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Molecular Properties of Drosophila Acetylcholinesterase (1983)

Zingde, S. ; Rodrigues, V. ; Joshi, S. M. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

Journal of neurochemistry 41 (1983), S. 0

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ISSN: 1471-4159

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Abstract: Two distinct classes of acetylcholinesterase (AChE) from the fruit fly Drosophila melanogaster are reported: a soluble species that shows heterogeneity of forms and a particulate species. The subunit composition of the particulate enzyme was studied using the active site label [3H]diisopropylfIuorophosphate. Comparison of the electrophoretic patterns on nondenaturing gels using the activity stain and the active site label shows that the label is specific to AChE. The smallest active site-containing subunit of the enzyme is a monomer of —60,000 daltons MW. Two such units are linked by disulphide bonds to produce a dimer of about 110,000 daltons. Another monomeric form of MW —64,000 daltons, although present, does not participate in the dimerisation. The particulate enzyme when solubilised exists as a 9–10S species as determined by sucrose gradient centrifugation. This species has a MW 〉 200,000, as shown by its behaviour on a coarse-bead Sephadex-G200 column. Electrophoretic analysis suggests a MW of nearly 250,000 daltons for this form. Thus, this species is likely to be a tetramer. One possibility is that this tetramer is made up of two units of 64,000 daltons each and a dimer of 110,000 daltons. Preliminary data on mutant enzymes that support such a possibility are also presented.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1471-4159.1983.tb00818.x

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Studies in swelling of cotton. I. Neutral salt technique to determine the true swelling parameters in cadoxen (1976)

Achwal, W. B. ; Joshi, S. M. ; Karmakar, S. R.

New York, NY [u.a.] : Wiley-Blackwell

Journal of Applied Polymer Science 20 (1976), S. 2347-2354

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ISSN: 0021-8995

Keywords: Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics

Notes: This paper describes the swelling of cotton fiber in cadoxen containing 4.6% cadmium (w/w) and 30% ethylenediamine (w/w). A special neutral salt technique is described involving the analysis of extract as well as mother liquor which enables the determination of mechanically held liquor and hence the values of true absorption. The percentage true weight swelling values are much lower than the percentage apparent weight swelling as measured by centrifuge technique. The effect of variation in material-to-liquor ratio on the true absorption of cadmium and ethylenediamine is also investigated.

Additional Material: 1 Ill.