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  • 1
    Electronic Resource
    Electronic Resource
    In situ reflectance monitoring of InP/InGaAsP films grown by metalorganic vapor phase epitaxy (1996)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 69 (1996), S. 928-930 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We report the use of in situ reflectance spectroscopy for real-time monitoring of the metalorganic vapor phase epitaxy (MOVPE) growth of InGaAsP films. In situ optical measurements have not been previously reported for this materials system because of its strong absorption in the spectral range of commonly available detectors (λ〈1100 nm). To acquire reflectance data beyond the absorption regions of these films we used a grating spectrometer with a Si/PbS dual detector having a wavelength range of 400–2500 nm. Measurements were made during growth of InP/InGaAs/InP double heterostructures and 1.3 μm InGaAsP MQW laser device structures. The multiwavelength reflectance data enabled extraction of the film optical constants, determination of deposition rates to better than 1%, and provided nanometer scale thickness sensitivity for MQW samples. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.116946
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  • 2
    Electronic Resource
    Electronic Resource
    Differences in the molecular basis of resistance to the cyclohexanedione herbicide sethoxydim in Lolium multiflorum (2005)
    Oxford, UK : Blackwell Science Ltd
    Weed research 45 (2005), S. 0 
    Details
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Resistance to the cyclohexanedione (CHD) herbicide sethoxydim was investigated in two UK Lolium multiflorum populations, Yorks A2 and PYL. Resistance screening experiments demonstrated a qualitative difference in the responses of the two populations to sethoxydim, suggesting that the molecular basis of resistance between them was different. After treatment, Yorks A2 plants were either alive (78% of sample tested) or dead (22% tested) but plants of the PYL population showed two further intermediate categories of response. The level of acetyl-CoA carboxylase (ACCase) insensitivity directly correlated with the degree of resistance at the whole plant level, indicating that the molecular basis of resistance is associated with differences in ACCase sensitivity in each population. Direct sequencing of the carboxyl transferase domain of the ACCase gene showed that an Ile-418-Leu substitution in the L. multiflorum chloroplastic ACCase (GenBank accession number 〈accessionId ref="info:ddbj-embl-genbank/AY710293"〉AY710293) confers resistance to sethoxydim in Yorks A2. This corresponds to amino acid residue 1781 in the Alopecurus myosuroides full ACCase sequence. This is the first report of this mutation in this L. multiflorum, which has also been reported in four other grass-weeds, including L. rigidum. However, no amino acid substitutions were found to be specifically associated with the resistant phenotypes in the PYL population and the molecular basis of resistance in this population remains to be resolved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3180.2005.00478.x
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic diversity in Echinochloa spp. collected from different geographic origins and within rice fields in Côte d'Ivoire (2002)
    Oxford, UK : Blackwell Science Ltd
    Weed research 42 (2002), S. 0 
    Details
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Diversity studies of Echinochloa spp. are complicated by problems in taxonomy and species identification, caused by the existence of morphologically intergrading types. Six amplified fragment length polymorphism (AFLP) primer combinations and five microsatellites were used to assess variation in 24 samples morphologically identified as E. crus-galli, E. colona and E. crus-pavonis, from Bangladesh, India, Colombia, Costa Rica, Côte d'Ivoire and Philippines. Out of 909 AFLP bands generated, 775 were polymorphic. Genotype diversity for the microsatellites ranged from 0.28 to 0.72. Similarity matrices were calculated using Jaccard coefficient, and input into cluster and principal coordinates analyses. AFLP and microsatellite results were highly correlated. Echinochloa crus-pavonis and E. crus-galli were intermixed, consistent with the view that E. crus-galli occurs as numerous intergrading races in the four countries (Bangladesh, India, Côte d'Ivoire and Philippines). The E. colona samples clustered as a distinct group. In 15 samples of E. crus-pavonis collected from rice fields in a valley in Côte d'Ivoire (over a 2-km distance), four different genotypes were found in a 4 m × 4 m area. These results suggest that AFLPs and SSRs may be useful not only for discriminating genotypes and studying population structure but also for helping to resolve taxonomic relationships in Echinochloa spp.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3180.2002.00300.x
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  • 4
    Electronic Resource
    Electronic Resource
    Studies on the genetic basis of resistance to potato leaf roll virus, potato virus Y and potato virus X in Solanum brevidens using somatic hybrids of Solanum brevidens and Solanum tuberosum
    Amsterdam : Elsevier
    Plant Science 69 (1990), S. 95-101 
    Details
    ISSN: 0168-9452
    Keywords: Solanum brevidens ; potato ; somatic hybrids ; virus resistance
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(90)90107-Y
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  • 5
    Electronic Resource
    Electronic Resource
    An analysis of genetic diversity in coconut (Cocos nucifera) populations from across the geographic range using sequence-tagged microsatellites (SSRs) and AFLPs (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 764-771 
    Details
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Genetic diversity in 31 individuals from 14 coconut populations across the entire geographic range (2–3 individuals per population) was assessed using sequence-tagged microsatellites (or simple sequence repeats, SSRs) and amplified fragment length polymorphism (AFLP). From the 39 SSR primer sets tested, only two gave patterns that could not be scored and used in the data analysis. The remainder included five SSRs that gave double-locus profiles in which one locus could still be scored separately. The 37 SSRs revealed between 2 and 16 alleles per locus and a total of 339 alleles in the 14 populations. Gene diversity (D = 1-Σpi 2) ranged from 0.47 to 0.90. Two of the four Dwarf populations were homozygous at all 37 loci, which is consistent with their autogamous (self-fertilising) reproduction. One Dwarf population was heterozygous at one locus but the other (Niu Leka Dwarf), which is known to be cross-pollinating, showed high levels of heterozygosity. Generally, diversity was higher in populations from the South Pacific and South East Asia. Three SSR loci (CNZ46, CN2A5, CN11E6) gave distinct genotypes for all but two populations. The East African populations had higher heterozygosities than those from West Africa, and the populations from Tonga and Fiji generally had distinct alleles from those of the South Pacific. AFLP analysis with 12 primer combinations gave a total of 1106 bands, of which 303 were polymorphic (27%). Similarity matrices were constructed from the two data sets using the proportion of shared alleles for SSRs and a Jaccard coefficient for AFLPs. In each case cluster and principal co-ordinates analyses were performed, with the resultant dendrograms and plots revealing similar relationships among the populations for both approaches. There was generally a good separation of populations, and phenetic relationships were in agreement with those previously shown by RFLPs. The use of SSRs and AFLPs in genetic-diversity analysis for the establishment of germplasm collections is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051350
