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  • 1
    Electronic Resource
    Electronic Resource
    In Vitro Excystation of Spironucleus muris (1996)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 43 (1996), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . In vitro excystation of Spironucleus muris cysts, purified by sequential sucrose and Percoll gradients from mouse feces, was studied. Three in vitro excystation procedures, used for Giardia, were assessed to determine the most useful method. Excystation was monitored by light microscopy and subsequently characterized by transmission and scanning electron microscopy. Spironucleus muris excysted routinely at a level greater than 90% when induced in Hanks’ balanced salt solution containing sodium bicarbonate at pH 2.0 and transferred to Tyrodes’ salt solution as an excystation medium. Similarly, high rates of excystation were recorded after induction of S. muris cysts in 0.1 M potassium phosphate buffer (pH 7.0) with sodium bicarbonate and excystation in trypticase-yeast extract-iron medium (TYI medium) or phosphate-buffered saline. A lower rate and percentage of excystation were observed after induction of S. muris cysts in an aqueous hydrochloric acid solution (pH 2.0) followed by excystation in TYI medium. All excystation methods produced extremely active S. muris trophozoites with normal morphology. Nonexcysting S. muris cysts have a wall composed of an outer fibrous and an inner membranous portion. Following induction, numerous vesicles appeared in the peritrophic space. Excystation began by the cyst wall opening at one pole, and the anterior part of the trophozoite protruding from the cyst wall. The trophozoite emerged progressively from the cyst wall and the empty cyst wall appeared to collapse. Excysted trophozoites exhibited normal morphological features of S. muris trophozoites isolated from the mouse intestine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1996.tb02473.x
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  • 2
    Electronic Resource
    Electronic Resource
    Two new species of Caryospora Léger, 1904 (Apicomplexa, Eimeriidae) from accipitrid raptors (2000)
    Springer
    Systematic parasitology 46 (2000), S. 23-27 
    Details
    ISSN: 1573-5192
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Two new species of Coccidia (Apicomplexa: Eimeriidae) are described from European accipitrid raptors (Falconiformes: Accipitridae). Oöcysts of Caryospora aquilae n. sp. found in faeces of the gold eagle Aquila chrysaetos are subspherical to broad ellipsoidal and measure 43 (40–49) × 37.5 (34–39) μm. Polar granule, oöcyst residuum and micropyle are absent. Each oöcyst contains one spherical to subspherical slightly polygonal sporocyst measuring 23.8 (23–25) × 23.3 (22–25) μm. Stieda and substieda bodies are absent. The sporocyst residuum is composed of numerous small granules less than 0.5 μm in diameter dispersed randomly among the sporozoites. Sporulated oöcysts of Caryospora circi n. sp. from faeces of the marsh harrier Circus aeruginosus are widely oval, measuring 24.5 (23–25) × 21.8 (21–24) μm. A polar granule, oöcyst residuum and micropyle are absent. Each oöcyst contains one spherical to subspherical sporocyst measuring 16.2 (15–17) × 15.6 (15–17) μm. A compact granular, spherical to subspherical sporocyst residuum, 10.4 (10–11) × 8.5 (7–9), was present in 76% of measured sporocysts. In 24% of sporocysts the granules of sporocyst residuum were scattered among the sporozoites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006227911679
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  • 3
    Electronic Resource
    Electronic Resource
    The severe combined immunodeficient mouse as a definitive host for Sarcocystis muris (1999)
    Springer
    Parasitology research 85 (1999), S. 737-742 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Peroral and intraperitoneal inoculation of severe combined immunodeficient (SCID) mice with cystozoites of three coccidia of the genus Sarcocystis (Protozoa, Apicomplexa; S. dispersa, Sarcocystis sp., and S. muris) revealed that after peroral administration, only S. muris could develop in the immunodeficient mouse host. The cystozoites of S. muris transformed into gamonts and, after fertilization, performed sporulation with the production of infectious sporocysts in the small intestine of the SCID mice. Impaired immunity is probably responsible for the unusual behavior of S. muris (which is normally the heteroxenous mouse-cat parasite) in the SCID mice. We hypothesize that the phylogenetic distance between the intermediate and final hosts is the reason why cystozoites of the two other Sarcocystis species tested (S. dispersa with a mouse-owl cycle and Sarcocystis sp. with a murine rodent-snake cycle) could not develop when inoculated into SCID mice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004360050624
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  • 4
    Electronic Resource
    Electronic Resource
    Sarcocystis atheridis sp. nov., a new sarcosporidian coccidium from Nitsche's bush viper, Atheris nitschei Tornier, 1902, from Uganda (1999)
    Springer
    Parasitology research 85 (1999), S. 758-764 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Transmission experiments were performed to elucidate the life cycle of a Sarcocystis sp. found in a Nitsche's bush viper, Atheris nitscheinitschei (Serpentes: Viperidae), from Uganda. Sporocysts measuring 10.4 (10.0–11.0) × 8.0 (7.0–8.5) μm were given to laboratory mice (Crl: CD1), laboratory rats (Wistar H), and Barbary striped mice, Lemniscomys barbarus. Sarcocysts developed in the skeletal muscles of laboratory mice and L. barbarus. No sarcocyst was observed in laboratory rats. Merogony was observed in the liver of L. barbarus at 7 and 12 days postinfection. Mature sarcocysts in mice reached a length of 30 mm and did not exceed 0.9 mm in diameter at 121 DPI. The primary sarcocyst wall was 0.6–0.8 μm thick and displayed small osmiophilic knob-like protrusions that were up to 150 nm long and 90 nm wide. Two types of asexual multiplication, endodyogony and endopolygony, were found within sarcocysts. Our results indicate that the newly found Sarcocystis represents a new species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004360050627
