ISSN:
0263-6484
Keywords:
HMG1
;
HMG2
;
histone H10
;
normal human leukocytes
;
leukemic cell lines
;
differentiation
;
proliferation
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
Changes in the levels of chromosomal high-mobility group proteins HMG1, HMG2 and histone H10 were investigated in blood cells of various types, proliferation activity and stage of differentiation. The relative amounts of proteins HMG1, HMG2 and histone H10 were evaluated densitometrically by SDS-PAGE of 5 per cent w/v perchloric acid extracts of blood cells. Concerning the HMG1 and HMG2, the main conclusions were: the expression of these HMG proteins was higher in malignant cells, namely leukemia cell lines, then in lymphocytes or granulocytes and the distribution of HMG1 and HMG2 was highly cell-specific. In comparison with lymphoid cells, the levels of HMG1/2 were higher in myeloid cells. The results revealed that in myeloid cells HMG2 prevails over HMG1. There was no direct correlation between HMG1/2 expression and proliferation activity. The levels of HMG1/2 did not depend on the transcription of chromatin either. However, there was some connection between irreversibly differentiated nonproliferating cells and a loss of HMG1/2 proteins. Reversibly differentiated leukemic cells retain their HMG1/2 levels. Similarly to HMG1/2, H10 showed a strong cell specificity. The level of H10 was different in the various blood cell types. As compared with lymphoid cells, the level of H10 was several-fold higher in myeloid cells, regardless of whether they were normal or malignant. Moreover, there was an accumulation of H10 in differentiating HL-60 cells accompanied by only a slight decline in cell proliferation; this agrees with the idea that H10 expression is not directly associated with the inhibition of cell growth. Rather higher expression of H10 is related to changes during cell differentiation.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/cbf.290130209
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