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  • 1
    Digitale Medien
    Digitale Medien
    Mechanism of calcium-dependent salt tolerance in cells of Nitellopsis obtusa: role of intracellular adenine nucleotides (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 13 (1990), S. 0 
    Details
    ISSN: 1365-3040
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Abstract. The tonoplasts of internodal cells of Nitellopsis were removed by perfusing the vacuoles with media containing a Ca2 chelator, EGTA. Treatment of tonoplast-free cells with 100 mol m3 NaCl induces a large membrane depolarization, a drastic decrease in the membrane resistance and an increase in Na+ influx. These events are identical to those that occur in intact cells subjected to high NaCl. These responses to NaCl are prevented if 10 mol m3 Ca2+ is supplied together with 100 mol m3 NaCl. The protective effect of Ca2+ is evident only when the intracellular ATP concentration exceeds 0.1 mol m3 and does not occur full when the intracellular ATP is removed. AMP at concentrations greater than 0.5 mol m3 or 0.25 mol m3 AMPPNP can replace ATP. It is concluded that ATP does not act as an energy source nor as a substrate for protein phosphorylation. ATP seems to exert its effects as a coeffector with Ca2+ in regulating the Na+ permeability of the plasma membrane.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-3040.1990.tb01290.x
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  • 2
    Digitale Medien
    Digitale Medien
    Changes in sodium and potassium in Nitellopsis cells treated with transient salt stress (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 11 (1988), S. 0 
    Details
    ISSN: 1365-3040
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Abstract. Nitellopsis cells grown in fresh water have a relatively low cytoplasmic Na+ (11 mol m−3) and high cytoplasmic K+ (90 mol m−3) content. A 30-min treatment with 100 mol m−3 external NaCl resulted in a high [Na+]c (90 mol m−3) and a low [K+]c (33 mol m−3), Subsequent addition of external Ca2+ (10 mol m−3) prevented Na+ influx and then [Na+]c decreased slowly. Changes in [K+]c were opposite to [Na+]c. During the recovery time vacuolar Na+ increased, while vacuolar K+ decreased. Since all these processes proceeded also under ice-cold conditions, the restoration of original cytoplasmic ion compositions is suggested to be a passive nature. The notion that the passive movement of ions across the tonoplast can act as an effective and economic mechanism of salt tolerance under transient or under mild salt stress conditions is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/1365-3040.ep11604878
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  • 3
    Digitale Medien
    Digitale Medien
    Polyamines, indole-3-acetic acid and abscisic acid in rice phloem sap (1994)
    Springer
    Plant growth regulation 15 (1994), S. 125-128 
    Details
    ISSN: 1573-5087
    Schlagwort(e): rice ; Oryza sativa ; phloem sap ; polyamine ; abscisic acid ; auxin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Putrescine, spermidine, spermine and cadaverine have been identified and quantified in rice phloem sap and shoot extracts by HPLC. It is suggested that diamines, putrescine and cadaverine, easily migrate into the phloem, while movement of a triamine, spermidine, and a tetramine, spermine, tend to be restricted. Spermine especially seems to be the most immobile among polyamines. Thus it is indicated that movement of polyamines into phloem is decreased with increasing number of amino groups. Indole-3-acetic acid and abscisic acid in rice phloem sap were also analyzed by HPLC and it is suggested that indole-3-acetic acid is transported freely into phloem, while abscisic acid is much more actively exuded into phloem.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00024101
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  • 4
    Digitale Medien
    Digitale Medien
    Salt tolerance inNitellopsis obtusa (1986)
    Springer
    Protoplasma 135 (1986), S. 155-161 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Calcium ion ; Membrane potential ; Membrane conductance ; Na+/K+ exchange ; Nitellopsis obtusa ; Salt tolerance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The mechanism of salt tolerance was studied using isolated internodal cells of the charophyteNitellopsis obtusa grown in fresh water. When 100 mM NaCl was added to artificial pond water (0.1 mM each of NaCl, KC1, CaCl2), no cell survived for more than one day. Within the first 30 minutes, membrane potential (Em) depolarized and membrane resistance (Rm) decreased markedly. Simultaneously, cytoplasmic Na+ increased and K+ decreased greatly. At steady state the increase in Na+ content was roughly equal to the decrease in K+ content. The Cl− content of the cytoplasm did not change. These results suggest that Na+ enters the cytoplasm by exchange with cytoplasmic K+. Both the entry of Na+ and the exit of K+ are assumed to be passive and the latter being caused by membrane depolarization. Vacuolar K+, Na+, and Cl− remained virtually constant, suggesting that rapid influx of Na+ from the cytoplasm did not occur. In 100 mM NaCl containing 10 mM CaCl2, membrane depolarization, membrane resistance decrease and changes in cytoplasmic [Na+] and [K+] did not occur, and cells survived for many days. When cells treated with 100 mM NaCl were transferred within 1 hour to 100 mM NaCl containing 10 mM CaCl2, Em decreased, Rm increased, cytoplasmic Na+ and K+ returned to their initial levels, and cells survived. Two possible mechanisms for the role of Ca2+ in salt tolerance inNitellopsis are discussed; one a reduction in plasmalemma permeability to Na+ and the other a stimulation of active Na+-extrusion.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01277008
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  • 5
    Digitale Medien
    Digitale Medien
    ATP is essential for calcium-induced salt tolerance inNitellopsis obtusa (1987)
    Springer
    Protoplasma 138 (1987), S. 190-192 
    Details
    ISSN: 1615-6102
    Schlagwort(e): ATP ; Effector ; Calcium ion ; Nitellopsis obtusa ; Salt tolerance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary InNitellopsis, salt stress causes a depolarization of the membrane potential and a decrease in the membrane resistance, both of which can be prevented by the addition of calcium to the external medium (Katsuhara andTazawa 1986). The effect of calcium is also observed in tonoplast-free cells containing ATP. However, in the absence of intracellular ATP, addition of external calcium is ineffective. AMP, AMPPNP, and GTP can replace ATP in supporting the calcium effect.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01281029
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  • 6
    Digitale Medien
    Digitale Medien
    Detection and Molecular Analysis of Plant- and Insect-Associated Bacteria Harboring Aconitate Isomerase Involved in Biosynthesis of Trans-Aconitic Acid as Antifeedant in Brown Planthoppers (1997)
    Springer
    Current microbiology 35 (1997), S. 97 -102 
    Details
    ISSN: 1432-0991
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract. The activity of aconitate isomerase, which is involved in the biosynthesis of trans-aconitic acid as antifeedant in brown planthoppers, was detected in Pseudomonas fluorescens LRB3W1 and Pseudomonas putida MAFF301685 but not in Pseudomonas putida MAFF301684. The enzyme activity was induced in the presence of trans-aconitate, and therefore bacteria showing the enzyme activity were easily detected by their ability to grow on the minimal medium containing trans-aconitate as the sole carbon source (ACO agar medium). Experiments on growth of plant- or insect-associated bacteria on ACO agar medium showed that most of the Gram-negative bacteria displayed the aconitate isomerase activity unlike most of the Gram-positive bacteria isolated mainly from insects. Mini-Tn5 transposon derivatives of P. fluorescens LRB3W1 lacking completely or partially their ability to grow on ACO agar medium were obtained. Southern blot analysis with a mini-Tn5 DNA probe definitely showed that the genes responsible for the biosynthesis of aconitate isomerase present on chromosomal DNA. Thus, it was suggested that genes for aconitate isomerase biosynthesis are commonly present in Gram-negative plant- or insect-associated bacteria, and also the DNA fragments including the genes were detected in P. fluorescens LRB3W1.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002849900219
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