsuga menziesii[Mirb.] Franco var ‘menziesii’) based on RFLP and RAPD markers (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 762-770 
    Details
    ISSN: 1432-2242
    Keywords: Key words RFLP ; RAPD ; Genetic map ; Null loci ; Gene family
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We have constructed a sex-averaged genetic linkage map in coastal Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco var ‘menziesii’) using a three-generation outcrossed pedigree and molecular markers. Our research objectives are to learn about genome organization and to identify markers associated with adaptive traits. The map reported here is comprised of 141 markers organized into 17 linkage groups and covers 1,062 centiMorgans (cM). Of the markers positioned on the map, 94 were derived from a Douglas-fir complimentary-DNA (cDNA) library that was constructed from new-growth needle tissue. Other markers include 11 Douglas-fir genomic-DNAs, 20 loblolly pine (Pinus taeda L.) cDNAs, 15 random amplified polymorphic DNAs (RAPDs) and a PCR-amplified phytochrome probe. A high degree of variation was detected in each of the two parents of our mapping population, and many of the restriction fragment length polymorphism (RFLP) and RAPD phenotypes were complex. Marker data were analyzed for linkage using mapping software JOINMAP version 2.0.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050953
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  • 5
    Electronic Resource
    Electronic Resource
    Inheritance of RFLP loci in a loblolly pine three-generation pedigree (1991)
    Springer
    Theoretical and applied genetics 83 (1991), S. 238-242 
    Details
    ISSN: 1432-2242
    Keywords: Pinus taeda L. ; Restriction fragment length polymorphisms ; genome mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A high-density restriction fragment length polymorphism (RFLP) linkage map is being constructed for loblolly pine (Pinus taeda L.). Loblolly pine cDNA and genomic DNA clones were used as probes in hybridizations to genomic DNAs prepared from grandparents, parents, and progeny of a three-generation outbred pedigree. Approximately 200 probes were evaluated for their ability to detect polymorphic loci between DNAs prepared from the two parent trees, 20–1010 and 11–1060, and cut with four different restriction enzymes: BamHI, DraI, EcoRI, and HindIII. More than half of the probes detecting single- or low-copy number sequences (56%) revealed polymorphisms between the two parents with at least one restriction enzyme. If necessary, an additional hybridization to DNAs prepared from the four grandparent trees was conducted to determine the zygosity of parent trees. Ten of these probes were hybridized to progeny DNAs from this cross and, as expected, the markers were inherited as simple codominant Mendelian alleles. Four of the ten probes detected segregation of three alleles at one locus, and four probes detected more than one independently segregating locus. RFLPs can be used immediately to assess genetic diversity in conifer populations and to “efingerprint” genotypes in tree improvement programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226257
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  • 6
    Electronic Resource
    Electronic Resource
    Use of DNA markers in forest tree improvement research (1992)
    Springer
    New forests 6 (1992), S. 391-407 
    Details
    ISSN: 1573-5095
    Keywords: DNA markers ; genetic maps ; RFLPs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract DNA markers are rapidly being developed for forest trees. The most important markers are restriction fragment length polymorphisms (RFLPs), polymerase chain reaction- (PCR) based markers such as random amplified polymorphic DNA (RAPD), and fingerprinting markers. DNA markers can supplement isozyme markers for monitoring tree improvement activities such as; estimating genetic diversity in breeding populations, germplasm identification, verifying controlled crosses, and estimating seed orchard efficiencies. Because the number of DNA markers is potentially limitless, it should be possible to map individual quantitative trait loci (QTL) by linkage analysis with high-density maps. Finally, if such associations can be found, it may also be possible to design marker-assisted breeding strategies for forest trees.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00120654
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