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  • 5
    Electronic Resource
    Electronic Resource
    Immunity against Isospora suis in nursing piglets (2000)
    Springer
    Parasitology research 86 (2000), S. 861-863 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00008515
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  • 6
    Electronic Resource
    Electronic Resource
    Isospora lutrae n. sp. (Apicomplexa: Eimeriidae), a new coccidium from the European otter Lutra lutra (L.) (Carnivora: Mustelidae) from Spain (2000)
    Springer
    Systematic parasitology 47 (2000), S. 59-63 
    Details
    ISSN: 1573-5192
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Parasitological examination of European otter originating from Extremadura, Spain revealed the presence of a new isosporan species. Oöcysts of Isospora lutrae n. sp. are spherical to subspherical, 31.2 (27.5–32) × 29.6 (28–31) μm and have a smooth wall c. 1 μm thick. Sporocysts are ellipsoidal, 18.2 (17–19) × 14.4 (14–16) μm and lack Stieda and substieda bodies. A spherical sporocyst residuum is present, consisting of granules scattered among the sporozoites. Sporozoites are spindle-shaped, 12.4 × 2.5 μm and have anterior and posterior refractile bodies. Based on its unique morphologic structure and host, I. lutrae is considered to be new.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006453532286
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  • 7
    Electronic Resource
    Electronic Resource
    Four new species of Isospora Schneider, 1881 (Apicomplexa: Eimeriidae) from reptiles from the islands of Seychelles (1997)
    Springer
    Systematic parasitology 37 (1997), S. 73-78 
    Details
    ISSN: 1573-5192
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Four new Isospora species (Apicomplexa: Eimeriidae) are described from reptiles collected in Seychelles. Oöcysts of I. gardneri n. sp. from Phelsuma astriata astriata, P. sundbergi sundbergi and P. sundbergi longinsulae are ellipsoid, 28.9 (22–31) × 23.5 (18–24) μm with a rough 1.5–2 μm thick wall. A micropyle, oöcyst residuum and polar granule are absent. Sporocysts are ellipsoid, 14.9 (12.5–17) × 8.8 (8–9.5) μm, with a dome-like Stieda body, globular substieda body and a sporocyst residuum consisting of small granules; and the sporozoites have transversal striations. Oöcysts of I. seychellensis n. sp. from 3/7 Mabuya seychellensis are ellipsoid, 19.8 (17.5–21.5) × 15.3 (14.5–16) μm. A micropyle, oöcyst residuum and polar granule are absent. Sporocysts are ovoid to broadly ovoid, 11.2 (10–12) × 7.4 (7–8) μm, with Stieda and substieda bodies. A sporocyst residuum is present, consisting of small granules; and the sporozoites have distinct transverse striations. Oöcysts of I. tigris n. sp. from 1/1 Calumma tigris are ellipsoid, 22.5 (19–24) × 18 (16–20) μm. A micropyle, oöcyst residuum and polar granule are absent. Sporocysts are ovoid or ellipsoid, 13.6 (12–15) × 7 (6–8) μm, with large Stieda body and substieda bodies. A sporocyst residuum is present, consisting of numerous small granules; and the sporozoites are vermiform with distinct transverse striations. Oöcysts of I. ladiguensis n. sp. from Phelsuma sundbergi ladiguensis and P. sundbergi longinsulae are spherical to subspherical, 13.2 (12–13.5) × 12 (9–13) μm, without micropyle and oöcyst residuum but with one globular polar granule. Sporocysts ovoid, 9 (8–10) × 5.6 (5–6) μm, with dome-like Stieda and subglobular substieda body; and the sporozoites are vermiform with distinct transverse striations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005728122660
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  • 8
    Electronic Resource
    Electronic Resource
    Isospora ptyodactyli n. sp. (Apicomplexa: Eimeriidae), a new coccidian parasite of the fan-footed gecko Ptyodactylus puiseuxi Boutan, 1893 (Reptilia: Gekkonidae) from Jordan (1998)
    Springer
    Systematic parasitology 39 (1998), S. 45-48 
    Details
    ISSN: 1573-5192
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Faecal samples from 17 fan-footed geckoes Ptyodactylus puiseuxi Boutan were examined for coccidian parasites. Five geckoes (29%) were found to be passing oöcysts of the genus Isospora Schneider. Comparison with other members of the genus Isospora indicates that the coccidian found represents a new species. Sporulated oöcysts of I. ptyodactyli n. sp. are spherical or subspherical, 22.1 (19.0–24.0) × 21.2 (18.0–23.0) µm, with a shape-index (length/width) of 1.04; and a smooth and bilayered oöcyst wall, 1.0–1.5 µm thick. A micropyle, oöcyst residuum and polar granule are absent. The sporocysts are ellipsoidal, 12.2 (11.0–14.0) × 8.0 (7.5–9.0) µm, with a shape index of 1.5 (1.4–1.9). Stieda and substieda bodies are present, the Stieda body being knob-like and the substieda body spherical to subspherical. A sporocyst residuum is present and composed of numerous granules of different size scattered among the sporozoites. The sporozoites are vermiform, with a slightly granulated surface appearance, and are arranged head to tail within the sporocyst. Most oöcysts have still to sporulate when excreted; sporulation was completed within 12 h at 25 °C. Endogenous development occurs inside the nuclei of enterocytes in the small intestine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005888121199
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