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  • 1
    Electronic Resource
    Electronic Resource
    Inheritance and chromosomal location of Septoria passerinii resistance introgressed from Hordeum bulbosum into Hordeum vulgare (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Septoria speckled leaf blotch (SSLB), caused by Septoria passerinii, has become one of the most serious diseases of barley in the Upper Midwest region of the USA. The recombinant line 36L5 derived from a backcross of the susceptible barley cultivar ‘Emir’ and a resistant Hordeum bulbosum parent Cb2920/4/Colch was found to be resistant to S. passerinii. Two doubled haploids derived from 36L5 were backcrossed to cv.‘Emir’ to obtain two BCF2 populations for determining the inheritance of resistance to S. passerinii. BCF2 progeny and BCF2:3 families were evaluated at the seedling stage in the greenhouse for reaction to S. passerinii. BCF2 progeny and BCF2:3 families from both crosses segregated 3 : 1 (resistant : susceptible), and 1:2:1 (resistant : segregating : susceptible), respectively, indicating that the H. bulbosum-derived SSLB resistance is conferred by a single dominant gene. The H. bulbosum introgressions were positioned on chromosome 4HL by genomic and fluorescent in situ hybridizations (GISH and FISH, respectively) and by Southern hybridization with the rye repetitive sequence pSc119.2. These findings indicate that SSLB resistance in H. bulbosum has the potential to be transferred and utilized in barley breeding programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2003.00850.x
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  • 2
    Electronic Resource
    Electronic Resource
    PLASMA CATECHOLAMINE CONCENTRATIONS DURING MORPHINE WITHDRAWAL IN CONSCIOUS GUINEA-PIGS (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical and experimental pharmacology and physiology 14 (1987), S. 0 
    Details
    ISSN: 1440-1681
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: 1. Plasma noradrenaline (NA) and adrenaline (AD) concentrations and locomotor activity were measured in conscious normal and adrenalectomized guinea-pigs before and after injection of a single dose of morphine (15 mg/kg) and following precipitation of withdrawal with naloxone 2 h later. Arterial blood samples were obtained via carotid catheters inserted 3 days before experiment. Normal guinea-pigs given saline instead of morphine were used as controls.2. Increased locomotor activity occurred following naloxone injection in both intact and adrenalectomized morphine-treated guinea-pigs.3. Plasma AD concentration was significantly elevated only during the naloxone- precipitated withdrawal period, whereas plasma NA concentration was also elevated during the morphine treatment period. No significant changes occurred in saline-treated guinea-pigs given naloxone.4. In adrenalectomized guinea-pigs AD did not reach detectable levels in the plasma at any time during the experiments, thus showing that the increased plasma AD level during morphine withdrawal in normal animals reflected adrenal medullary release.5. It was concluded that measurements of plasma AD concentration and locomotor activity in guinea-pigs should be useful for the study of the interactions between behavioural and autonomic mechanisms involved in opiate dependence and withdrawal, since the effects of morphine and morphine withdrawal on the autonomic nervous system are clearly different in this species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1440-1681.1987.tb01883.x
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  • 3
    Electronic Resource
    Electronic Resource
    Tachykinin antagonists inhibit the morphine withdrawal response in guinea-pigs (1991)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 343 (1991), S. 283-288 
    Details
    ISSN: 1432-1912
    Keywords: Morphine withdrawal ; Substance P ; Tachykinin antagonists ; Locomotor activity ; Guinea-pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary This study was an investigation of the effects of the tachykinin antagonists, spantide and (d-Pro4, d-Trp7, 9, 10)substance P 4-11, injected intracerebroventricularly (ICV), on the locomotor and behavioural responses of guinea-pigs to substance P (SP) injected ICV and to naloxone-induced morphine withdrawal. SP, 50 nmol, produced increased locomotor activity and behaviour that mimicked the response induced by injection of naloxone hydrochloride, 15 mg/kg, in guinea-pigs treated 2 h previously with morphine sulphate, 15 mg/kg. Spantide or (d-Pro4, d-Trp7, 9, 10)SP4-11, 10 nmol, reduced the locomotor and behavioural responses to SP and to morphine withdrawal. The results support the suggestion that SP or a related tachykinin might be a mediator of the opioid withdrawal response in the central nervous system as has been proposed for the enteric nervous system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00251127
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  • 4
    Electronic Resource
    Electronic Resource
    Locating introgressions of Hordeum bulbosum chromatin within the H. vulgare genome (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 27-31 
    Details
    ISSN: 1432-2242
    Keywords: Key words Hordeum vulgare ; Hordeum bulbosum ; Leaf rust resistance ; Gene introgression ; In situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Several disease-resistant recombinants between barley (Hordeum vulgare) and bulbous barley grass (H. bulbosum) have been obtained in recent years, but the process of characterization is often laborious and time-consuming. In order to improve the identification and chromosomal location of introgressed chromatin from H. bulbosum into the barley genome, we employed sequential genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH). GISH enabled us to establish that an introgression was present in the disease-resistant recombinant line, and the subsequent use of FISH, with a short oligonucleotide sequence as probe, allowed us to locate the introgression on the long arm of barley chromosome 2H. These data were confirmed using RFLP probes that hybridize to barley chromosome 2HL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00002904
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