ISSN:
1573-6784
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary The release of chloramphenicol acetyl transferase (CAT) from a recombinant Escherichia coli strain by ultrasonication and the French press was compared. French pressing disrupted all cells in suspension whereas only a fraction of the cells was disrupted following sonication. The level of CAT released was highest when cells were totally disrupted. Additional treatment with the detergent Triton X-100 was necessary to maximize CAT recovery, presumably due to association of CAT with cellular debris.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00159561
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