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  • 6
    Electronic Resource
    Electronic Resource
    Cytogenetics of Lolium perenne (1983)
    Springer
    Theoretical and applied genetics 65 (1983), S. 149-156 
    Details
    ISSN: 1432-2242
    Keywords: Lolium perenne ; Meiosis ; Chiasma distribution ; U-type exchange
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In two contrasting types of S5 inbred Lolium perenne a correlation has been found between the distribution pattern of chiasmata within bivalents and that of meiotic U-type exchange. The relationship between the two processes is discussed, in terms of inbreeding effects upon chiasma redistribution, and mention is also made of some other types of chromosome breakages which arise following enforced self-pollination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264883
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  • 7
    Electronic Resource
    Electronic Resource
    From tumour to tuber; tumour cell characteristics and chromosome numbers of crown gall-derived tetraploid potato plants (Solanum tuberosum cv. ‘Maris Bard’) (1983)
    Springer
    Theoretical and applied genetics 66 (1983), S. 169-172 
    Details
    ISSN: 1432-2242
    Keywords: Agrobacterium tumefaciens ; Crown gall ; Chromosomes ; Potato ; Variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Agrobacterium tumefaciens strains, known to induce tobacco crown galls that spontaneously develop shoots, were used to induce galls on cultured shoots of a tetraploid potato cultivar (Solanum tuberosum cv. ‘Maris Bard’). Shoots also appeared spontaneously from the induced potato galls, although only after 2–4 months. The shoots were excised and cultured separately. Some of these frequently developed side-shoots from their axillary buds. They did not form roots and they produced opines, a strong indication that they were transformed and carried T-DNA. Grafts of the transformed plants were still able to develop tubers. Most of the tumour-derived shoots, however, formed roots, did not produce opines and were indistinguishable from the parental plants on the basis of morphology and chromosome numbers (48 chromosomes per cell). The results are discussed in relation to the origin of previously described variation among protoplast-derived potato plants and with respect to genetic engineering of tetraploid potato cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265193
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  • 8
    Electronic Resource
    Electronic Resource
    Chromosome variation in wheat plants regenerated from cultured immature embryos (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 249-255 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Plant regeneration ; Chromosome variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A cytological study has been made of plants regenerated from cultured immature embryos of four wheat cultivars (Triticum aestivum, 2n = 6x = 42). In total, 29% of the 192 plants examined were aneuploid with a range in chromosome numbers of 38–45. Evidence of chromosome structural changes was also found. This variation occurred in regenerants of all four cultivars, but there were large differences in the proportions of aneuploids arising from individual cultures which meant that no significant differences could be demonstrated between cultivars. Chromosome abnormalities were present in plants regenerated both from embryogenic cultures and from cultures in which the origin of shoots could not be distinctly defined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317047
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  • 9
    Electronic Resource
    Electronic Resource
    Genetic modification of potato development using Ri T-DNA (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 440-446 
    Details
    ISSN: 1432-2242
    Keywords: Agrobacterium rhizogenes ; Potato ; Somaclonal variation ; Genetic manipulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Forty-two potato plants were regenerated from a hairy-root line obtained after infection of a shoot of Solanum tuberosum cv ‘Desiree’ with Agrobacterium rhizogenes strain LBA 9402 (pRil855). Transformed plants were uniform and had a distinct phenotype and development compared with untransformed controls. Their growth was vigorous, especially early in their development, their roots were abundant and showed reduced geotropism, their leaves were slightly crinkled and glossy and they produced longer tubers with more frequent, prominent eyes. Cytological examination showed that ten of the forty-two transformed plants had either 47 or 49 chromosomes instead of the normal 48. In two of these aneuploids structural changes were observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273752
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  • 10
    Electronic Resource
    Electronic Resource
    Genetic manipulation in cultivars of oilseed rape (Brassica napus) using Agrobacterium (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 325-329 
    Details
    ISSN: 1432-2242
    Keywords: A. tumefaciens ; A. rhizogenes ; Brassica napus ; Plant development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The response of oilseed rape cultivars to infection with Agrobacterium tumefaciens and A. rhizogenes and the possibility of regenerating genetically transformed oilseed rape plants were examined. The frequency at which Agrobacterium induced galls or hairy-roots on in vitro cultured plants ranged from 10% to 70%, depending on the cultivar. From galls induced by the tumorigenic strain T37, known to be strongly shoot inducing on tobacco, roots developed frequently. Occasionally, shoots formed and some of these produced tumour cell specific nopaline. Attempts to grow the transformed shoots into plants have so far been unsuccessful. Whole plants transformed with Ri-T-DNA, however, were regenerated. These had crinkled leaves and abundant, frequently branching roots that showed reduced geotropism, similar to previously isolated Ri T-DNA transformed tobacco and potato plants. The transformed oilseed rape plants flowered, but failed to form seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252075
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